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PDR3, a new yeast regulatory gene, is homologous toPDR1 and controls the multidrug resistance phenomenon

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Abstract

TheSaccharomyces cerevisiae PDR3 gene, located near the centromere of chromosome II, has been completely sequenced and characterised. Mutationspdr3-1 andpdr3-2, which confer resistance to several antibiotics can be complemented by a wild-type allele of the PDR3 gene. The sequence of the wild-typePDR3 gene revealed the presence of a long open reading frame capable of encoding a 976-amino acid protein. The protein contains a single Zn(II)2Cys6 binuclear-type zinc finger homologous to the DNA-binding motifs of other transcriptional activators from lower eukaryotes. Evidence that the PDR3 protein is a transcriptional activator was provided by demonstrating that DNA-bound LexA-PDR3 fusion proteins stimulate expression of a nearby promoter containing LexA binding sites. The use of LexA-PDR3 fusions revealed that the protein contains two activation domains, one localised near the N-terminal, cysteine-rich domain and the other localised at the C-terminus. The salient feature of the PDR3 protein is its similarity to the protein coded byPDR1, a gene responsible forpleiotropicdrugresistance. The two proteins show 36% amino acid identity over their entire length and their zinc finger DNA-binding domains are highly conserved. The fact that the absence of both PDR1 and PDR3 (simultaneous disruption of the two genes) enhances multidrug sensitivity strongly suggests that the two transcriptional factors have closely related functions.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire, CNRS URA 1302, Ecole Normale Supérieure 46 rue d'Ulm, 75230, Paris Cedex 05, France

    Thierry Delaveau, Agnès Delahodde & Claude Jacq

  2. Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynska dolina B2, 842 15, Bratislava, Slovak Republic

    Julius Subik

  3. Unite de Biochimie Physiologique, Université de Louvain, Place Croix du Sud, 2-20, B-1348, Louvain-La-Neuve, Belgium

    Elvira Carvajal

Authors
  1. Thierry Delaveau
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  2. Agnès Delahodde
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  3. Elvira Carvajal
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  4. Julius Subik
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  5. Claude Jacq
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Communicated by C.P. Hollenberg

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Delaveau, T., Delahodde, A., Carvajal, E. et al. PDR3, a new yeast regulatory gene, is homologous toPDR1 and controls the multidrug resistance phenomenon. Molec. Gen. Genet. 244, 501–511 (1994). https://doi.org/10.1007/BF00583901

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  • DOI: https://doi.org/10.1007/BF00583901

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