Abstract
Cell-free extracts of the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum strains 1C and L have been shown to cleave citrate with the formation of oxaloacetate and acetyl-CoA. This capacity was found in autotrophically grown cells as well as in the cells grown on media with acetate or L-glutamate. Citrate lyase activity in cell-free extracts is only measurable in the presence of citrate, adenosine-5′-triphosphate, coenzyme A and Mg2+ or Mn2+. It is concluded on the basis of the obtained data that C. limicola f. thiosulfatophilum contains adenosine-5′-triphosphate-linked citrate lyase (E.C.4.1.3.8). In contrast to green bacteria in the purple bacteria Ectothiorhodospira shaposhnikovii, Rhodospirillum rubrum and Thiocapsa roseopersicina citrate lyase activity was not found.
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References
Bergmeyer, H. U.: Methoden der enzymatischen Analyse, p. 403, Weinheim/Bergstraße: Verlag Chemie 1970
Beuscher, N., Gottschalk, G.: Lack of citrate lyase-the key enzyme of the reductive carboxylic acid cycle-in Chlorobium thiosulfatophilum and Rhodospirillum rubrum. Z. Naturforsch. 27b, 967–973 (1972)
Bogorov, L. V.: Properties of Thiocapsa roseopersicina strain BBS isolated from the estuary of the White sea. Microbiology 43, 326–332 (1974)
Bose, S. K., Gest, H., Ormerod, J. G.: Light-activated hydrogenase activity in a photosynthetic bacterium: a permeability phenomenon. J. Biol. Chem. 236, 13–14 (1961)
Evans, M. C. W., Buchanan, B. B., Arnon, D. I.: A new ferredoxindependent carbon reduction cycle in a photosynthetic bacterium. Proc. Natl. Acad. Sci. (Wash.) 55, 928–934 (1966)
Giffhorn, F., Beuscher, N., Gottschalk, G.: Regulation of citrate lyase activity in Rhodopseudomonas gelatinosa. Biochem. Biophys. Res. Commun. 49, 467–472 (1972)
Giffhorn, F., Gottschalk, G.: Effect of growth conditions on the activation and inactivation of citrate lyase of Rhodopseudomonas gelatinosa. J. Bacteriol. 124, 1046–1051 (1975a)
Giffhorn, F., Gottschalk, G.: Inactivation of citrate lyase from Rhodopseudomonas gelatinosa by a specific deacetylase and inhibition of this inactivation by L-(+)-glutamate. J. Bacteriol. 124, 1052–1061 (1975b)
Jones, M. E., Lipmann, F.: Aceto-CoA-kinase. In: Methods in enzymology, Vol. 1 (S. P. Colowick, N. O. Kaplan, eds). pp. 585–587. New York, London: Academic Press 1955
Kondratieva, E. N.: Photosynthetic Bacteria. London 1965 Oldbourne Press,
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Srere, P. A.: The enzymology of the formation and breakdown of citrate. Adv. Enzymol. 43, 57–101 (1975)
Virtanen, A. I., Mettinen, J. K., Kunttu, H.: α-keto-acids in green plants. Acta Chem. Scand. 7, 38–44 (1953)
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Ivanovsky, R.N., Sintsov, N.V. & Kondratieva, E.N. ATP-linked citrate lyase activity in the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum . Arch. Microbiol. 128, 239–241 (1980). https://doi.org/10.1007/BF00406165
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DOI: https://doi.org/10.1007/BF00406165