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Multistage production of Autographa californica nuclear polyhedrosis virus in insect cell cultures

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Abstract

The aim of our study was to establish an efficient system for thein vitro production of the insect pathogenic Autographa californica nuclear polyhedrosis virus in a Spodoptera frugiperda cell line. We optimized cultivation conditions for cell proliferation as well as for virus replication in a 1.5 litre stirred tank bioreactor. Cell and virus propagation were found to be optimal at a constant oxygen tension of 40%. In order to provide sufficient nutrients during virus synthesis filtration and perfusion devices were connected to the bioreactor. A virus production procedure in a repeated batch mode by using a two stage bioreactor system is described. Stage I was optimized for cell production and stage II for virus production.

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Abbreviations

Ac-NPV:

Autographa californica Nuclear Polyhedrosis Virus

BV:

Baculovirus

MOI:

Multiplicity Of Infection

ECV:

Extracellular Virus

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Klöppinger, M., Fertig, G., Fraune, E. et al. Multistage production of Autographa californica nuclear polyhedrosis virus in insect cell cultures. Cytotechnology 4, 271–278 (1990). https://doi.org/10.1007/BF00563787

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  • DOI: https://doi.org/10.1007/BF00563787

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