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Mutagenesis inClostridium acetobutylicum

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Summary

Methanesulfonic acid ethyl ester (EMS) is effective in inducing mutants resistant to ampicillin, erythromycin, and butanol (15 g/l). Optimal mutagenesis occurs at 85–90% kill corresponding to a 15 minute exposure to 1.0% (v/v) EMS at 35 C. At optimal conditions, the frequency of resistant mutant CFU/ total CFU plated increases 100–200 fold.

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Authors and Affiliations

  1. Moffett Technical Center, CPC International, P.O. Box 345, 60501, Argo, Illinois, USA

    S. A. Lemmel

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  1. S. A. Lemmel
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Lemmel, S.A. Mutagenesis inClostridium acetobutylicum . Biotechnol Lett 7, 711–716 (1985). https://doi.org/10.1007/BF01032281

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  • DOI: https://doi.org/10.1007/BF01032281

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