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The potential of AFLPs in biosystematics: A first application inSolanum taxonomy(Solanaceae)

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Abstract

Using the AFLP technique highly informative DNA fingerprints were generated from 19 taxa ofSolanum sect.Petota (potatoes) and three taxa ofSolanum sect.Lycopersicum (tomatoes). Both phenetic and cladistic analyses were conducted from the individual genotypic level to the species level. An AFLP fingerprint, using a combination of suitable AFLP primers, generated 12 to 71 scorable fragments per genotype which was sufficient for taxonomic interpretation. The classifications based on the molecular markers were generally in agreement with current taxonomic opinions. Unexpectedly,S. microdontum was associated with ser.Megistacroloba rather than with ser.Tuberosa, andS. demissum (ser.Demissa) and species of ser.Acaulia appeared closely affiliated. AFLP is an efficient and reliable technique to generate biosystematic data and therefore a promising tool for evolutionary studies.

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References

  • Bachmann, K., 1992: Nuclear DNA markers in angiosperm taxonomy. — Acta Bot. Neerl.41: 369–384.

    Google Scholar 

  • —, 1995: Progress and pitfalls in systematics: cladistics, DNA and morphology. — Acta Bot. Neerl.44: 403–419.

    Google Scholar 

  • Ballvora, A., Hesselbach, J., Niewöhner, J., Leister, D., Salamini, F., Gebhardt, C., 1995: Marker enrichment and high-resolution map of the segment of potato chromosome VII harbouring the nematode resistance geneGro1. — Molec. Gen. Genet.249: 82–90.

    PubMed  Google Scholar 

  • Bamberg, J. B., Martin, M. W., 1993: Inventory of tuber-bearingSolanum species. Catalog of potato germplasm. — Potato Introduction Station, NRSP-6, Sturgeon Bay, USA.

  • Becker, J., Vos, P., Kuiper, M., Salamini, F., Heun, M., 1995: Combined mapping of AFLP and RFLP markers in barley. — Molec. Gen. Genet.249: 65–73.

    PubMed  Google Scholar 

  • Bernatzky, R., Tanksley, S. D., 1986: Genetics of actin regulated sequences in tomato. — Theor. Appl. Genet.72: 314–321.

    Google Scholar 

  • Camadro, E. L., Masuelli, R. W., Cortés, M. C., 1992: Haploids of the wild tetraploid potatoSolanum acaule ssp.acaule: generation, meiotic behavior, and electrophoretic pattern for the aspartate aminotransferase system. — Genome35: 431–453.

    Google Scholar 

  • Chase, M. W., Soltis, D. E., Olmstead, R. G., Morgan, D., Les, D. H., Mishler, B. D., Duvall, M. R., Price, R. A., Hills, H. G., Qiu, Y.-L., Kron, K. A., Rettig, J. H., Conti, E., Palmer, J. D., Manhart, J. R., Sytsma, K. J., Michaels, H. J., Kress, W. J., Karol, K. G., Clark, W. D., Hedren, M., Gaut, B. S., Jansen, R. K., Kim, K.-J., Wimpee, C. F., Smith, J. F., Furnier, G. R., Straus, S. H., Xiang, Q., Plunkett, G. M., Soltis, P. S., Swenson, S. M., Williams, S. E., Gadek, P. A., Quinn, C. J., Eguiarte, L. E., Golenberg, E., Learn, G. H., Graham, S. W., Barrat, S. C. H., Dayanandan, S., Albert, V. A., 1993: Phylogenetics of seed plants: an analysis of nucleotide sequences from the plastid generbcL. — Ann. Missouri Bot. Gard.80: 528–580.

    Google Scholar 

  • Child, A., 1990: A synopsis ofSolanum subgenusPotatoe (G. Don)D'Arcy (Tuberarium (Dunal)Bitter (s. l.)). — Feddes Repert.101: 209–235.

    Google Scholar 

  • Correll, D. S., 1962: The potato and its wild relatives. — Renner: Texas Research Foundation.

    Google Scholar 

  • Debener, T., Salamini, F., Gebhardt, C., 1990: Phylogeny of wild and cultivatedSolanum species based on nuclear restriction fragment length polymorphisms (RFLPs). — Theor. Appl. Genet.79: 360–368.

    Google Scholar 

  • Dice, L. R., 1945: Measures of the amount of ecologic association between species. — Ecology26: 297–302.

    Google Scholar 

  • Farris, J. S., 1970: Methods for computing Wagner trees. — Syst. Zool.19: 83–92.

