Skip to main content
SpringerLink
Log in

Continuous ethanol production by a flocculating strain of Kluyveromyces marxianus: bioreactor performance

  • Originals
  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

A flocculating strain of Kluyveromyces marxianus was used for alcoholic fermentation in a continuous bioreactor working with zero residual concentration in effluent. Specific kinetic parameters were improved by increasing dilution rate, which is similar to results obtained with ultrafiltration systems. Specific biomass accumulation rate had always a value greater than 92.5% of specific biomass growth rate and was independent of the dilution rate. Productivity is shown to be 12.5 times greater than in conventional continuous operation and is directly proportional to dilution rate. Maximum biomass concentration also presents a linear relationship with dilution rate. The largest obtained biomass concentration is 8 times greater than in a conventional continuous fermentor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

r p kg/(m3 · h):

ethanol production rate

r s kg/(kg · h):

lactose consumption rate

r x h−1 :

biomass growth rate

P kg/m3 :

ethanol concentration

Q m3/h:

volumetric flow rate

S kg/m3 :

lactose concentration

V dm3 :

volume

X kg/m3 :

biomass concentration

0 :

feed

1 :

recycle

2 :

fermentation zone outlet

3 :

global fermentor outlet

References

  1. Greenshields, R. N.; Smith, E. L.: Tower fermentation systems and their applications. The Chemical Engineer May (1971) 182–190

  2. Prince, I. G.; Barford, J. P.: Induced flocculation of yeasts for use in the tower fermentor. Biotechnology Letters 4 (1982) 621–626

    Google Scholar 

  3. Comberbach, D. M.; Bu'Lock, J. D.: Continuous ethanol production on the gas lift tower fermentor. Biotechnology Letters 6 (1984) 129–134

    CAS  Google Scholar 

  4. Netto, C. B.; Destruhaut, A.; Goma, G.: Ethanol fermentation by flucculating yeast: performance and stability dependance on a critical fermentation rate. Biotechnology Letters 7 (1985) 335–360

    Article  Google Scholar 

  5. Limtomg, S. A.; Nakata, M.; Funahashi, H.; Yoshida, T.; Siki, T.; Kumnuanta, J.; Taguchi, H.: Continuous ethanol production by a concentrated culture of flucculating yeast. J. Ferm. Technol. 62 (1984) 55–62

    Google Scholar 

  6. Admassu, W.; Korus, R. A.; Heimsch, R. C.: Ethanol fermentation with a flucculating yeast. The Chemical Engineering Journal 31 (1985) B1-B8

    Article  CAS  Google Scholar 

  7. Baratti, J; Varma, R.; Bu'Lock, J. D.: High productivity ethanol fermentation on a mineral medium using a flocculating strain of Zymomonas mobilis. Biotechnology Letters 8 (1986) 175–180

    Article  CAS  Google Scholar 

  8. Teixeira, J. A.; Mota, M.: Isolation of a flocculating strain of Kluyveromyces marxianus by a continuous method. Abstracts of the 4th European Conference: Biomass for Energy and Industry (1987) 273–274

  9. Delgenes, J. P.; Moletta, R.; Navarro, J. M.: Continuous production of ethanol from a glucose, xylose and arabinose mixture by a flocculent strain of Pichia stipitis. Biotechnology Letters 10 (1988) 725–730

    Article  CAS  Google Scholar 

  10. Chaplin, M. F.; Kennedy, J. F.: Carbohydrate analysis: a practical approach. Oxford: IRL Press 1986

    Google Scholar 

  11. Mota, M.: Inhibition et fermentation alcoholique: quelques concepts non conventionelles. Thesis. Toulouse: Inst. National des Sciences Appliquées 1985

    Google Scholar 

  12. Netto, C. B.: Fermentation alcoolique par levures floculées: étude des facteurs limitants et potentielites technologiques. Thesis. Toulouse: Inst. National des Sciences Apliquées 1984

    Google Scholar 

  13. Gerhardt, P.; Murray, R. G. E.; Costilow, R. N.; Nester, E. W.; Wood, W. A.; Krieg, N. R.; Phillips, G. B.: Manual of methods for general bacteriology. Washington: American Society for Microbiology 1981

    Google Scholar 

  14. Teixeira, J. A.; Mota, M.; Feyo de Azevedo, S.: Hydrodynamic and kinetic modelling of a flocculating bioreactor with cell recycle: Proceedings of the 4th International Congress on Computer Applications in Fermentation Technology. Cambridge: Society of Chemical Industry 1989. To be published

    Google Scholar 

  15. Aiba, S.; Shoda, M.; Nagatani, M.: Kinetics of product inhibition in alcohol fermentation. Biotechnol. Bioeng. 10 (1968) 845–865

    Article  CAS  Google Scholar 

  16. Mota, M.; Lafforgue, C.; Strehaiano, P.; Goma, G.: Fermentation coupled with microfiltration: kinetics of ethanol fermentation with cell recycle. Bioprocess Engineering 2 (1987) 65–68

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculdade de Engenharia, Centro de Engenharia Quimica da Universidade do Porto, Rua dos Bragas, 4099, Porto Codex, Portugal

    J. A. Teixeira & M. Mota

  2. Département de Génie Biochimique et Alimentaire, Institut National des Sciences Appliqées, Avenue de Rangueil, F-31077, Toulouse Cedex, France

    G. Goma

Authors
  1. J. A. Teixeira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Mota
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Goma
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Teixeira, J.A., Mota, M. & Goma, G. Continuous ethanol production by a flocculating strain of Kluyveromyces marxianus: bioreactor performance. Bioprocess Engineering 5, 123–127 (1990). https://doi.org/10.1007/BF00388191

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388191

Keywords

Search

Navigation