Summary
Primary tissue cultures of human gliomas were treated with cytochalasin B (0.5–60 μg/ml for 90 min). Cell motility was inhibited irreversibly in glial tumour cells, but the effect was reversible on the mesenchymal cells growing in culture in the lower dose range. Cell adhesion was considerably reduced as the dose was increased, as was the capacity for cells to spread on a surface from suspension. Low concentrations of cytochalasin B caused negligible cell death and little disruption of cell ultrastructure. However, increases in dose were accompanied by a greater predominance of rough endoplasmic reticulum and inclusions and aggregation of microfilament bundles. As seen by scanning electron microscopy, cytochalasin B caused the withdrawal of peripheral cell borders, disappearance of ruffles and the breakdown of cytoplasmic lamellae. Charateristic surface blebs and folds appeared in their place.
By comparison, colchicine (1–10 μg/ml) caused a less marked and non-specific reversible reduction in cell motility on both glial and mesenchymal cells. No significant change in cell adhesion or spreading took place even at high doses, although at all concentrations gross disruption of the cell surface took place with changes in ultrastructure characterised by loss of cytoplasmic microtubules and aggregation of 10 nm filaments.
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Allison, A. C.: The role of microfilaments and microtubules in cell movement, endocytosis and exocytosis. In: Locomotion of tissue cells. Ciba Foundation Symposium. 4. New York-Amsterdam: Elsevier, North-Holland 1973
Barker, M., Hoshino, T., Guracy, O., Wilson, C. B., Nielsen, S. L., Downie, R., Eliason, J.: Development of an animal brain tumour model and its response to therapy with 1,3-bis(2-chloroethyl)-l-nitrosourea. Cancer Res.33, 976–986 (1973)
Carter, S. B.: Effects of cytochalasins on mammal cells. Nature213, 261–264 (1967)
Chen, T. T., Mealey, J.: Effects on cytosine arabinoside and 1,3-bis(2-chloroethyl)-l-nitrosourea on human glial tumour cells. Cancer Res.32, 558–564 (1972)
Cobb, J. P., Walker, D. G., Wright, J. C.: Comparative chemotherapeutic studies on primary short-term cultures of human normal, benign and malignant tumour tissues—a five-year study. Cancer Res.21, 583–590 (1961)
Croop, J., Holzer, M.: Response of mygenic and fibrogenic cells to cytochalasin B and to colcemid. J. Cell Biol.65, 271–285 (1975)
Donoso, J. A., Green, L. S., Heller-Bettinger, I. E., Samson, F. E.: Action of the vinca alkaloids, vincristine, vinblastine and desacetyl vinblastine amide on axonal fibrillar organelles in vitro. Cancer Res.37, 1401–1407 (1977)
Easty, D. M., Wylie, J. A. H.: Sereening of 12 gliomata against chemotherapeutic agents in vitro. Br. Med. J.141, 1589–1592. (1963)
Grasso, R. J., Moore, N. A., Boler, R. K., Johnson, C. E.: Increased volume of mitochondria and granular endoplasmic reticulum in rat glioma cells treated with anti-tumour agents. Proc. Soc. Exp. Biol. Med.155, 219–224 (1977)
Greenaway, J. C., Shepard, T. H., Kuc, J.: Comparison of cytochalasins (A, B, D and E) in chick explant teratogenicity and tissue culture systems. Proc. Soc. Exp. Biol. Med.155, 232–242 (1977)
Groscel-Stewart, U., Unsicker, H., Leonhardt, H.: Immunohistochemical demonstration of contractile proteins in astrocytes, marginal glial and ependymal cells in rat diencephalon. Cell. Tissue. Res.180, 133–137 (1977)
Hagmar, B., Ryd, W.: Tumour cell locomotion—a factor in metastasis formation? Influence of cytochalasin B on dissemination pattern. Int. J. Cancer19, 576–580 (1977)
Haynes, L. W., Davies, B. E., Mitchell, J., Weller, R. O.: Cell proliferation in explant cultures of malignant gliomas. Acta Neuropathol. (Berl.) (in press) (1978)
Haynes, L. W., Weller, R. O.: A comparison of some features of glial differentiation with dibutyryl cyclic AMP-induced changes in human glioma cells in culture. Br. J. Exp. Pathol. (in press) (1978)
Hoshino, T., Wilson, C. B.: Review of basic concepts of cell kinetics as applied to brain tumours. J. Neurosurg.42, 123–131 (1975)
Kurki, P., Linder, E., Virtanen, I., Stenman, S.: Human smooth muscle auto-antibodies reacting with intermediate (100⫗A) filaments. Nature268, 240–241 (1977)
Mealey, J., Chen, T. T., Shupe, R.: Response of cultured human glioblastomas to radiation and BCNU chemotherapy. J. Neurosurg.41, 339–349 (1974)
Murray, M. R., Peterson, E. R., Hirschberg, E., Pool, J. L.: Metabolic and chemotherapeutic investigation of human glioblastoma in vitro. Ann. N.Y. Acad. Sci.58, 1147–1171 (1956)
Posner, J. P., Shapiro, W. R.: Brain tumour. Current status of treatment and its complications. Arch. Neurol.32, 781–794 (1975)
Sanger, J. W., Holzer, H.: Cytochalasin B: Effects on cell morphology, cell adhesion and mucopolysaccharide synthesis. Proc. Nat. Acad. Sci. U.S.A.69, 253–257 (1973)
Sorour, O., Mostafa, R., Nabil, E., Mohamed, R.: Infiltrative potentiality of brain tumours in organ culture. J. Neurosurg.43, 742–749 (1975)
Tannenbaum, J., Tannenbaum, S. W., Goodman, G. C.: The binding sites of cytochalasin D. II: Their relationship to hexose transport and cytochalasin B. J. Cell Biol.91, 239–248 (1977)
Weber, K., Rathke, P. C., Osborne, M. Franke, W. W.: Distributation of actin and tubulin in cells and in glycerinated cell models after treatment with cytochalasin B. Exp. Cell Res.102, 285–297 (1976)
Wessels, N. K., Spooner, B. S., Ludena, M. A. Surface movements, microfilaments and cell locomotion. In: Locomotion of tissue cells. Ciba Foundation Symposium 14. New York-Amsterdam: Elsevier, North-Holland 1973
Wheeler, K. T., Teb, N., Williams, M. E., Sheppard, S., Levin, V. A., Kabra, P. M.: Factors influencing the survival of rat brain tumour cells after in vitro treatment with 1,3-bis(2-chloroethyl)-l-nitrosourea on human glial tumour cells. Cancer Res35, 1464–1469 (1975)
Wilson, C. B., Barker, M.: Sensitivity of cell cultures of neural tumours to vinblastine sulphate (NSC-49842). Cancer Chemother. Rep.44, 9–13 (1965)
Zimmerman, H. M.: The significance of experimental gliomas for human disease. In: Gliomas. Current concepts in biology, diagnosis and therapy. Recent Results in Cancer Research. Symp. 51. Berlin-Heidelberg-New York: Springer 1975
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Haynes, L.W., Weller, R.O. The effects of cytochalasin B and colchicine on cell motility and ultrastructure in primary cultures of malignant gliomas. Acta Neuropathol 44, 21–30 (1978). https://doi.org/10.1007/BF00691635
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DOI: https://doi.org/10.1007/BF00691635