    Google Scholar 

  • Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using bootstrap. — Evolution39: 783–791.

    Google Scholar 

  • Folkertsma, R. T., Rouppe van der Voort, J. N. A. M., De Groot, K. E., Van Zandvoort, P. M., Schots, A., Gommers, F. J., Helder, J., Bakker, J., 1996: Gene pool similarities of potato cyst nematode populations assessed by AFLP analysis. — Molec. Pl.-Microbe Interact.9: 47–54.

    Google Scholar 

  • Giannattasio, R. B., Spooner, D. M., 1994: A reexamination of species boundaries betweenSolanum megistacrolobum andSolanum toralapanum (Solanum sect.Petota, ser.Megistacroloba): morphological data. — Syst. Bot.19: 89–105.

    Google Scholar 

  • Hawkes, J. G., 1962: The origin ofSolanum juzepczukii Buk. andSolanum curtilobum Juz. etBuk. — Z. Pflanzenzücht.47: 1–14.

    Google Scholar 

  • —, 1990: The potato: evolution, biodiversity and genetic resources. — London: Belhaven Press.

    Google Scholar 

  • Hosaka, K., Ogihara, Y., Matsubayashi, M., Tsunewaki, K., 1984: Phylogenetic relationship between the tuberousSolanum species as revealed by restriction endonuclease analysis of chloroplast DNA. — Jap. J. Genet.59: 349–369.

    Google Scholar 

  • Huys, G., Coopman, R., Janssen, P., Kersters, K., 1996: High-resolution genotypic analysis of the genusAeromonas by AFLP fingerprinting. — Int. J. Syst. Bacteriol.46: 572–580.

    PubMed  Google Scholar 

  • Janssen, P., Coopman, R., Huys, G., Swings, J., Bleeker, M., Vos, P., Zabeau, M., Kersters, K., 1996: Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. — Microbiology142: 1881–1893.

    PubMed  Google Scholar 

  • Kardolus, J. P., 1997: Morphological variation within ser.Acaulia (Solanum sect.Petota).— InNee, M., Simon D., Jessop, J. P., (Eds):Solanaceae IV: progress in biology and utilisation. — Richmond: Royal Botanical Gardens, Kew, (in press).

    Google Scholar 

  • Lindhout, P., Pet, G., Van der Beek, H., 1994: Screening wildLycopersicon species for resistance to powdery mildew (Oidium lycopersicum). — Euphytica72: 43–49.

    Google Scholar 

  • Marks, G. E., 1955: Cytogenetic studies in tuberousSolanum species. I. Genomic differentiation in the groupDemissa. — J. Genet.53: 262–269.

    Google Scholar 

  • —, 1965: Cytogenetic studies in tuberousSolanum species. III. Species relationships in some South and Central American species. — New Phytol.64: 293–306.

    Google Scholar 

  • Matsubayashi, M., 1991: Phylogenetic relationships in the potato and its related species. — InTsuchiya, T., Gupta, P. K., (Eds): Chromosome engineering in plants: genetics, breeding, evolution. Part B, pp. 93–118. — Amsterdam: Elsevier.

    Google Scholar 

  • McDade, L., 1990: Hybrids and phylogenetic systematics. I. Patterns of character expression in hybrids and their implications for cladistic analysis. — Evolution44: 1685–1700.

    Google Scholar 

  • Meksem, K., Leister, D., Peleman, J., Zabeau, M., Salamini, F., Gebhardt, C., 1995: A high-resolution map of the vicinity of theR1 locus on chromosome V of potato based on RFLP and AFLP markers. — Molec. Gen. Genet.249: 74–81.

    PubMed  Google Scholar 

  • Miller, J. T., Van den Berg, R. G., Ugarte, M. L., Kardolus, J. P., Villand, J., Spooner, D. M., 1996: Morphological and molecular systematics of the wild potatoSolanum brevicaule complex — Amer. J. Bot.83: 179 (abstract).

    Google Scholar 

  • Mueller, U. G., Lipari, S. E., Milgroom, M. G., 1996: Amplified fragment length polymorphism (AFLP) fingerprinting of symbiotic fungi cultured by the fungusgrowing antCyphomyrmex minutus. — Molec. Ecol.5: 119–122.

    Google Scholar 

  • Olmstead, R. G., Palmer, J. D., 1994: Chloroplast DNA systematics: a review of methods and data analysis. — Amer. J. Bot.81: 1205–1224.

