Abstract
A search was made for lymphocyte antigens associated with resistance or susceptibility to the T-cell lymphoma induced by the herpes virus of Marek's disease (MD), the experimental model for Burkitt's lymphoma of humans. Antisera were produced by reciprocal immunization with whole blood between an MD-resistant and susceptible line of chickens compatible at the major histocompatibility complex (MHC), and were tested against lymphocytes of both lines. The lymphocytes were not agglutinated, immobilized, or lysed, but their ability to evoke graft-versus-host (GVH) splenomegaly was reduced. This inhibitory activity was line-specific, and these sera had a maximum limiting effect on GVH splenomegaly at a dilution of 1/50 and a minimum at 1/800 dilution. A test based on the differential limitation of GVH splenomegaly by a pair of alloantisera was used to identify the antigens in F1 and F2 generations. The segregation results established a locus,Ly-4, with two codominant alleles,Ly- 4a andLy-4 b.Ly-4 is distinct from theA, B, orC blood group loci and from theBu-1 locus determining B-cell antigens, but may be linked to theTh-1 locus determining T-cell antigens (recombination frequency of 32 percent). Tentative evidence was obtained from comparisons of homozygous F2 and F3 progeny for association of theLy-4 allele characteristic of the susceptible line with increased incidence of MD.
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References
Briles, W.E., Stone, H.A., and Cole, R.K.: Marek's disease: Effects ofB histocompatibility alloalleles in resistant and susceptible chicken lines.Science 195:193–195, 1977
Bubbers, J.E., Blank, K.J., Freedman, H.A., and Lilly, F.: Mechanisms of the H-2 effect on viral leukemogenesis.Scand. J. Immunol. 6:533–539, 1977
Crone, M., Koch, C., and Simonsen, M.: The elusive T cell receptor.Transplant Rev. 10:36–56, 1972
Crone, M. and Simonsen, M.: Allo-aggression in chickens. II. Cellular expression of the AA gene products.Acta Path. Microbiol. Scand. Sect. C. 84:319–324, 1976
Donnelly, N., Brand, A., and Gilmour, D.G.: Surface alloantigens of bursal and thymic lymphocytes in the chicken.Fed. Proc. 35:768, 1974
Donnelly, N., Brand, A., and Gilmour, D.G.: Bursal and thymic alloantigen expression in lymphoid tissues of the chicken.In W.H. Hildemann and A.A. Benedict (eds.):Immunologic Phytogeny, Plenum Press, New York, 1975
Fredericksen, T.:Chicken Lymphocyte Antigens. Doctoral thesis, University of Alberta, 1976
Fredericksen, T., Longenecker, B.M., Pazderka, F., Stone, H.A., and Ruth, R.F.: A fourth lymphocyte antigen system in the chicken demonstrated by inhibition of GVH splenomegaly.Fed. Proc. 34:1033, 1975
Gilmour, D.G., Brand, A., Donnelly, N., and Stone, H.A.:Bu-1 andTh-1, two loci determining surface antigens of B or T lymphocytes in the chicken.Immunogenetics 3:549–563, 1976
Hansen, M.P., Van Zandt, J.N., and Law, G.R.J.: Differences in susceptibility to Marek's disease in chickens carrying two differentB locus blood group alleles.Poult. Sci. 46:1268, 1967
Hudson, L. and Payne, L.N.: An analysis of the T and B cells of Marek's disease lymphomas of the chicken.Nature (New Biol.) 241:52–53, 1973
Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. Springer-Verlag, New York, 1975
Longenecker, B.M., Pazderka, F., Stone, H.A., Gavora, J.S., and Ruth, R.F.:In ovo assay for Marek's disease virus and turkey herpesvirus.Infect. Immun. 11:922–931, 1975
Longenecker, B.M., Pazderka, F., Gavora, J.S., Spencer, J.L., and Ruth, R.F.: Lymphoma induced by herpes virus: resistance associated with a major histocompatibility gene.Immunogenetics 3:401–407, 1976
Lydyard, P.M. and Ivanyi, J.: The role of opsonization in antithymocyte globulin-induced suppression of graft-versus-host reactions in chick embryos.Transplantation 17:400–404, 1974
Martin, W.J.: Assay for the immunosuppressive capacity of antilymphocyte serum. I. Evidence for opsonization.J. Immunol. 103:979–989, 1969
Martin, W.J. and Miller, J.F.A.P.: Site of action of antilymphocyte globulin.Lancet 2:1285–1287, 1967
Palladino, M.A., Gilmour, D.G., Scafuri, A.R., Stone, H.A., and Thorbecke, G.J.: Immune response differences between two inbred lines of chickens identical at the major histocompatibility complex.Immunogenetics 5:253–259, 1977
Pazderka, F., Longenecker, B.M., Law, G.R.J., and Ruth, R.F.: The major histocompatibility complex of the chicken.Immunogenetics 2:101–130, 1975a
Pazderka, F., Longenecker, B.M., Law, G.R.J., Stone, H.A., and Ruth, R.F.: Histocompatibility of chicken populations selected for resistance to Marek's disease.Immunogenetics 2:93–100, 1975b
Richie, E.R., Monie, H.J., Trentin, J.J., and Taub, R.N.: Inhibition of the graft-versus-host reaction. III. Alteredin vivo distribution of spleen cells pretreated with Fab fragments of anti-thymocyte globulin.Transplantation 19:115–120, 1975
Rouse, B.T., Wells, R.J.H., and Warner, N.L.: Proportion of T and B lymphocytes in lesions of Marek's disease: theoretical implications for pathogenesis.J. Immunol. 110:534–539, 1973
Schierman, L.W. and Nordskog, A.W.: Relationship of erythrocyte to leukocyte antigen in chickens.Science 137:620–622, 1962
Schierman, L.W. and Nordskog, A.W.: Evidence for a second blood group histocompatibility system in chickens.Transplantation 3:44–48, 1965
Stone, H.A.:Use of Highly Inbred Chickens in Research. U.S. Dept. Agric, A.R.S. Tech. Bull. No. 1514, 1975
Taub, R.N. and Lance, E.M.: Effects of heterologous antilymphocyte serum on the distribution of51Cr-labelled lymph node cells in mice.Immunology 15:633–642, 1968
Wong, S.Y., Pazderka, F., Longenecker, B.M., Law, G.R.J., and Ruth, R.F.: Immobilization of lymphocytes at surfaces by alloantibodies.Immunol. Commun. 1:597–613, 1972
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Fredericksen, T.L., Longenecker, B.M., Pazderka, F. et al. A T-cell antigen system of chickens: Ly-4 and Marek's disease. Immunogenetics 5, 535–552 (1977). https://doi.org/10.1007/BF01570512
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DOI: https://doi.org/10.1007/BF01570512