Summary
The expression of intermediate filament proteins in human brain ependyma and choroid plexus epithelium has been studied by immunohistochemistry using a panel of monoclonal antibodies directed against all five classes of intermediate filaments. Ependymal cells express GFAP and vimentin filaments, whereas plexus epithelium simultaneously contains neurofilaments, cytokeratins and vimentin, a phenomenon not previously observed in normal cells in vivo. By means of specific antibodies we were able to establish that cytokeratins 8 and 18 but not 19 are present in plexus epithelium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brulet P, Babinet C, Kemler R, Jacob F (1980) Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation. Proc Natl Acad Sci (USA) 77:4113–4117
Cooper D (1985) Classification of human epithelia and their neoplasms using monoclonal antibodies to Keratins: Strategies, applications, and limitations. Laboratory Investigation 52:243–256
Dellagi K, Brouet JC, Perreau J, Paulin D (1982) Human monoclonal IgM with autoantibody activity against intermediate filaments. Proc Natl Acad Sci USA 79:446–450
Fujita T, Iwanga T, Kusumoto Y, Yoshie S (1981) Neurosecretion. Molecules. Cells. Systems. Plenum Press New York, London
Gambetti P, Autilio-Gambetti L, Papasozomenos SC (1981) Bodian's silver method stains neurofilament polypeptides. Science 213:1521–1522
Gown AM, Gabbiani G (1984) Intermediate-sized (10-nm) filaments in human tumors. In: Delellis RA (ed) Advances in Immunohistochemistry. Masson Publ USA, pp 89–109
Karsten U, Widmaier R, Kunde D (1983) Monoclonal antibodies against antigens of the human mammary carcinoma cell line MCF-7. Arch Geschwulstforsch 53:529–536
Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H (1985) Monoclonal anticytokeratin antibody from a hybridoma clone generated by electrofusion. Eur J Cancer Clin Oncol 21:733–740
Kasper M, Karsten U, Stosiek P (1986) Detection of cytokeratin(s) in epithelium of human Plexus choroideus by monoclonal antibodies. Acta Histochem 78:101–103
Lane EB (1982) Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization. J Cell Biol 92:665–673
Oksche A (1984) Das Plexusepithel: Struktur und Funktion. Verh Anat Ges 78:71–83
Shaw G, Osborn M, Weber K (1981) An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brain. Eur J Cell Biol 26:68–70
Sternberger LA, Sternberger NH (1983) Monoclonal antibodies distinguish phosphorylated and nonphosphorylated forms of neurofilaments in situ. Proc Natl Acad Sci USA 80:6126–6130
Vandevelde M, Fankhauser R, Luginbühl H (1985) Immuncytochemical studies in canine neuroectodermal brain tumors. Acta Neuropathol (Berl) 66:111–116
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kasper, M., Goertchen, R., Stosiek, P. et al. Coexistence of cytokeratin, vimentin and neurofilament protein in human choroid plexus. Vichows Archiv A Pathol Anat 410, 173–177 (1987). https://doi.org/10.1007/BF00710822
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00710822