Abstract
Class I-like molecules have been immunoprecipitated from Xenopus leukocytes and erythrocytes with alloantisera directed against major histocompatibility complex (MHC)-linked antigens. The heavy chains, depending on the allele examined, have molecular weights of 40 000–44 000 of which 3000 daltons are asparagine-linked carbohydrates, probably present as one N-linked glycan. The presumed analogue of β 2-microglobulin has a molecular weight of 13 000 and bears no asparagine-linked glycans. Family studies show that the heavy chains are encoded by genes residing in or closely linked to the MHC.
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Abbreviations
- MHC:
-
major histocompatibility complex
- CML:
-
cell-mediated lympholysis
- MLR:
-
mixed leukocyte reaction
- APBS:
-
amphibian phosphate-buffered saline
- kd:
-
kilodalton
- LG:
-
Xenopus laevis — Xenopus gilli species hybrids
- IEF:
-
isoelectric focusing
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Flajnik, M.F., Kaufman, J.F., Riegert, P. et al. Identification of class I major histocompatibility complex encoded molecules in the amphibian Xenopus . Immunogenetics 20, 433–442 (1984). https://doi.org/10.1007/BF00345617
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DOI: https://doi.org/10.1007/BF00345617