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Aerobic denitrification: a controversy revived

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Abstract

During studies on the denitrifying mixotroph, Thiosphaera pantotropha, it has been found that this organism is capable of simultaneously utilizing nitrate and oxygen as terminal electron acceptors in respiration. This phenomenon, termed aerobic denitrification, has been found in cultures maintained at dissolved oxygen concentrations up to 90% of air saturation.

The evidence for aerobic denitrification was obtained from a number of independant experiments. Denitrifying enzymes were present even in organisms growing aerobically without nitrate. Aerobic yields on acetate were higher (8.1 g protein/mol) without than with (6.0 g protein/mol) nitrate, while the anaerobic yield with nitrate was even lower (4 g protein/mol). The maximum specific growth rate of Tsa. pantotropha was higher (0.34 h-1) in the presence of both oxygen (>80% air saturation) and nitrate than in similar cultures not supplied with nitrate (0.27 h-1), indicating that the rate of electron transport to oxygen was limiting. This was confirmed by oxygen uptake experiments which showed that although the rate of respiration on acetate was not affected by nitrate, the total oxygen uptake was reduced in its presence. The original oxygen uptake could be restored by the addition of denitrification inhibitors.

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References

  • Goa J (1953) A microbiuret method for protein determination; determination of total protein in cerebrospinal fluid. J Clin Lab Invest 5:218–222

    PubMed  Google Scholar 

  • Griess-Romijn-van Eck (1966) Physiological and chemical tests for drinking water. NEN 1056, IV-2 Nederlands Normahsatie Instituut Rijswijk

  • Harrison AP (1983) Genomic and physiological comparisons between heterotrophic Thiobacilli and Acidiphillium cryptum, Thiobacillus versutus sp. nov., and Thiobacillus acidiphilium nom. rev. Int J Syst Bacteriol 33:211–217

    Google Scholar 

  • Krul JM (1976) Dissimilatory nitrate and nitrite reduction under aerobic conditions by an aerobically and anaerobically grown Alcaligines sp. and by active sludge. J Appl Bacteriol 40:245–260

    PubMed  Google Scholar 

  • Krul JM, Veenningen R (1977) The synthesis of the dissimilatory nitrate reductase under aerobic conditions in a number of denitrifying bacteria isolated from active sludge and drinking water. Water Res 11:39–43

    Google Scholar 

  • Marshall RO, Dishburger HJ, MacVicar R, Hallmark GD (1953) Studies on the effect of aeration on nitrate reduction by Pseudomonas species using N15. J Bacteriol 66:254–258

    PubMed  Google Scholar 

  • Meiberg JBM, Bruinenberg PM, Harder W (1980) Effect of dissolved oxygen tension on the metabolism of methylated amines in Hyphomicrobium X in the absence and presence of nitrate: evidence for “aerobic” denitrification. J Gen Microbiol 120:453–463

    Google Scholar 

  • Meschner K von, Wuhrmann K (1963) Beitrag zur Kenntnis der microbiellen Denitrification. Pathol Microbiol 26:579–591

    Google Scholar 

  • Ottow JCG, Fabig W (1963) Influence of oxygen aeration on denitrification and redox level in different bacterial batch cultures. Abstracts of the Proceedings of the 6th International Syposium on Environmental Biogeochemistry, p 61

  • Payne WJ (1981) In: Denitrification. John Wiley & Sons, New York, pp 61–65

    Google Scholar 

  • Robertson LA, Kuenen JG (1983) Thiosphaera pantotropha gen. nov. sp. nov., a facultatively anaerobic, facultatively autotrophic sulphur bacterium. J Gen Microbiol 129:2847–2855

    Google Scholar 

  • Sawhney V, Nicholas DJD (1977) Sulfite and NADH dependant nitrate reductase from Thiobacillus denitrificans. J Gen Microbiol 100:49–58

    Google Scholar 

  • Stouthamer AH (1980) Bioenergetic studies on Paracoccus denitrificans. Trends in Biochemical Sciences 5:164–166

    Article  Google Scholar 

  • Taylor BF, Hoare DS (1969) New facultative Thiobacillus and a reevaluation of the heterotrophic potential of Thiobacillus novellus. J Bacteriol 100:487–497

    PubMed  Google Scholar 

  • Tiedje JM, Sexstone AJ, Myrold DD, Robinson JA (1982) Denitrification; ecological niches, competition and survival. Antonic van Leeuwenhoek 48:529–607

    Google Scholar 

  • Timmer ten Hoor A (1977) In: Denitrificerende kleurloze bacterien. PhD thesis, University of Groningen

  • Vishniac W, Santer M (1957) The Thiobacilli. Bacteriol Rev 21:195–213

    PubMed  Google Scholar 

  • Watahiki M, Hata S, Aida T (1983) N2O accumulation and inhibition of N2O reduction by denitrifying Pseudomonas sp. 220A in the presence of oxygen. Agric Biol Chem 47:1991–1996

    Google Scholar 

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Dedicated to Professor Dr. H.-G. Schlegel on the occasion of his 60th birthday

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Robertson, L.A., Kuenen, J.G. Aerobic denitrification: a controversy revived. Arch. Microbiol. 139, 351–354 (1984). https://doi.org/10.1007/BF00408378

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  • DOI: https://doi.org/10.1007/BF00408378

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