Abstract
The root hairs of plants are tubular projections of root epidermal cells and are suitable for investigating the control of cellular morphogenesis. In wild-typeArabidopsis thaliana (L.) Heynh, growing root hairs were found to exhibit cellular expansion limited to the apical end of the cell, a polarized distribution of organelles in the cytoplasm, and vesicles of several types located near the growing tip. Therhd3 mutant produces short and wavy root hairs with an average volume less than one-third of the wild-type hairs, indicating abnormal cell expansion. The mutant hairs display a striking reduction in vacuole size and a corresponding increase in the relative proportion of cytoplasm throughout hair development. Bead-labeling experiments and ultrastructural analyses indicate that the wavy-hair phenotype of the mutant is caused by asymmetric tip growth, possibly due to abnormally distributed vesicles in cortical areas flanking the hair tips. It is suggested that a major effect of therhd3 mutation is to inhibit vacuole enlargement which normally accompanies root hair cell expansion.
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References
Bartnicki-Garcia S (1990) Role of vesicles in apical growth and a new mathematical model of hyphal morphogenesis. In: Heath IB (ed) Tip growth in plant and fungal cells. Academic Press, San Diego, pp 211–232
Bartnicki-Garcia S, Bartnicki DD, Gierz G (1995) Determinants of fungal cell wall morphology: the vesicle supply center. Can J Bot (Suppl. 1): S372
Bates TR, Lynch JP (1996) Stimulation of root hair elongation inArabidopsis thaliana by low phosphorus availability. Plant Cell Environ, in press
Boller T, Wiemken A (1986) Dynamics of vacuolar compartmentation. Annu Rev Plant Physiol 37: 137–164
Cosgrove D (1986) Biophysical control of plant cell growth. Annu Rev Plant Physiol 37: 377–405
Cosgrove D (1993) Water uptake by growing cells: an assessment of the controlling roles of wall relaxation, solute uptake, and hydraulic conductance. Int J Plant Sci 154: 10–21
Derksen J, Emons AM (1990) Microtubules in tip growth systems. In: Heath IB (ed) Tip growth in plant and fungal cells. Academic Press, San Diego, pp 147–181
Derksen J, Rutten T, Van Amstel T, De Win A, Doris F, Steer M (1995) Regulation of pollen tube growth. Acta Bot Neerl 44: 93–119
Dolan L, Duckett CM, Grierson C, Linstead P, Schneider K, Lawson E, Dean C, Poethig S, Roberts K (1994) Clonal relationships and cell patterning in the root epidermis ofArabidopsis. Development 120: 2465–2474
Emons AM (1987) The cytoskeleton and secretory vesicles in root hairs ofEquisetum andLimnobium and cytoplasmic streaming in root hairs ofEquisetum. Ann Bot 60: 625–632
Emons AMC, Traas JA (1986) Coated pits and coated vesicles on the plasma membrane of plant cells. Eur J Cell Biol 41: 57–64
Galway ME, Heckman JW Jr, Hyde GJ, Fowke LC (1995) Advances in high-pressure and plunge-freeze fixation. In: Galbraith DW, Bourque DP, Bohnert HJ (eds) Methods in cell biology. Academic Press, San Diego, pp 3–19
Heath, IB (1985) Evidence against a direct role for cortical actin arrays in saltatory organelle motility in hyphae of the fungusSaprolegnia ferax. J Cell Sci 91: 41–47
Kiss JZ, Giddings TH Jr, Staehelin LA, Sack FD (1990) Comparison of the ultrastructure of conventionally fixed and high pressure frozen/freeze substituted root tips ofNicotiana andArabidopsis. Protoplasma 157: 64–74
Kushida H (1974) A new method for embedding with a low viscosity epoxy resin “Quetol 651”. J Electron Microsc (Jpn) 23: 197
Ridge RW (1988) Freeze-substitution improves the ultrastructural preservation of legume root hairs. Bot Mag Tokyo 101: 427–441
Ridge RW (1995) Micro-vesicles, pyriform vesicles and macrovesicles associated with the plasma membrane in the root hairs ofVicia hirsuta after freeze-substitution. J Plant Res 108: 363–368
Ridge RW (1996) Root hairs: cell biology and development. In: Y Waisel, A Eshel, U Kafkafi (eds) Plant roots. The hidden half, 2nd edn. Marcel Dekker, New York, pp 127–147
Schiefelbein JW, Shipley A, Rowse P (1992) Calcium influx at the tip of growing root-hair cells ofArabidopsis thaliana. Planta 187: 455–459
Schiefelbein JW, Somerville C (1990) Genetic control of root hair development inArabidopsis thaliana. Plant Cell 2: 235–243
Schnepf E (1986) Cellular polarity. Annu Rev Plant Physiol 37: 23–47
Sherrier DJ, VandenBosch KA (1994) Secretion of cell wall polysaccarides inVicia root hairs. Plant J 5: 185–195
Sievers A, Schnepf E (1981) Morphogenesis and polarity of tubular cells with tip growth. In: Kiermayer O (ed) Cytomorphogenesis in plants. Springer, Wien, pp 265–299
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26: 31–43
Staebell M, Soll DR (1985) Temporal and spatial differences in cell wall expansion during bud and mycelium formation inCandida albicans. J Gen Microbiol 131: 1467–1480
Taiz L (1984) Plant cell expansion: regulation of cell wall mechanical properties. Annu Rev Plant Physiol 35: 585–657
Wiebe HH (1978) The significance of plant vacuoles. BioScience 28: 327–331
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Galway, M.E., Heckman, J.W. & Schiefelbein, J.W. Growth and ultrastructure ofArabidopsis root hairs: therhd3 mutation alters vacuole enlargement and tip growth. Planta 201, 209–218 (1997). https://doi.org/10.1007/BF01007706
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DOI: https://doi.org/10.1007/BF01007706