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Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle

  • Excitable Tissues and Central Nervous Physiology
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Abstract

To study the stimulating effect of adrenaline (ADR) on active Na+/K+ transport we used double-barrelled ion-sensitive micro-electrodes to measure the activities of extracellular K+ (aKe) and intracellular Na+ (aNai) in isolated preparations of rat soleus muscle, normal human intercostal muscle and one case of hyperkalemic periodic paralysis (h.p.p.). In these preparations bath-application of ADR (10−6 M) resulted in a membrane hyperpolarization and transient decreasesaKe andaNai which could be blocked by ouabain (3×10−4 M). In the h.p.p. muslce a continuous rise ofaNai induced by elevation ofaKe to 5.2 mM could be stopped by ADR. In addition, the intracellular K+ activity (aKi), the free intracellular Ca2+ concentration (pCai) and intracellular pH (pHi) were monitored in rat soleus muscle. During ADRaKi increased, pHi remained constant and intracellular Ca2+ apparently decreased. In conclusion, our data show that ADR primarily stimulates the Na+/K+ pump in mammalian skeletal muscle. This stimulating action is not impaired in the h.p.p. muscle.

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Parts of the results have been presented to the German Physiological Society (Ballanyi and Grafe 1987)

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Ballanyi, K., Grafe, P. Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle. Pflugers Arch. 411, 283–288 (1988). https://doi.org/10.1007/BF00585116

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  • DOI: https://doi.org/10.1007/BF00585116

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