Summary
Pectoralis muscles from normal and dystrophic chickens were investigated 2h after an i.v. injection of horseradish peroxidase, by cytochemical and biochemical techniques to demonstrate peroxidase activity.
Light microscopic examination of dystrophic museles showed that peroxidase activity could be detected inside a population of fibers, in deliminated bodies often restricted to segments of the muscle fiber. Such bodies containing peroxidase were not observed in normal muscle fibers. Electron microscopy of dystrophic muscle fibers revealed that numerous vesicles containing peroxidase were frequently present in fiber regions with signs of cytoplasmic degradation. These vesicles, which occasionally were found to be coated, were 50–100 nm in size and appeared to be derived from t-tubules. Larger (up to 1.7 μm) inclusions containing peroxidase and delimited by a single membrane were also present at degenerating areas of dystrophic muscle fibers. These bodies seemed to be formed by fusion between several primary t-tubule vesicles and probably also lysosomes. Vacuoles containing the peroxidase were frequently encountered. Biochemical determination of horseradish peroxidase activity, performed after extensive washing of the muscle tissue, showed that dystrophic muscles contained about twice as much peroxidase as normal control muscles.
It is suggested that endocytosis from t-tubules is an early and essential pathological phenomenon in dystrophic muscle fibers, which may be related to lysosomal function and muscle fiber degeneration.
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References
Asmundson, V. S., Julian, L. M.: Inherited muscle abnormality in the domestic fowl. J. Hered.47 248–252 (1956)
Banker, B. Q.: A phase and electron microscopic study of dystrophic muscle. I. The pathological changes in the two-week-old Bar Harbor 129 dystrophic mouse. J. Neuropathol. Exp. Neurol.26, 259–275 (1967)
Bird, J. W.: Skeretal muscle lysosomes. In: Lysosomes in biology and pathology, Vol. 4, pp. 75–109. Dingle, J. R., Dean, R. T. (eds). Amsterdam: North-Holland 1975
Dayton, W. R., Goll, D. E., Stromer, M. H., Reville, W. J., Zeece, M. G., Robson, R. M.: Some properties of a Ca2+-activated protease that may be involved in myofibrillar protein turnover. In: Proteases and biological control, pp. 551–577. Reich, E., Rifkin, D. B., Shaw, E. (eds.). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1975
Deams, W. Th., Wisse, L., Brederoo, P.: Electron microscopy of the vacuolar apparatus. In: Lysosomes in biology and pathology, Vol. 1, pp. 64–112. Dingle, J. T., Fell, H. B. (eds.). Amsterdam: North-Holland 1969
de Duve, C., Wattiaux, R.: Functions of lysosomes. Ann. Rev. Physiol.28, 435–492 (1966)
Engel, A. G.: Vacuolar myopathies: Multiple etiologies and sequential structural studies. In: The striated muscle, pp. 301–341, Pearson, C. M., Mostofi, F. K. (eds.). Baltimore: Williams and Wilkins 1973
Engel, A. G., MacDonald, R. D.: Ultrastructural reactions in muscle disease and their light-microscopic correlates. In: Muscle diseases: Proceedings of an internal congress, Milan, 19–20 May, 1969, pp. 99–108. Walton, J. N., Carol, N., Scarlato, G. (eds.). Amsterdam: Excerpta Medica 1969
Engel, A. G., Stonnington, H. H.: Morphological effects of denervation of muscle. A quantitative ultrastructural study. Ann. NY Acad. Sci.228, 68–88 (1974)
Gori, Z.: Proliferations of the sarcoplasmic reticulum and the T system in denervated muscle fibers. Virchows Archiv [Cell Pathol.]11, 147–160 (1972)
Ishikawa, H.: Formation of elaborate networks of T-system tubules in cultured skeletal muscle with special reference to the T-system formation. J. Cell. Biol.38, 51–66 (1968)
Jirmanová, I., Libelius, R., Lundquist, I., Thesleff, S.: Protamine induced intracellular uptake of horseradish peroxidase and vacuolation in mouse skeletal muscle in vitro. Cell Tissue Res.176, 463–473 (1977)
Julian, L. M., Asmundson, V. S.: Muscular dystrophy of the chicken. In: Muscular dystrophy in man and animals, pp. 457–498. Bourne, G. H., Golarz, M. N. (eds.). New York: Hafner 1963
Libelius, R.: Evidence for endocytotic uptake of cobra neurotoxin in mouse skeletal muscle. J. Neural. Transm.37, 61–71 (1975)
Libelius, R., Jirmanová, I., Lundquist, I., Thesleff, S.: Increased endocytosis with lysosomal activation in skeletal muscle of dystrophic mouse. J. Neuropathol. Exp. Neurol.37, 387–400 (1978a)
Libelius, R., Josefsson, J.-O., Lundquist, I.: Endocytosis in chronically denervated mouse skeletal muscle. A biochemical and ultrastructural study with horseradish peroxidase. Neuroscience4, 283–292 (1979)
Libelius, R., Lundquist, I.: Lysosomal activation in mouse skeletal muscle induced by protamine in vitro. Cell Tissue Res.186, 1–11 (1978)
Libelius, R., Lundquist, I., Templeton, W., Thesleff, S.: Intracellular uptake and degradation of extracellular tracers in mouse skeletal muscle in vitro: The effect of denervation. Neuroscience3, 641–647 (1978b)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)
Lundquist, I., Josefsson, J.-O.: Sensitive method for determination of peroxidase activity in tissue by means of coupled oxidation reaction. Anal. Biochem.41, 567–577 (1971)
Malouf, N. N., Sommer, J. R.: Chicken dystrophy. The geometry of the transverse tubules. Am. J. Pathol.84, 299–316 (1976)
Pearse, G. W.: Electron microscopy in the study of muscular dystrophy. Am. NY Acad. Sci.138, 138–150 (1966)
Pellegrino, C., Franzini, C.: An electron microscopic study of denervation atrophy in red and white skeletal muscle fibers. J. Cell Biol.17, 327–349 (1963)
Schiaffino, S., Hanzliková, V.: Studies on the effect of denervation in developing muscle. II. The lysosomal system. J. Ultrastruct. Res.39, 1–14 (1972)
Schotland, D. L.: An electron microscopic investigation of myotomic dystrophy. J. Neuropathol. Exp. Neurol.29, 241–253 (1970)
Seiden, D.: Effects of colchicine on myofilament arrangement and the lysosomal system in skeletal muscle. Z. Zellforsch144, 467–473 (1973)
Shafiq, S. A., Asiedu, S., Ryan, D., Milhorat, A. T.: Effect of denervation and hereditary muscular dystrophy on the differentiation of chicken fiber types with some observations on early changes in muscular dystrophy. In: Exploratory concepts in muscular dystrophy and related neuromuscular diseases, pp. 230–242, Milhorat, A. T. (ed.). Amsterdam: Excerpta Medica 1974
Weinstock, I. M., Iodice, A. A.: Acid hydrolase activity in muscular dystrophy and denervation atrophy. In: Lysosomes in biology and pathology, Vol. 1. pp. 450–468. Dingle, J. T., Fell, H. B. (eds.). Amsterdam: North-Holland 1969
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Libelius, R., Jirmanova, I., Lundquist, I. et al. T-Tubule endocytosis in dystrophic chicken muscle and its relation to muscle fiber degeneration. Acta Neuropathol 48, 31–38 (1979). https://doi.org/10.1007/BF00691788
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DOI: https://doi.org/10.1007/BF00691788