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Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3″) (9)

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Summary

The nucleotide sequence of the spc determinant of the Staphylococcus aureus transposon Tn554 has been determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of molecular weight 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G→A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gramnegative streptomycin/spectinomycin adenyltransferase, AAD(3″) (9), specified by the aadA determinant (Holingshead and Vapnek 1985).

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Authors and Affiliations

  1. Department of Plasmid Biology, The Public Health Research Institute of the City of New York, Inc., 455 First Avenue, 10016, New York, NY, USA

    Ellen Murphy

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  1. Ellen Murphy
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Communicated by P. Starlinger

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Murphy, E. Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3″) (9). Molec. Gen. Genet. 200, 33–39 (1985). https://doi.org/10.1007/BF00383309

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  • DOI: https://doi.org/10.1007/BF00383309

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