Summary
The effect of cold or isolation stress on neuroinvasiveness and virulence was investigated in mice inoculated with an attenuated WNV (WN-25) strain.
The WN-25 variant differed from the parent strain by its inability to kill mice after I.P. injection though it was able to immunize even after injection with low doses of virus. Exposure of inoculated mice for 5 minutes a day to cold water (1±0.5 °C) for 8 days resulted in 60% mortality, while in non-stressed infected mice no death was observed. Cold or isolation stress increased the virus level in the brain to 8.9 and 7.4 log 10 PFU as compared to no virus in the infected control. Moreover, it was found that virus level in the spleen of stressed mice reached 3.4 and 3.7 log 10 PFU respectively, while in non-stressed mice no virus was detected. The virus which was isolated from the brain of moribund stressed mice was extremely virulent: I.P. inoculation of as little as 10 PFU caused death to normal non-stressed mice.
We suggest that cold or isolation stress conditions in mice inoculated with an attenuated strain induce a selection process. The virus which was isolated from the brain of stressed mice changes its virulence and kills like wild type WNV.
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References
Akov Y, Halevy M, Lustig S (1987) Neuroinvasiveness of West Nile virus strains and the use of the blood brain barrier (BBB). VII. Int Congr Virology, Edmonton, Alberta, Canada, p 74
Albrect P (1968) Pathogenesis of neurotropic arbovirus infections. Curr Top Microbiol Immunol 43: 44–91
Ben-Nathan D, Heller ED, Perek M (1977) The effect of starvation on antibody production of chicks. Poul Sci 56: 1468–1471
Ben-Nathan D, Feuerstein G (1988) Effect of cold or isolation stress on mortality of mice to West Nile virus. Annual Meeting of the Israel Society for Microbiology. Isr J Med Sci, p 393
Ben-Nathan D, Feuerstein G (1988) Stress enhancement of viral encephalitis. FASEB 72nd Annual Meeting Las Vegas, NV, USA, p 935
Chamberlain RW (1980) Epidemiology of arthopod-borne togaviruses: the role of arthopods as hosts and vectors and of vertebrate hosts in natural transmission cycles. In: Schlesinger RW (ed) The togaviruses biology. Structure replication. Academic Press, New York, pp 175–227
Dulbeco R, Vogt M (1956) Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med 99: 167–182
Friedman SB, Glasgow LA (1966) Psychologic factor and resistance to infections disease. Pediatr Clin North Am 13: 315–335
Friedman SB, Glasgow LA, Adir R (1970) Differential susceptibility to viral agent in mice housed alone or in group. Phychosom Med 32: 285–299
Goldblum N, Sterk VV, Paderski B (1954) West Nile Fever: the clinical features of the disease and the isolation of West Nile virus from the blood of nine human cases. Am J Hyg 59: 89–103
Halevy M, Lustig S, Akov Y (1988) Neuroinvasiveness and replication in murine macrophages of two West Nile virus (WNV) strains. Annual Meeting Israel Society for Microbiology, 22, p 148
Hayes CG, Bagar S, Ahmed T, Chowdhry MA, Reisen WK (1982) West Nile virus in Pakistan. I. Sero-epidemiological studies in Pungab province. Trans R Soc Trop Med Hyg 76: 431–435
Jensen MM, Rasmussen AF (1963) Stress and susceptibility to viral infections II. Sound stress and susceptibility to vesicular stomatitis virus. J Immunol 90: 21–23
Jensen MM (1973) Possible mechanisms of impaired interferon production in stressed mice. Proc Soc Exp Biol Med 142: 820–823
Johanson RT, Mims CA (1968) Pathogenesis of viral infection of the nervous system. N Engl J Med 278: 84–92
Johnson T, Lavender JF, Multin E, Rasmussen AF (1963) The influence of avoidance-learning stress on resistance to Coxachie B virus in mice. J Immunol 91: 569–575
Kiecolt-Glaser JK, Speicher C, Holliday JE, Glaser R (1984) Stress and the transformation of lymphocytes by Epstein-Barr virus. J Behav Med 7: 1–11
Kobiler D, Lustig S, Gozes Y, Ben-Nathan D, Akov Y (1989) Sodium dodecyl-sulphate induces a breach in the blood brain barrier and enables a West Nile virus variant to penetrate into mouse brain. Brain Res 496: 314–316
Monath TP (1983) Mode of entry of neurotropic arboviruses into the CNS. Lab Invest 48: 399
Monath TP (1986) Pathobiology of the Flaviviruses. In: Schlesinger S, Schlesinger MJ (eds) The Togaviridae and Flaviviridae. Plenum, New York, pp 375–440
Parillo JE, Fauci AS (1979) Mechanisms of glucocorticoid action on immune processes. Am Rev Pharmacol Toxicol 19: 279–301
Rasmussen AF, March JT, Brill NQ (1957) Increased susceptibility to herpes simplex in mice subjected to avoidance-learning stress or restraint. Proc Soc Exp Biol Med 96: 183–189
Reed LG, Muench M (1938) A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493–497
Sapse AT (1984) Stress cortisol, interferon and stress diseases I. Cortisol as the cause of stress diseases. Med Hypotheses 13: 31–44
Selye H (1950) The physiology and pathology of exposure to stress. Acta, Montreal
Weiner LP, Cole GA, Nathanson N (1970) Experimental encephalitis following peripheral inoculation of West Nile virus in mice of different ages. J Hyg 68: 435–446
Westaway EG, Brinton MA, Gaidamovich SY, Harzinek MC, Igarashi A, Kaariainen L, Lvov DK, Forterfield JS, Russell P, Trent DW (1985) Flaviviridae. Intervirology 24: 183–192
Wolinsky JS, Johanson RT (1980) Role of viruses in chronic neurological diseases. In: Frankel-Cornat H, Wagner RR (eds) Comprehensive virology, vol 16. Plenum, New York, p 257
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Ben-Nathan, D., Lustig, S. & Feuerstein, G. The influence of cold or isolation stress on neuroinvasiveness and virulence of an attenuated variant of West Nile virus. Archives of Virology 109, 1–10 (1989). https://doi.org/10.1007/BF01310513
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DOI: https://doi.org/10.1007/BF01310513