Abstract
Cell envelopes were prepared from freeze-dried cells of 8 strains of budding bacteria belonging to the Pirella/Planctomyces group. Treatment with 10% sodium dodecylsulfate (SDS) (30 min, 100°C) allowed the isolation of stable cell sacculi which still maintained the original cell shape. The chemical analysis showed, as the main component, protein which was unusually rich in proline and cystine. Except for Planctomyces maris IFAM 1317 (where this protein comprised 62.6% of the total envelope dry weight) the corresponding values for the other strains varied from 75 to 82%. Amino sugars and neutral sugars were present only in small amounts and uronic acids were not found. The ash content varied from 5 to 10% of the dry weight, except for IFAM 1317 which had 19% ash. The high content of cystine indicated a high degree of crosslinking of the cell envelopes through disulfide bonds. Our data show that bacteria of the Pirella/Planctomyces group possess a similar cell wall composition.
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References
Albersheim P, Nevins DJ, English PD, Karr A (1967) A method for the analysis of sugars in plant-wall polysacharides by gas liquid chromatography. Carbohydr Res 5:340–345
Bailey JL (1962) Techniques in protein chemistry. Elsevier-Publishing Company, Amsterdam London New York
Bergmeyer HU (1984) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim
Galambos JT (1967) The reaction of carbazole with carbohydrates. I. Effect of borate and sulfomate on the carbazole colour of sugars. Anal Biochem 19:119–132
Hirsch P, Müller M (1985) Planctomyces limnophilus sp., nov., a stalked and budding bacterium from freshwater. System Appl Microbiol 6:276–280
König H, Schlesner H, Hirsch P (1984) Cell wall studies on budding bacteria of the Planctomyces/Pasteuria group and on a Prosthecomicrobium sp., Arch Microbiol 138:200–205
Reynolds ES (1963) The use of lead citrate at high pH as an electronopaques stain in electron microscopy. J Cell Biol 17:208–212
Schlesner H, Hirsch P (1984) Assignment of ATCC 27377 to Pirella staleyi gen. and spec. nov. on the basis of pure culture laboratory studies, and comments on a request to assign this strain to the genus Planctomyces. Int J System Bacteriol 34:492–495
Schmidt J, Starr MP (1978) Morphological diversity of fresh-water bacteria belonging to the Blastocaulis-Planctomyces group as observed in natural populations and enrichments. Current Microbiol 1:325–330
Schmidt J, Starr MP (1979) Morphotype V of the Blastocaulis-Planctomyces groups of budding and appendaged bacteria: Planctomyces guttaeformis Hortobagyi (sensu Hajdu). Current Microbiol 2:195–200
Spurr A (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastr Res 26:31–43
Stackebrandt E, Ludwig W, Schubert W, Klink F, Schlesner H, Roggentin T, Hirsch P (1984) Molecular genetic evidence for early evolutionary origin of budding peptidoglycanless eubacteria. Nature 307:735–737
Stackebrandt E, Fischer A, Hirsch P, Roggentin T, Schlesner H (1986) The phylogeny of an ancient group of budding peptidoglycanless eubacteria: the genera Planctomyces and Pirella. Endocyt C Res 3:29–40
Woese CR, Maniloff J, Zablen LB (1980) Phylogenetic analysis of the Mycoplasmas. Proc Natl Acad Sci (USA) 77:494–498
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Liesack, W., König, H., Schlesner, H. et al. Chemical composition of the peptidoglycan-free cell envelopes of budding bacteria of the Pirella/Planctomyces group. Arch. Microbiol. 145, 361–366 (1986). https://doi.org/10.1007/BF00470872
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DOI: https://doi.org/10.1007/BF00470872