Summary
Barley β-glucans present in wort reduce beer filtrability and cause hazes and precipitates in the finished beer. The endo-β-1,4-glucanase enzyme, EGI, found in the filamentous fungus Trichoderma reesei, is capable of efficiently hydrolyzing these β-glucans. The cDNA copy of the eg11 gene, which codes for the EGI enzyme, was coupled to yeast regulatory sequences and transferred to a brewer's yeast using the yeast copper chelatin gene CUP1 as a selection marker in the transformation. The eg11 gene was transferred to the yeast both on a multicopy plasmid and on an integrating plasmid. In both cases, highly glycosylated, active EGI enzyme was secreted into the medium. Barley β-glucans present in wort were efficiently hydrolyzed by the recombinant brewer's yeast.
Similar content being viewed by others
References
Analytica-EBC (1975) Schweizer Brauerei-Rundschau, Zürich, E86/21-E86/22
Andreadis A, Hsu Y-P, Hermodson M, Kohlkaw G, Schimmel P (1984) J Biol Chem 259:8059–8062
Arsdell JNv, Kwok S, Schweickart VL, Ladner MB, Gelfand DH, Innis MA (1987) Bio/Technology 5:60–64
Broach JR (1981) In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces, life cycle and inheritance. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 445–470
Cantwell B, Brazil G, Hurley J, McConnell D (1985) Proc of the 20th Internat Cong Eur Brewery Convention. Helsinki 1985, pp 259–266
Enari T-M, Niku-Paavola M-L (1987) CRC Critical Rev in Biotechnol 5:67–87
Erdal K, Gjertsen P (1968) Proc 11th Internat Cong Eur Brewery Convention. Madrid 1967, pp 295–302
Harashima S, Takagi A, Oshima Y (1984) Mol Cell Biol 4:771–778
Henderson RCA, Cox BS, Tubb R (1985) Curr Genet 9:133–138
Hinchliffe E, Box WG (1985) Proc 20th Internat Cong Eur Brewery Convention. Helsinki 1985, pp 267–274
Hinchliffe E, Daubney CJ (1986) Am Soc Brewing Chem 44:98–101
Holm C, Meeks-Wagner DW, Fangman WL, Botstein D (1986) Gene 42:169–173
Jackson JA, Fink GR (1981) Nature 292:306–311
Klein HL, Petes TD (1981) Nature 289:144–148
Kielland-Brandt MC, Nilsson-Tillgren T, Litske Petersen JG, Holmberg S, Gjermansen C (1983) in: Spencer JFT, Spencer DM, Smith ARW (eds) Yeast genetics. Fundamental and applied aspects. Springer, NY, pp 421–437
Kingsman SM, Dobson MJ, Tuite MS, Mellor J, Roberts NA, Kingsman AJ (1983) In: Korhola M, Väisänen E (eds) Gene expression in yeast. Proc Alko Yeast Symposium. Helsinki, Finland, 1983, pp 95–114
Knowles JKC, Tubb R (1987) EBC Symposium on Brewer's Yeast. Helsinki 1986, Monograph XII, pp 169–185
Knowles JKC, Penttilä M, Teeri TT, André L, Salovuori I, Lehtovaara P (1985) Proc 20th Internat Cong Brewery Convention. Helsinki 1985, pp 251–258
Knowles JKC, Lehtinen U, Nikkola M, Penttilä M, Suihko M-L, Home S, Vilpola A, Enari T-M (1987) Proc 21st Internat Cong Eur Brewery Convention. Madrid 1987 (in press)
Lehtovaara P, Knowles JKC, André L, Penttilä M, Teeri TT, Salovuori I, Niku-Paavola M-L, Enari T-M (1986) 3rd Internat Conf Biotechnol Pulp and Paper Industry. Stockholm 1986, pp 92–92
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Springer Harbor Laboratory, Cold Springer Harbor, NY
Meaden PG, Tubb RS (1985) Proc 20th Internat Cong Eur Brewery Convention. Helsinki 1985, pp 219–229
Mellor J, Dobson MJ, Roberts NA, Tuite MF, Emtage JS, White S, Lowe PA, Patel T, Kingsman AJ, Kingsman SM (1983) Gene 24:1–14
Niku-Paavola M-L, Lappalainen A, Enari T-M, Nummi M (1985) Biochem J 231:75–81
Orr-Weaver TL, Szostak JW, Rothstein RJ (1981) Proc Natl Acad Sci USA 78:6354–6358
Orr-Weaver TL, Szostak JW, Rothstein RJ (1983) Meth Enzymol 101:228–245
Penttilä M, Lehtovaara P, Nevalainen H, Bhikhabhai R, Knowles J (1986) Gene 45:253–263
Penttilä M, André L, Saloheimo M, Lehtovaara P, Knowles JKC (1987) Yeast 3 (in press)
Rothstein R (1985) In: Glover DM (ed) DNA cloning II, a practical approach. IRL Press Limited, Oxford, pp 45–66
Rudolph H, Rauseo-Koenig I, Hinnen A (1985) Gene 36:87–95
Szostak JW, Wu R (1979) Plasmid 2:536
Tilbeurgh H van, Claeyssens M (1985) Febs Lett 187:283–288
Tubb RS (1980) J Inst Brew 86:78–80
Yocum RR (1986) Proc Bio Expo 86. Butterworth, Stoneham MA, pp 171–180
Yanish-Perron C, Vieira J, Messing J (1985) Gene 33:103–119
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Penttilä, M.E., Suihko, M.L., Lehtinen, U. et al. Construction of brewer's yeasts secreting fungal endo-ß-glucanase. Curr Genet 12, 413–420 (1987). https://doi.org/10.1007/BF00434818
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00434818