    Google Scholar 

  • Rohlf, F. J., 1993: NTSYS-PC. Numerical taxonomy and multivariate analysis system. Version 1.80. — New York: Applied Biostatistics Inc.

    Google Scholar 

  • Rouppe van der Voort, J. N. A. M., Van Zandvoort, P. M., Van Eck, H. J., Folkertsma, R. T., Hutten, R. C. B., Draaistra, J., Gommers, F. J., Jacobsen, E., Helder, J., Bakker, J., 1997: Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. — Molec. Gen. Genet.255: 438–447.

    PubMed  Google Scholar 

  • Schmiediche, P. E., Hawkes, J. G., Ochoa, C. M., 1982: The breeding of the cultivated potato speciesS. ×juzepczukii andS. ×curtilobum. II. The resynthesis ofS. ×juzepczukii andS. ×curtilobum. — Euphytica31: 695–707.

    Google Scholar 

  • Smith, J. J., Scott-craig, J. S., Leadbetter, J. R., Bush, G. L., Roberts, D. L., Fulbright, D. W., 1994: Characterization of random amplified polymorphic DNA (RAPD) products fromXanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis. — Molec. Phylogenet. Evol.3: 135–145.

    PubMed  Google Scholar 

  • Soltis, P. S., Soltis, D. E., Doyle, J. J., 1992: Molecular systematics of plants. — New York, London: Chapman & Hall.

    Google Scholar 

  • Spooner, D. M., Sytsma, K. J., Conti, E., 1991: Chloroplast DNA evidence for genome differentiation in wild potatoes (Solanum sect.Petota:Solanaceae). — Amer. J. Bot.78: 1354–1366.

    Google Scholar 

  • —, 1993: Chloroplast DNA evidence for the interrelationships of tomatoes, potatoes, and pepinos (Solanaceae). — Amer. J. Bot.80: 676–688.

    Google Scholar 

  • —, 1995: Examination of species boundaries ofSolanum seriesDemissa and potentially related species in ser.Acaulia and ser.Tuberosa (sect.Petota). — Syst. Bot.20: 295–314.

    Google Scholar 

  • —, 1996: Comparison of four molecular markers in measuring relationships among the wild potato relativesSolanum sectionEtuberosum (subgenusPotatoe). — Theor. Appl. Genet.92: 532–540.

    Google Scholar 

  • Swofford, D. L., 1993: PAUP: phylogenetic analysis using parsimony, version 3.1. Computer program. — Champaign: Illinois Natural History Survey.

    Google Scholar 

  • Ugent, D., 1966: Hybrid weed complexes inSolanum, sectionTuberarium. — Ph.D. Dissertation, University of Wisconsin, USA.

    Google Scholar 

  • Van den Berg, R. G., Groendijk-Wilders, N., 1997: Numerical analysis of the taxa ofCircaeifolia (Solanum sect.Petota). — InNee, M., Simon, D., Jessop, J. P., (Eds):Solanaceae IV: progress in biology and utilisation. — Richmond: Royal Botanical Gardens, Kew, (in press).

    Google Scholar 

  • —, 1996: The wild ancestors of cultivated potato: the brevicaulecomplex. — Acta Bot. Neerl.45: 157–171.

    Google Scholar 

  • Van Eck, H. J., Rouppe van der Voort, J., Draaistra, J., Van Zandvoort, P., Van Enckevort, E., Segers, B., Peleman, J., Jacobsen, E., Helder, J., Bakker, J., 1995: The inheritance and chromosomal localization of AFLP markers in a non-inbred potato offspring. — Molec. Breeding1: 397–410.

    Google Scholar 

  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M., 1995: AFLP: a new technique for DNA fingerprinting. — Nucl. Acids Res.23: 4407–4414.

    PubMed  Google Scholar 

  • Whitkus, R., Doebley, J., Wendel, J. F., 1994: Nuclear DNA markers in systematics and evolution. — InPhillips, R. L., Vasil, I. K., (Eds): DNA-based markers in plants, pp. 118–141. — Dordrecht: Kluwer.

    Google Scholar 

  • Williams, C. E., St. Clair, D. A., 1993: Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions ofLycopersicon esculentum. — Genome36: 619–630.

    Google Scholar 

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Kardolus, J.P., van Eck, H.J. & van den Berg, R.G. The potential of AFLPs in biosystematics: A first application inSolanum taxonomy(Solanaceae) . Pl Syst Evol 210, 87–103 (1998). https://doi.org/10.1007/BF00984729

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  • DOI: https://doi.org/10.1007/BF00984729

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