Summary
Rust haustoria isolated from infected leaf tissue strongly bind to ConA. This property was exploited to purify them by affinity chromatography on a ConA-Sepharose macrobead column. Haustoria were obtained with more than 90% purity and yields of up to 50%. Binding of haustoria to the column was partially inhibited by a ConA-specific sugar, methyl α-D-mannopyranoside. Compared to ConA,Lens culinaris agglutinin and wheat germ agglutinin were less efficient affinity ligands. Using ConA-Sepharose, rust haustoria from a variety of sources could be isolated with equal efficiency, indicating that they have similar carbohydrate surface properties. The haustoria maintained their typical shape after the isolation procedure, which suggests a rather rigid wall structure. The morphology of haustoria was characteristic both for a given species and the nuclear condition of the rust mycelium. Electron microscopy of isolated haustoria revealed an intact haustorial wall surrounded by a fibrillar layer presumably derived from the extrahaustorial matrix. The matrix thus appears to represent a layer with gel-like properties which is rich in ConA-binding carbohydrates and connected to the haustorial wall but not to the host-derived extrahaustorial membrane.
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Abbreviations
- ConA:
-
Concanavalin A
- LCA:
-
Lens culinaris agglutinin
- WGA:
-
wheat germ agglutinin
- FITC:
-
fluorescein isothiocyanate
- DAPI:
-
4′,6-diamidinophenylindol×2 HCl
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Hahn, M., Mendgen, K. Isolation by ConA binding of haustoria from different rust fungi and comparison of their surface qualities. Protoplasma 170, 95–103 (1992). https://doi.org/10.1007/BF01378785
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DOI: https://doi.org/10.1007/BF01378785