Abstract
The capacitance of suspensions of CHO and HeLa cells (0.5–3×106 cells/ml) has been measured between 0.2 and 10 MHz. As frequencies decrease, there is a continuous increase in capacitance of both the cell suspension and the spent growth medium free of cells, a phenomenon which is partially attributed to an increased polarisation of the electrodes. At a given frequency, subtraction of the capacitance of the spent medium from that of the cell suspension allows one to determine the capacitance of the cells only. The intensity of this signal varies linearly with the biomass and cell size.
At low frequencies such as those used in this study (0.25 MHz), where sensitivity is the highest, concentrations as low as 0.5×106 cells/ml can be accurately measured.
Suggestions are made how to make these measures on-line, non-invasive and in real time.
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References
Davey CL, Kell DB, Kemp RB and Meredith RWJ (1988) On the audio- and radio-frequency dielectric behaviour of anchorage-dependent, mouse L929-derived LS fibroblasts. Bioelectrochemistry Bioenergetics 20: 83–98.
Davey CL, Markx GH and Kell DB (1990) Substitution and spreadsheet methods for analysing dielectric spectra of biological systems. Eur. Biophys. J. 18: 255–265.
Degouys V, Menozzi FD, Dubois D and Miller AOA (1993) Growth of animal cells on microbeads:In situ biomass estimation by electronic sizing. In: Griffiths JB, Doyle A and Newell DG (eds), Protocols in Cell and Tissue Culture. J. Wiley Publ. (in press).
Fehrenbach R, Comberbach M and Pêtre JO (1992) On-line biomass monitoring by capacitance measurement. J. Biotechnol. 23: 303–314.
Hanotte O, Dejaiffe C, Dubois D, Vanderpoorten P, Menozzi FD and Miller AOA (1987) Growth of animal cells on microbeads. II. Estimation of biomassin situ and observation of cytopathic effects after infection of McCoy cells with Chlamydia trachomatis. Biol. Cell. 59: 175–180.
Harris CM, Todd RW, Bungard SJ, Lovitt RW, Morris JG and Kell DB (1987) The dielectric permitivity of microbial suspensions at radio frequencies: a novel method for the real-time estimation of microbial biomass. Enz. Micr. Technol. 9: 181–186.
Irimajiri A, Asami K, Ichinowatari T and Kinoshita Y (1987a) Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. I. Dielectric behavior of cell suspensions in 0.01–500 MHz and its simulation with a singl shell model. Biochim. Biophys. Acta 896: 203–213.
Irimajiri A, Asami K, Ichinowatari T and Kinoshita Y (1987b) Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. II. Effects of osmotic perturbation. Biochim. Biophys. Acta 896: 214–223.
Kell DB, Markx GH, Davey CL and Todd RW (1990) “Real-time” monitoring of cellular biomass: methods and applications. Trends Anal. Chem. 9: 190–194.
Markx GH, Davey CL, Kell DB and Morris P (1991) The dielectric permittivity at radio frequencies and the Bruggeman probe: novel techniques for the on-line determination of biomass concentrations in plant cell cultures. J. Biotechnol. 20: 279–290.
Miller AOA (1972) Study of RNA extracted from HeLa cell polysomes. Isopycnic centrifugation of cytoplasmic particles extracted from normal, poliovirus-infected HeLa cells and mouse A9 cells. Arch. Biochem. Biophys. 150: 282–295.
Miller SJO, Henrotte M and Miller AOA (1986) Growth of animal cells on microbeads. I.In situ estimation of numbers. Biotechnol. Bioeng. 28: 1466–1473.
Stoicheva NG, Davey CL, Markx GH and Kell DB (1989) Dielectric spectroscopy a rapid method for the determination of solvent biocompatibility during biotransformations. Biocatalysis 2: 245–255.
Thebline D, Harfield J, Hanotte O, Dubois D and Miller AOA (1987) Growth of animal cells on microbeads. III. Semicontinuousin situ estimation of numbers. In: Spier RE and Griffiths JB (eds), Modern Approaches to Animal Cell Technology (pp 504–512), Butterworths Publ.
Todd R (1993) Personal communication.
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Gerckel, I., Garcia, A., Degouys, V. et al. Dielectric spectroscopy of mammalian cells. Cytotechnology 13, 185–193 (1993). https://doi.org/10.1007/BF00749814
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DOI: https://doi.org/10.1007/BF00749814