Summary
The hydrolysis and fermentation of gelatin in the presence of a carbohydrate by gelatin-adapted mixed anaerobic bacterial populations in putatively carbon-limited chemostat cultures is investigated. It was shown that the degradation of the protein is progressively retarded with increasing dilution rates, as well as with increased concentrations of carbohydrates present in the feed as a second substrate. That this is not due to high concentrations of fermentation products in the reactor was established. Moreover, the carbohydrate is totally fermented at all dilution rates. It is concluded that for optimal performance of an anaerobic digestion system purifying waste waters containing carbohydrate/protein mixtures, fermentation of carbohydrates should be spatially separated from hydrolysis and fermentation of the protein.
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References
Andel JG van, Breure AM (1984) Anaerobic waste water treatment. Trends Biotechnol 2:16–20
Andel JG van, Zoutberg GR, Crabbendam PM, Breure AM (1985) Glucose fermentation by Clostridium butyricum grown under a self generated gas atmosphere in chemostat culture. Appl Microbiol Biotechnol 23:21–26
Atkinson DE (1968) The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry 7:4030–4034
Bodasing SJ, Robb FT, Woods DR (1983) Regulation of nitrogen catabolic enzymes in Vibrio alginolyticus. FEMS Microbiol Lett 19:175–177
Breure AM, Andel JG van (1984) Hydrolysis and acidogenic fermentation of a protein, gelatin, in an anaerobic continuous culture. Appl Microbiol Biotechnol 20:40–45
Breure AM, Andel JG van, Burger-Wiersma T, Guijt J, Verkuijlen J (1985) Hydrolysis and acidogenic fermentation of gelatin under anaerobic conditions in a laboratory scale upflow reactor. Appl Microbiol Biotechnol 21:50–54
Breure AM, Beeftink HH, Verkuijlen J, Andel JG van (1986) Acidogenic fermentation of protein/carbohydrate mixtures by bacterial populations adapted to one of the substrates in anaerobic chemostat cultures. Appl Microbiol Biotechnol 23:245–249
Cohen A, Zoetemeyer RJ, Deursen A van, Andel JG van (1979) Anaerobic digestion of glucose with separated acid production and methane formation. Water Res 13:571–580
Cohen A, Breure AM, Andel JG van, Deursen A van (1980) Influence of phase separation on the anaerobic digestion of glucose I: Maximum COD-turnover rate during continuous operation. Water Res 14:1439–1448
Cohen A, Breure AM, Andel JG van, Deursen A van (1982) Influence of phase separation on the anaerobic digestion of glucose II: Stability and kinetic responses to shock loadings. Water Res 16:449–455
Cohen A, Gemert JM van, Zoetemeyer RJ, Breure AM (1984) Main characteristics and stoichiometric aspects of acidogenesis of soluble carbohydrate containing wastewaters. Process Biochem 19:228–232
Cohen A, Distel B, Deursen A van, Breure AM, Andel JG van (1985) Role of anaerobic spore-forming bacteria in the acidogenesis of glucose: changes induced by discontinuous or low-rate feed supply. Antonie van Leeuwenhoek 51:179–192
Decker K, Rossle M, Kreusch J (1976) The role of nucleotides in the regulation of the energy metabolism of Clostridium kluyveri. In: Schlegel HG, Gottschalk G, Pfennig N (eds) Microbial production and utilization of gases. E Goltze KG, Göttingen, FRG, pp 76–83
Evans CGT, Herbert D, Tempest DW (1970) The continuous cultivation of micro-organisms. 2. Construction of a chemostat. In: Norris IR, Ribbons DW (eds) Methods in Microbiology, Vol 2. AP, London, pp 277–327
Glenn AR (1976) Production of extracellular proteins by bacteria. Ann Rev Microbiol 30:41–62
Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in Microbiology, Vol 5B. AP, London, pp 209–344
Karl DM (1980) Cellular nucleotide measurements and application in microbial ecology. Microbiol Rev 44:739–796
Long S, Mothibell MA, Robb FT, Woods DR (1981) Regulation of extracellular alkaline protease activity by histidine in a collagenolytic Vibrio alginolyticus strain. J Gen Microbiol 127:193–199
McInerney MJ, Bryant MP, Hespell RB, Costerton JW (1981) Syntrophomonas wolfei gen. nov. sp. nov. an anaerobic syntrophic, fatty acid-oxidizing bacterium. Appl Environ Microbiol 41:826–828
Pansare AC, Venugopal V, Lewis NF (1985) A note on nutritional influences on extracellular protease synthesis in Aeromonas hydrophila. J Appl Bact 58:101–104
Tempest DW (1970) The continuous cultivation of micro-organisms. 1. Theory of the chemostat. In: Norris JR, Ribbons DW (eds) Methods in Microbiology, Vol 2. AP, London, pp 259–276
Tempest DW, Hunter JR, Sykes J (1965) Magnesium limited growth of Aerobacter aerogenes in a chemostat. J Gen Microbiol 39:355–366
Tempest DW, Meers JL, Brown CM (1970) Influence of environment on the content and composition of microbial free amino acid pools. J Gen Microbiol 64:171–185
Whooley MA, O'Callaghan JA, McLoughlin AJ (1983) Effect of substrate on the regulation of exoprotease production by Pseudomonas aeroginosa ATCC 10145. J Gen Microbiol 129:981–988
Wiersma M, Hansen TA, Harder W (1978) A continuous culture study of the regulation of extracellular protease production in Vibrio SA1. Antonie van Leeuwenhoek 44:129–140
Wiersma M, Harder W (1978) Effect of environmental conditions on the production of extracellular proteolytic enzymes in Vibrio SA1. Antonie van Leeuwenhoek 44:141–155
Zoetemeyer RJ, Arnoldy P, Cohen A, Boelhouwer C (1982a) Influence of temperature on the anaerobic acidification of glucose in a mixed culture forming part of a two stage digestion process. Water Res 16:313–321
Zoetemeyer RJ, Heuvel JC van den, Cohen A (1982b) pH influence on acidogenic dissimilation of glucose in an anaerobic digester. Water Res 16:303–311
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Breure, A.M., Mooijman, K.A. & van Andel, J.G. Protein degradation in anaerobic digestion: influence of volatile fatty acids and carbohydrates on hydrolysis and acidogenic fermentation of gelatin. Appl Microbiol Biotechnol 24, 426–431 (1986). https://doi.org/10.1007/BF00294602
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DOI: https://doi.org/10.1007/BF00294602