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Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae

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Summary

Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNNG, to 4-NQO and triaziquone, and to mutagens 4-NQO, MNNG, and triaziquone, respectively. Integration of the gene disruption-constructs into the haploid yeast genome yielded viable null-mutants with a mutagen-sensitive phenotype. Thus, copy number of these non-essential yeast genes determines the relative resistance to certain chemical mutagens, with zero copies yielding a phenotype of mutagen sensitivity and multiple copies one of mutagen hyper-resistance, respectively.

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Authors and Affiliations

  1. Institut für Mikrobiologie der J.W. Goethe-Universität, Theodor-Stern-Kai 7, Haus 75, W-6000, Frankfurt/Main, Federal Republic of Germany

    Eckard Haase, Jörg Servos & Martin Brendel

Authors
  1. Eckard Haase
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  2. Jörg Servos
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  3. Martin Brendel
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Additional information

Communicated by K. Wolf

Dedicated to Professor Dr. R. W. Kaplan on the occasion of his 80th birthday

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Haase, E., Servos, J. & Brendel, M. Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae . Curr Genet 21, 319–324 (1992). https://doi.org/10.1007/BF00351689

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  • DOI: https://doi.org/10.1007/BF00351689

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