Abstract
Pyruvate halogen analogs, 3-fluoropyruvate and 3-bromopyruvate, are toxic towardClostridium acetobutylicum. After mutagenesis with nitrosoguanidine, mutants resistant to these compounds were selected. In a normal batch culture regulated at pH 4.8, mutants quickly initiated the acid-solvent transition, accumulating more acetoin and lactate than the wild type strain. The maximum rate of specific glucose uptake was higher in the mutants than in the wild type: 1.76 h−1 and 0.9 h−1 respectively. When the pH was uncontrolled, mutants converted glucose into solvents, essentially butanol, while the wild-type strain ceased its fermentation at the acidogenic stage. Enzymatic investigations revealed that acetate kinase, butyrate kinase, and acetoacetate decarboxylase activities decreased sooner in the mutants than in the parent strain.
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El Kanouni, A., Junelles, A.M., Janati-Idrissi, R. et al. Clostridium acetobutylicum mutants isolated for resistance to the pyruvate halogen analogs. Current Microbiology 18, 139–144 (1989). https://doi.org/10.1007/BF01569561
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DOI: https://doi.org/10.1007/BF01569561