Summary
Mutants of Saccharomyces cerevisiae with enhanced sensitivity to the DNA cross-linking agent nitrogen mustard (HN2) have been isolated and partially characterized with respect to their phenotypic and genetic properties. The screening technique, based on HN2-sensitivity as sole criterion, yields approximately 1 sensitive isolate in 200 clones when applied to an intensively mutagenized population of a resistant parent strain. Mutants characterized so far are all due to recessive nuclear genes and represent at least seven complementation groups. They exhibit different degrees as well as different patterns of sensitivity towards monofunctional and bifunctional alkylating agents, and ultraviolet light.
Similar content being viewed by others
References
Brendel M, Haynes RH (1973) Interactions among genes controlling sensitivity to radiation and alkylation in yeast. Mol Gen Genet 125:197–216
Brendel M, Khan N, Haynes RH (1970) Common steps in the repair of alkylation and radiation damage in yeast. Mol Gen Genet 106:289–295
Correia JS, Tyrrell RM (1979) Lethal interactions between ultravioletviolet radiations and methyl methanesulphonate in repair proficient and repair deficient strains of Escherichia coli. Photochem Photobiol 29:521–526
Cox BS, Parry JM (1968) The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutat Res 6:37–55
Fleer R, Brendel M (1979) Formation and fate of cross-links induced by polyfunctional anticancer drugs in yeast. Mol Gen Genet 176:41–52
Gocke E, Kiefer J (1977) Synthesis of inducible enzymes in irradiated yeast cells. Biochim Biophys Acta 479:207–213
Haynes RH, Prakash L, Resnick MA, Cox BS, Moustacchi E, Boyd JB (1978) DNA repair in lower eucaryotes. In: Hanawalt PC, Friedberg EC, Fox FF (eds) DNA repair mechanisms. Academic Press Inc, New York, p 405
Kircher M, Fleer R, Ruhland A, Brendel M (1979) Biological and chemical effects of mustard gas in yeast. Mutat Res 63:273–289
Knauert FK (1977) The isolation and characterization of nitrous acid sensitive (nas) mutants of the yeast Sacharomyces cerevisiae. PhD Thesis, U California Davis
Mortimer RK, Hawthorne DC (1975) Genetic mapping in yeast. In: Prescott DM (ed) Methods in cell biology, vol XI. Academic Press Inc, New York, p 221
Prakash L (1974) Lack of chemically induced mutation in repairdeficient mutants of yeast. Genetics 78:1101–1118
Prakash L (1976) Effect of genes controlling radiation sensitivity on chemically induced mutations in Saccharomyces cerevisiae. Genetics 83:285–301
Prakash L, Prakash S (1977) Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics 86:33–55
Ruhland A, Brendel M (1979) Mutagenesis by cytostatic alkylating agents in yeast strains of differing repair capacities. Genetics 92:83–97
Ruhland A, Fleer R, Brendel M (1978) Genetic activity of chemicals in yeast: DNA alterations and mutations induced by alkylating anti-cancer agents. Mutat Res 58:241–250
Scudiero DA, Friesen BS, Baptist JE (1973) Physiological modifications in the production and repair of methyl methanesulfonate-induced breaks in the deoxyribonucleic acid of Escherichia coli K12. J Bact 114:267–272
Shneyour Y, Stamberg J, Hundert P, Werczberger R, Koltin Y (1978) Selection with cycloheximide of metabolic and UV-sensitive mutants of Schizophyllum commune and Saccharomyces cerevisiae. Mutat Res 49:195–201
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Ruhland, A., Haase, E., Siede, W. et al. Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard. Molec. Gen. Genet. 181, 346–351 (1981). https://doi.org/10.1007/BF00425609
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425609