Abstract
Low-frequency dielectric spectroscopy has been usedin situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture Impedance Pulse Spectroscopy (also called electronic sizing), is characterized by a somewhat lower degree of resolution. Suggestions are made on how to determine the capacitance of the spent growth medium alone, still keeping the probe inserted in the bioreactor. This will make dielectric spectroscopy the first trulyin situ, on-line, in real time, non-invasive measure of the biomass.
Similar content being viewed by others
References
Cerckel I, Garcia A, Degouys V, Dubois D, Fabry L and Miller AOA (1993) Dielectric spectroscopy of mammalian cells. 1. Evaluation of the biomass of HeLa- and CHO cells in suspension by low-frequency dielectric spectroscopy. Cytotechnology @@@ (this issue).
Davey CL, Markx GH and Kell DB (1990) Substitution and spreadsheet methods for analysing dielectric spectra of biological systems. Eur. Biophys. J. 18: 255–265.
Degouys V, Menozzi FD, Dubois D, Fabry L and Miller AOA. Evolution of the capacitance of Cytodex 3 microbeads suspended in growth medium. In: Spier RE and Griffiths JB (eds.), Proceedings of the 12th ESACT Animal Cell Technology: Products for Today, Prospects for Tomorrow. Butterworth/Heinemann Publ. (forthcoming).
Degouys V, Menozzi FD, Dubois D and Miller AOA (1994) Growth of animal cells on microbeads:In situ biomass estimation by electronic sizing. In: Doyle A, Griffiths JB and Newell DG (eds.) Protocols in Cell & Tissue Culture. J. Wiley Publ. (in print).
Fehrenbach R, Comberbach M and Pêtre JO (1992) On-line biomass monitoring by capacitance measurement. J. Biotechnol. 23: 303–314.
Ferris LE, Davey CL and Kell DB (1990) Evidence from its temperature dependence that the β-dielectric dispersion of cell suspensions is not due solely to the charging of a static membrane capacitance. Eur. Biophys. J. 18: 267–276.
Markx GH, Davey CL, Kell DB and Morris P (1991) The dielectric permittivity at radio frequencies and the Bruggeman probe: novel techniques for the on-line determination of biomass concentrations in plant cell cultures. J. Biotechnol. 20: 279–290.
Miller-Faurès A, Michel N, Aguilera A, Blave A and Miller AOA (1981) Laser flow cytofluorometric analysis of HTC cells synchronized with hydroxyurea, Nocodazole and Aphidicolin. Cell Tissue Kinet. 14: 501–514.
Miller SJO, Henrotte M and Miller AOA (1986) Growth of animal cells on microbeads. I.In situ estimation of numbers. Biotech. Bioeng. 28: 1466–1473.
Miller AOA, Menozzi FD and Dubois D (1989) Microbeads and anchorage dependent eukaryotic cells: The beginning of a new era in biotechnology. Adv. Biochem. Eng/Biotechnol 39: 73–95.
Thebline D, Harfield J, Hanotte O, Dubois D and Miller AOA (1987) Growth of animal cells on microbeads. III. Semi continuousin situ estimation of numbers. In: Spier RE and Griffiths JB (eds.), Modern Approaches to Animal Cells Technology, (pp. 504–512). Butterworth/Heinemann Publ.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Degouys, V., Cerckel, I., Garcia, A. et al. Dielectric spectroscopy of mammalian cells. Cytotechnology 13, 195–202 (1993). https://doi.org/10.1007/BF00749815
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00749815