Summary
In this study the presence of intraepithelial cells within the normal breast parenchyma was investigated by electron microscopy and immunocytochemistry. Cells were observed which could be differentiated from the epithelial and myoepithelial cells by their cytoplasmic and nuclear morphology and the absence of cell junctions. Two cell types (lymphocytes and macrophages) were identified ultrastructurally and the bone marrow origin of the cells was confirmed by immunocytochemistry. The intraepithelial lymphocytes and macrophages were present in all samples irrespective of the physiological state. In the “resting”, pregnant, and lactating breast the majority of cells were lymphocytes while in the involuting breast there was a marked increase in the proportion of macrophages. The rarity of lymphoma of the breast may be related to the relatively small amount of lymphoid tissue present and the passive nature of the environment.
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References
Ahmed A. Atlas of the ultrastructure of human breast disease. Churchill Livingstone, 1978
Anscombe AM, Wright DH (1985) Primary malignant lymphoma of the thyroid - a tumor of the mucosa-associated lymphoid tissue: review of seventy-six cases. Histopathology 9:81–97
Bienenstock J, Befus AD (1980) Mucosal immunology. Immunology 41:249–270
Bienenstock J, McDermott M, Befus AD (1979) A common mucosal immune system. In: Ogra PL, Dayton D (eds) Immunology of breast milk, Raven Press, New York, p 91
Cordell J, Richardson TC, Pulford KAF, Ghosh AK, Gatter KC, Heyderman E, Mason DY (1985) Production of two monoclonal antibodies against human epithelial membrane antigen and their use in diagnostic immunocytochemistry
DeCosse JJ, Berg JW, Fracchia AA, Farrow JH (1962) Primary lymphosarcoma of the breast: A review of 14 cases. Cancer 15:1264–1268
Fanger H, Ree HJ (1974) Cyclic changes of human mammary gland epithelium in relation to the menstrual cycle - an ultrastructural study. Cancer 34:574–585
Ferguson DJP, Anderson TJ (1981a) Ultrastructural observations on cell death by apoptosis in the “resting” human breast. Virchows Arch [Pathol Anat] 393:193–203
Ferguson DJP, Anderson TJ (1981b) A technique for identifying areas of interest in human breast tissue before embedding for electron microscopy. J Clin Pathol 34:1187–1189
Ferguson DJP, Anderson TJ (1983a) A morphological study of the changes with occur during pregnancy in the human breast. Virchows Arch [Pathol Anat] 401:163–175
Ferguson DJP, Anderson TJ (1983b) An ultrastructural study of lactation in the human breast. Anat Embryol 168:349–359
Ghadially FN (1982) Ultrastructural pathology of the cell and matrix (2nd ed). Butterworth, London and Boston, pp 704–708
Goldblum RM, Ahlstedt S, Carlsson B, Hanson LA, Jodal U, Lidin-Janson G, Sonl-Akerlund A (1975) Antibody forming cells in human colostrum after oral immunisation. Nature 257:797–799
Helminen HJ, Ericsson JLE (1968a) Studies on mammary gland involution 1. On the ultrastructure of the lactating mammary gland. J Ultrastruct Res 25:193–213
Helminen HJ, Ericsson JLE (1968b) Studies on mammary gland involution 2. Ultrastructural evidence for auto- and heterophagocytosis. J Ultrastruct Res 25:214–227
Helminen HJ, Ericsson JLE (1972) Ultrastructural studies on prostatic involution in the rat. Evidence for focal irreversible damage to epithelium and heterophagic digestion in macrophages. J Ultrastruct Res 39:443–455
Herbert A, Wright DJ (1984) Isaacson, P. and Smith, J.L. Primary malignant lymphoma of the lung: histopathologic and immunologic evaluation of nine cases. Hum Pathol 15:415–422
Hopwood D, Levison DA (1976) Atrophy and apoptosis in the cyclical human endometrium. J Pathol 119:159–166
Isaacson P, Wright DJ (1983) Malignant lymphoma of mucosa-associated lymphoid tissue. Cancer 52:1410–1416
Isaacson P, Wright DH (1984) Extranodal malignant lymphoma arising from mucosa-associated lymphoid tissue. Cancer 53:2515–2524
Mambo NY, Burke JS, Butler JJ (1977) Primary malignant lymphomas of the breast. Cancer 39:2033–2040
Moore I, Wright DH (1984) Primary gastric lymphoma - a tumor of mucosa-associated lymphoid tissue. A histological and immunohistochemical study of 36 cases. Histopathology 8:1025–1039
Ozzello L (1971) Ultrastructure of the human mammary gland. Pathol Annual 6:1–59
Salazar H, Tobon H (1974) Morphologic changes of the mammary gland during development, pregnancy and lactation. In: Josimovich JB (ed). Lactogenic hormones, fetal nutrition and lactation. John Wiley and Sons, New York, pp 221–277
Seelig LL, Beer AE (1981) Intraepithelial leukocytes in the human mammary gland. Biol Reprod 24:1157–1163
Smith CA, Monaghan P, Neville AM (1984) Basal clear cells of the normal human breast. Virchows Arch [Pathol Anat] 402:319–329
Stirling JW, Chandler JA (1976) The fine structure of the normal, “resting” terminal ductal lobular unit of the female breast. Virchows Arch [Pathol Anat] 372:205–226
Stirling JW, Chandler JA (1977) The fine structure of ducts and subalveolar ducts in the resting gland of the female breast. Virchows Arch [Pathol Anat] 373:119–132
Strum JM, Phelps PC, McAtee MM (1983) Resting human female breast tissue produces iodinated proteins. J Ultrastruct Res 84:130–139
Svennerholm A-M, Holmgren J, Hanson LA, Lindblad BS, Quereshi F, Rahimtoola RJ (1977) Boosting of secretory IgA antibody responses in man by parenteral cholera vaccination. Scand J Immunol 6:1345–1349
Tannenbaum M, Weiss M, Marx AJ (1969) Ultrastructure of the human mammary ductule. Cancer 23:958–978
Toker C (1967) Observations on the ultrastructure of a mammary ductule. J Ultrastruct Res 21:9–25
Toner PG, Ferguson A (1971) Intraepithelial cells in the human intestinal mucosa. J Ultrastruct Res 34:329–344
Warnke RA, Gatter KC, Falini B, Hildreth P, Woolston RE, Pulford K, Cordell JL, Cohen B, de Wolf-Peters C, Mason DY (1983) Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 309:1275–1281
Waugh D, van Der Hoeven E (1962) Fine structure of the human adult female breast. Lab Invest 11:220–228
Wiseman C, Lias KT (1972) Primary lymphoma of the breast. Cancer 29:1705–1712
Wooding FBP (1977) Comparative mammary fine structure. Sym Zool Soc Lond 41:1–41
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Ferguson, D.J.P. Intraepithelial lymphocytes and macrophages in the normal breast. Vichows Archiv A Pathol Anat 407, 369–378 (1985). https://doi.org/10.1007/BF00709984
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DOI: https://doi.org/10.1007/BF00709984