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Alteration of human follicular fluid plasminogen activator activity by ovarian hyperstimulation

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Abstract

Recently, it has been shown that granulosa-cell secretion of plasminogen activator (PA) is responsive to luteinizing hormone (LH)/human chorionic gonadotropin (hCG) as well as follicle stimulating hormone (FSH) in the rat and the pig. Accordingly, we asked whether PA activity in follicular fluid from exogenously stimulated human follicles was different from that of normal cycles and whether or not these activities correlated with follicular maturation as determined by follicular fluid steroid concentration. Follicular aspirates were obtained from women who were participating in an in vitro fertilization protocol. Follicular fluid concentrations of estradiol and progesterone were determined by established radioimmunoassay. PA activity, determined using a modified indirect solid-phase radioassay, was significantly less in follicles from patients treated with human menopausal gonadotropin (hMG) plus clomiphene (P<0.05) compared to untreated patients or those receiving hMG or clomiphene alone. Correlations of PA activity and follicular fluid steroid concentrations demonstrated no significant correlation in samples from treated patients. In contrast, untreated spontaneously cycling patients had a significant (r=0.89,P<0.05), positive correlation between follicular fluid estradiol levels. There was no correlation between PA activity and follicular fluid progesterone levels in any of the groups. These results suggest that a subtle balance in granulosa-cell secretion of PA and steroids exists, which appears to be disrupted by follicular hyperstimulation during treatment of patients participating in in vitro fertilization protocols.

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References

  1. Granelli-Piperno A, Reich E: A study of proteases and protease-inhibitor complexes in biological fluids. J Exp Med 1978;148:223–234

    PubMed  Google Scholar 

  2. Strickland S, Beers W: Studies on the role of plasminogen activator in ovulation. J Biol Chem 1976;251:5694–5702

    PubMed  Google Scholar 

  3. Beers W, Strickland S: A cell culture assay for follicle-stimulating hormone. J Biol Chem 1978;253:3877–3881

    PubMed  Google Scholar 

  4. Liu WK, Burleigh BD, Ward D: Steroid and plasminogen activator production by cultured rat granulosa cells in response to hormone treatment. Mol Cell Endocrinol 1981;21:63–73

    PubMed  Google Scholar 

  5. Beers W: Follicular plasminogen and plasminogen activator and the effect of plasmin on ovarian follicle wall. Cell 1975;6:379–386

    PubMed  Google Scholar 

  6. Beers W, Strickland S, Reich E: Ovarian plasminogen activator: Relationship to ovulation and hormonal regulation. Cell 1975;6:387–394

    PubMed  Google Scholar 

  7. Wang C: Gonadotropins regulate plasminogen activator production by rat granulosa cells. Endocrinology 1983; 112:1201–1207

    PubMed  Google Scholar 

  8. Too SKL, Bryant-Greenwood GD: The role of relaxin and the differential production, in vitro, of plasminogen activator by periovulatory rat granulosa cells. 65th Annual Meeting of the Endocrine Society, Abstr No. 856, 1983

  9. Shaw KJ, Campeau JC, Roche PC, diZerega GS: Porcine granulosa cell production of plasminogen activator: Disparity between the effects of hCG and pFSH. Endokrinologie (in press), 1985

  10. Marrs RP, Vargyas JM, Saito H, Gibbons WE, Berger T, Mishell DR, Jr: Clinical application of techniques used in human in vitro fertilization research. Am J Obstet Gynecol 1983;477–483

  11. Mishell DR, Nakamura RM, Stone SG, Kharma K, Nagata Y, Thorneycroft IH: Serum gonadtoropin and steroid paterns during the normal menstrual cycle. Am J Obstet Gynecol 1971;111:60–65

    PubMed  Google Scholar 

  12. Roche PC, Campeau JD, Shaw ST Jr: Comparative electrophoretic analysis of human and porcine plasminogen activators in SDS-polyacrylamide gels containing plasminogen and casein. Biochim Biophys Acta 1983;745:82–89

    PubMed  Google Scholar 

  13. Roche PC, Campeau JD, Shaw ST Jr: A rapid and highly sensitive solid-phase radioassay for plasminogen activators. Thromb Res (in press), 1984

  14. Wijnegaards G, Kluft C, Groenveld E: Demonstration of urokinase-related fibrinolytic activity in human serum. Br J Haematol 1982;51:165–169

    PubMed  Google Scholar 

  15. Croyton FE, Cowdon DJ: Applied General Statistics, 2nd ed. Englewood Cliffs, Prentice Hall, 1965, p 722

    Google Scholar 

  16. Channing CP, Anderson LD, Hoover DJ, Kolena J, Osteen KG, Pomgrantz SH, Tanabe K: The role of non steroidal regulators in control of oocyte and follicular maturation. Res Prog Horm Res 1982;38:331–408

    Google Scholar 

  17. diZerega GS, Campeau JD, Nakamura RM, Ujita EL, Lobo R, Marrs RP: Activity of a human follicular fluid protein(s) in spontaneous and induced ovarian cycles. J Clin Endocrinol Metab 1983;57:838–846

    PubMed  Google Scholar 

  18. diZerega GS, Marrs RP, Lobo R, Ujita EL, Brown J, Campeau JD: Correlation of inhibin and follicle regulatory protein activities with follicular fluid steroid levels in anovulatory patients. Fertil Steril 1984;41:849–855

    PubMed  Google Scholar 

  19. Fowler RE, Edwards RG, Walters DE, Chan STH, Steptoe PC: Steroidogenesis in preovulatory follicles of patients given human meopausal and chorionic gonadotropins as judged by radioimmunoassay of steroids in follicular fluids J Endocrinol 1978;77:161–168

    PubMed  Google Scholar 

  20. Channing CP, Liv CQ, Jones GS, Jones H: Decline of feilicular oocyte maturation inhibitor coincident with maturation and achieveentk of fertilizability of oocytes recovered at midcycle of gonadotropin-treated women. Proc Natl Acad Sci USA 1983;80:4184–4188

    PubMed  Google Scholar 

  21. Marrs RP, Vargyas JM, Lobo R: Comparison of the intrafollicular hormonal mileau with fertilization of human oocytes in vitro (submitted for publication)

  22. Marrs RP, Lobo R, Campeau JD, Nakamura RM, Brown H, Ujita EL, diZerega GS: Correlation of human follicular fluid inhibin activity with spontaneous and induced follicle maturation. J Clin Endocrinol Metab 1984;58:704–709

    PubMed  Google Scholar 

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Supported in part by Clinical Investigator Award HD-00401 and by the University of Southern California Faculty Research and Innovation Award.

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Weimer, S.L., Campeau, J.D., Marrs, R.P. et al. Alteration of human follicular fluid plasminogen activator activity by ovarian hyperstimulation. J Assist Reprod Genet 1, 263–266 (1984). https://doi.org/10.1007/BF01131626

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