Abstract
An unusual filamentous, budding bacterium was isolated from several North American hot springs and named Isosphaera pallida. Filaments are composed of spherical cells 2.5–3.0 μm in diameter, with cell growth and division occurring by formation of intercalary buds. These obligately aerobic, heterotrophic isolates closely resemble Isocystis pallida Woronichin, which has been previously described as a cyanobacterium, and later as a yeast, based on collected specimens.
Isolates were salmon-colored due to the presence of carotenoids and contained gas vesicles. Growth occurred at temperatures up to 55° C in defined media using 0.025% glucose or lactate as carbon sources. Glucose concentrations of 0.05% or higher inhibited growth of the culture.
Ultrathin sections observed by TEM revealed an unusual tri-laminar wall structure. Pit-like ultrastructural features were found in the cell wall. Growth of cultures was not inhibited by penicillin G, and the Gram reaction gave variable results.
Cells formed motile, macroscopic aggregates (“comets”) when harvested from liquid cultures and plated on media containing Gelrite (Kelco Co.) as a solidifying agent. Aggregation and motility were observed in both the light and the dark. However, comets were strongly phototactic. Negative stains revealed numerous pili, but not flagella.
We propose that this highly unusual prokaryote be placed in a new genus.
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References
Anagnostidis K, Rathsack-Kuzenbach R (1967) Isocystis pallida-Blaualgae oder hefeartiger Pilz. Schweiz Zeit Hydrol 29:191–198
Barbour AG, Amano KI, Hackstadt T, Perry L, Caldwell HD (1982) Chlamydia trachomatis has penicillin-binding proteins but not detectable muramic acid. J Bacteriol 151:420–428
Bornet E, Flahault C (1888) (reprinted 1959). Revision des Nostocacées hétérocystées. Ann Sci Nat Bot 7:178–262
Bradfield AR (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Castenholz RW (1982) Motility and taxes. In: Carr NG, Whitton BA (eds) The biology of cyanobacteria. Blackwell. Oxford and Univ of Calif, Berkeley, pp 413–439
Elenkin AA (1949) Sineselenije wodorosei SSSR (Monographia algarum Cyanophycearum aquidulcium et terrestrium in finibus URSS inventarum pars specialis. Akad Nauk, Moskwa Leningrad, Fasc 2:985–1908
Garrett AJ, Harrison MJ, Manire GP (1974) A search for the bacterial mucopeptide component muramic acid in Chlamydia. J Gen Microbiol 80:315–318
Gebers R, Wehmeyer U, Roggentin T, Schlesner H, Kolbel-Boelke J, Hirsch P (1985) Deoxyribonucleic acid base compositions and nucleotide distributions of 65 strains of budding bacteria. Int J Sys Bacteriol 35:260–269
Geitler L (1932) Cyanophyceae. In: Rabenhorst L (ed) Kryptogamen-Flora von Deutschland, Österreich und der Schweiz, vol XIV. Akademische Verlagsbuchhandlung, Leipzig
Geitler L (1955) Torutopsidosira n. gen., ein neuer hefeartiger Pilz, und andere knospende Mikroorganismen. Archiv Mikrobiol 22:324–334
Gettler L (1963) Die angebliche Cyanophycee Isocystis pallida ist ein hefeartiger Pilz. Arch Mikrobiol 46:238–242
König E, Schlesner H, Hirsch P (1984) Cell wall studies on budding bacteria of the Planctomyces/Pasteuria group and on a Prosthecomicrobium sp. Arch Microbiol 138:200–205
Macnab R, Koshland DE (1974) Bacterial motility and chemotaxis: light induced tumbling response and visualization of individual flagella. J Mol Biol 84:399–406
Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–219
Nelson D, Castenholz R (1982) Light responses in Beggiatoa. Arch Microbiol 131:146–155
Pfennig N, Trüper HG (1981) Isolation of members of the Chromatiaceae and Chlorobiaceae. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG (eds) The prokaryotes. A handbook on habitats, isolation, and identification of bacteria. Springer, Berlin Heidelberg New York, pp 279–289
Schmidt JM, Starr MP (1981) The Blastocaulis-Planctomyces group of buddding and appendaged bacteria. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. A handbook on the habitats, isolation, and identification of bacteria. Springer, Berlin Heidelberg New York, pp 496–504
Schmidt JM, Starr MP (1982) Ultrastructura features of budding cells in a prokaryote belonging to morphotype IV of the Blastocaulis-Planctomyces group. Curr Microbiol 7:7–11
Stackebrandt E, Ludwig W, Schubert W, Klink F, Schlesner H, Roggentin T, Hirsch P (1984) Molecular genetic evidence of early evolutionary origin of budding peptidoglycan-less eubacteria. Nature 307:735–737
Starmach K (1966) Cyanophyta-Glaucophyta (Sinice-Glaukofity). Flora slodkowodna Polski, vol 2. Polska Akad Nauk, Warsaw (in Polish), pp 1–807
Taylor BL, Koshland DE (1975) Intrinsic and extrinsic light responses in Salmonella typhimurium and Escherichia coli. J Bacteriol 123:557–569
Woronichin NN (1927) Materiali k agologitscheskoj flore i rastitjelnosti mineralnich istotschnikov gruppi Kaukaskich mineralnich wod. Trav Inst Balneol aux Eaux Miner du Caucase 5:90–121
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Giovannoni, S.J., Schabtach, E. & Castenholz, R.W. Isosphaera pallida, gen. and comb. nov., a gliding, budding eubacterium from hot springs. Arch. Microbiol. 147, 276–284 (1987). https://doi.org/10.1007/BF00463488
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DOI: https://doi.org/10.1007/BF00463488