Abstract
Allozyme variability was analyzed at 16 loci in 11 lines of Anopheles stephensi Liston from Pakistan. Six lines were considered as samples from natural populations. For these lines the mean number of alleles was 1.31–1.63, the degree of polymorphism was 0.188–0.375, the observed heterozygosity was 0.065–0.086, and the genetic distance ranged from 0.001 to 0.016. No population-specific alleles were found. Interbreeding was considerable (mean F it=0.183). Differences in allele frequencies were due primarily to local inbreeding (F is>F st at most loci). The Lahore line, reared for more than 20 generations, had more homozygotes than the other lines. A line refractory to Plasmodium falciparum and a genetic sexing line exhibited decreased allozyme variability. The latter line showed reduced staining intensity at 10 loci. Linkage studies are recommended for the following loci with rare alleles: Acp, Gapdh, Icd-1, Icd-2, Mpi, and Pgd.
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This research was financially supported in part by the Dutch Ministries of Agriculture & Fisheries, Developmental Aid, and Science Policy. It is part of the collaborative program INAL between the Research Institute ITAL, Wageningen, and the Departments of Parasitology of the Universities of Nijmegen, Amsterdam, and Leiden.
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van Driel, J.W., Sluiters, J.F. & van der Kaay, H.J. Allozyme variation in Anopheles stephensi Liston from Pakistan (Diptera: Culicidae). Biochem Genet 25, 789–802 (1987). https://doi.org/10.1007/BF00502599
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DOI: https://doi.org/10.1007/BF00502599