Summary
In this paper, we present the nucleotide sequence of a 5248 bp-long region of the mitochondrial (mt) genome of the dermatophyte Trichophyton rubrum. This region which represents about 1/4 of the total mt genome of this species reveals a compact organization of genes including: the glutaminyl tRNA, the methionyl tRNA, the cytochrome oxidase subunit 1 gene, the arginyl tRNA, the mitochondrial version of the ATPase subunit 9 gene, the cytochrome oxidase subunit II gene and a part of the NADH dehydrogenase ND4L and ND5 gene “complex”. The main features of the part of mt DNA sequenced is the non-interrupted COXI gene and the presence in the mitochondrial version of the ATPase 9 gene of a small group IA intron. The extensive amino-acid sequence similarity with the equivalent gene in Aspergillus nidulans and neuropora crassa indicates that this gene codes for a dicyclohexylcarbodiimide binding protein. The conserved arrangement of this portion of the mt genome and the presence of tRNAs between the protein-coding genes are compatible with a large polycistronic transcript processed by the excision of tRNAs, or similar secondary structures, as proposed for other fungal or mammalian mt DNAS.
Similar content being viewed by others
References
Anderson S, Bankier AT, Barrell BG, de Brujin MH, Coulson AR, Drouin J, Eperon IC, Nierlich DPD, Roe BA, Sanger F, Schreier PH, Smith AJH, Young IG (1981) Nature 290:457–465
Badillet G (1991) Les dermatophyties et les dermatophytes. Atlas clinique et biologique. Editions VARIA, Paris
Bièvre C de Dauget C, Nguyen VH, Ibrahim-Granet O (1987) Ann Inst Pasteur/Microbiol 138:719–727
Brown TA, Ray J, Waring RB, Scazzochio C and Davies RW (1984) Curr Genet 8:489–492
Burger G, Scriven NC, Machleidt W, Werner S (1982) EMBO J 1:1385–1391
Cumming DJ, Michel F, Domenico JM, McNally KL (1990) J Mol Biol 212:269–286
Dujon B, Colleaux L, Jacquier A, Michel F, Monteilhet C (1986) Extrachromosomal elements in lower eucaryotes. Plenum Publishing Corporation, new York
Flor PJ, Flaegan JB, Cech T (1989) EMBO J 11:3391–3399
Heckman JE, Hecker LI, Schwartzbach SD, Barnet WE, Baumstark B, RajBhandary UL (1978) Cell 13:83–95
Heinrich G, Lazarus CM, Bazak N, Kuntzel H (1981) Cell 23:625–633
Henikoff S (1984) Gene 28:351–359
Kochel HG, Colin M, Nazli Basak L, Kuntzel H (1981) Cell 23:625–633
Macino G, Morelli G (1983) J Biol Chem 58:13230–13235
Nelson MA, Macino G (1987) Mol Gen Genet 206:307–317
Michel F, Westhof E (1990) J Mol Biol 216:585–610
Netzker R, Kochel HG, Basak N, Kuntzel H (1982) Nucleic Acids Res 10:4783–4801
Prinzl S, Grueter F, Spelzhaus A, and Gauss D (1980) Nucleic Acids Res 8:r1-r22
Rippon JW (1988) The pathogenic fungi and the pathogenic actinomyces. Editions Saunders. Philadelphia. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Van der Boogart P, Samallo J, Agsteribbe E (1982) Nature 298:187–189
Ward M, Turner G (1986) Mol Gen Genet 205:331–338
Author information
Authors and Affiliations
Additional information
Communicated by K. Wolf
Rights and permissions
About this article
Cite this article
de Bièvre, C., Dujon, B. Mitochondrial DNA sequence analysis of the cytochrome oxidase subunit I and II genes, the ATPase9 gene, the NADH dehydrogenase ND4L and ND5 gene complex, and the glutaminyl, methionyl and arginyl tRNA genes from Trichophyton rubrum . Curr Genet 22, 229–234 (1992). https://doi.org/10.1007/BF00351730
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00351730