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Mistranslation of human phosphoglycerate kinase in yeast in the presence of paromomycin

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Abstract

Missense errors in the translation of mRNAs in Saccharomyces cerevisiae were screened by looking for charge heterogeneity of proteins on two-dimensional gels resulting from the substitution of charged and neutral amino acids. No such mistranslation was detected in wild-type yeast strains grown in the presence of the translational error-inducing antibiotic paromomycin. However, paromomycin-induced mistranslation of a heterologous mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen. We suggest that the combination of error-prone translation of a heterologous mRNA, and growth in the presence of paromomycin, leads to an accumulation of mistranslated proteins that can be detected by two-dimensional gel electrophoresis.

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Grant, C.M., Tuite, M.F. Mistranslation of human phosphoglycerate kinase in yeast in the presence of paromomycin. Curr Genet 26, 95–99 (1994). https://doi.org/10.1007/BF00313794

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  • DOI: https://doi.org/10.1007/BF00313794

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