Abstract
An RFLP linkage map of peanut has been developed for use in genetic studies and breeding programs aimed at improving the cultivated species (Arachis hypogaea L.). An F2 population derived from the interspecific hybridization of two related diploid species in the sectionArachis (A. stenosperma ×A. cardenasii) was used to construct the map. Both random genomic and cDNA clones were used to develop the framework of the map. In addition, three cDNA clones representing genes coding for enzymes involved in the lipid biosynthesis pathway have been mapped in peanut. Of the 100 genomic and 300 cDNA clones evaluated, 15 and 190, respectively, revealed polymorphisms among the parents of our mapping population. Unfortunately, a large number of these produced complex banding patterns that could not be mapped. Of the 132 markers analyzed for segregation, 117 are distributed among 11 linkage groups, while 15 have not yet been associated with any other marker. A total map distance of approximately 1063 cM has been covered to-date.
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References
Amaya F, Young CT, Hammons RO, Martin G (1977) The tryptophan content of the U.S. commercial and some South American wild genotypes of the genusArachis: A survey. Oleagineux 32:225–229
Apuya N, Frazier BL, Keim P, Roth EJ, Lark KG (1988) Restriction length polymorphisms as genetic markers in soybean,Glycine max (L.) Merr. Theor Appl Genet 75:889–901
Beckmann JS, Soller M (1986) Restriction fragment length polymorphisms and genetic improvement of agricultural species. Euphtica 35:111–124
Cherry JP (1977) Potential sources of peanut seed proteins and oil in the genusArachis. J Agric Food Chem 25:186–193
Gregory WC, Gregory MP, Krapovickas A, Smith BW, Yarbrough JA (1973) Structure and genetic resources of peanuts. In: Peanuts — culture and uses. Am Peanut Res Educ Assoc, Stillwater, Oklahoma, pp 47–133
Grieshammer U, Wynne JC (1990) Mendelian and non-Mendelian inheritance of three isozymes in peanut (Arachis hypogaea L.). Peanut Sci 17:101–105
Halward TM, Stalker HT, LaRue EA, Kochert G (1991) Genetic variation detectable with molecular markers among unadapted germplasm resources of cultivated peanut and related wild species. Genome 34:1013–1020
Halward TM, Stalker HT, LaRue EA, Kochert G (1992) Use of single-primer DNA amplifications in genetic studies of peanut. Plant Mol Biol 18:315–325
Hong JC, Nagao RT, Key JL (1987) Characterization and sequence analysis of a developmentally regulated putative cell wall protein gene isolated from soybean. J Biol Chem 17:8367–8376
ICRISAT (1982) Annual Report. Patancheru, A.P., India
Keim P, Dier BW, Olson TC, Shoemaker RC (1990) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735–742
Kochert G, Halward TM, Branch WD, Simpson CE (1991) RFLP variability in peanut cultivars and wild species. Theor Appl Genet 81:565–570
Krapovickas A (1969) Evolution of the genusArachis. Seminario Advanzado de Genetica Agricola para America Latina, Maracay, Venezuela SAGA/B(d): 1–4
McCouch SR, Kochert G, Yu ZG, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Miller JC, Tanksley SD (1990) RFLP analysis of phylogenetic relationships and genetic variation in the genusLycopersicon. Theor Appl Genet 80:437–448
Moss JP (1980) Wild species in the improvement of groundnuts. In: Summerfield RJ, Bunting AH (eds) Advances in legume science vol. 1. Royal Botanic Gardens, Kew
Resslar PM, Gregory WC (1979) A cytological study of three diploid species of the genusArachis. J Hered 70:13–16
Rigby P, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J Mol Biol 113:237–251
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Simpson CE (1991) Global collaborations find and conserve the irreplaceable genetic resources of wild peanut in South America. Diversity 7:59–61
Singh AK (1986a) Utilization of wild relatives in the genetic improvement ofArachis hypogaea L. 7. Autotetraploid production and prospects in interspecific breeding. Theor Appl Genet 72:164–169
Singh AK (1986b) Utilization of wild relatives in the genetic improvement ofArachis hypogaea L. 8. Synthetic amphidiploids and their importance in interspecific breeding. Theor Appl Genet 72:433–439
Singh AK, Moss JP (1984) Utilization of wild relatives in genetic improvement ofArachis hypogaea L. 5. Genome analysis in sectionArachis and its implication in gene transfer. Theor Appl Genet 68:355–364
Southern EM (1975) Detection of specific enzyme sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Stalker HT (1991) A morphological appraisal of wild species in sectionArachis of peanuts. Peanut Sci 17:117–122
Stalker HT (1992) UtilizingArachis germplasm resources. In: Proc 2nd Int. Wkshp on Groundnut, ICRISAT, Patancheru, A.P., India, pp 24–29 (in press)
Stalker HT, Jones TM, Murphy JP (1990) Isozyme variability amongArachis species. Proc Am Peanut Res Educ Soc 22:50
Stalker HT, Wynne JC (1979) Cytology of interspecific hybrids in sectionArachis of peanuts. Peanut Sci 6:110–114
Subrahmanyam P, McDonald D, Gibbons RW, Nigam SN, Nevill DJ (1982) Resistance to rust and late leafspot diseases in some genotypes ofArachis hypogaea. Peanut Sci 9:9–14
Wilimzig R (1985) LiCl method for plasmid minipreps. Trends Genet 1:158
Wynne JC, Coffelt TA (1982) Genetics ofArachis hypogaea. In: Patee HE, Young CT (eds) Peanut science and technology. Am Peanut Res Educ Soc, Yoakum, Texas, pp 50–94
Wynne JC, Halward TM (1989) Cytogenetics and genetics ofArachis. Crit Rev Plant Sci 8:189–220
Young CT, Waller GR, Hammons RO (1973) Variations in total amino-acid content of peanut meal. J Am Oil Chem Soc 50:521–523
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Communicated by G. E. Hart
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Halward, T., Stalker, H.T. & Kochert, G. Development of an RFLP linkage map in diploid peanut species. Theoret. Appl. Genetics 87, 379–384 (1993). https://doi.org/10.1007/BF01184927
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DOI: https://doi.org/10.1007/BF01184927