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Statistical analysis of alamethicin channels in black lipid membranes

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Summary

Pore formation of alamethicin has been studied by the analysis of steadystate fluctuations of single-pore conductances. An aggregation model is proposed where transitions to the next higher or lower pore state occur by uptake or release of one monomer. It is assumed that alamethicin forms an elongated loop in the bilayer. The main voltage-dependent step is the insertion of this monomer into the membrane after complexation with a cation. This mechanism is equivalent to dipole orientation in an electric field. Pore formation is restricted by the energy required to enlarge the channel in the membrane.

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References

  • Bamberg, E., Läuger, P. 1973. Channel formation kinetics of gramicidin A in lipid bilayer membranes.J. Membrane Biol. 11:177

    Google Scholar 

  • Bamberg, E., Läuger, P. 1974. Temperature-dependent properties of gramicidin A channels.Biochim. Biophys. (Biomembranes) (In press)

  • Bean, R. C., Shepherd, W. C., Chan, H., Eichner, J. T. 1969. Discrete conductance fluctuations in lipid bilayer protein membranes.J. Gen. Physiol. 53:741

    PubMed  Google Scholar 

  • Benz, R., Stark, G., Janko, K., Läuger, P. 1973. Valinomycin-mediated ion transport through neutral lipid membranes: Influence of hydrocarbon chain length and temperature.J. Membrane Biol. 14:339

    Google Scholar 

  • Cherry, R. J., Chapman, D., Graham, D. E. 1972. Studies of the conductance changes induced in biomolecular lipid membranes by alamethicin.J. Membrane Biol. 7:325

    Google Scholar 

  • Ehrenstein, G., Lecar, H., Nossal, R. 1970. The nature of the negative resistance in bimolecular lipid membranes containing excitability inducing material.J. Gen. Physiol. 55:119

    PubMed  Google Scholar 

  • Eisenberg, M. 1972. Voltage gateable ionic pores induced by alamethicin in black lipid membranes. Ph. D. Thesis. California Institute of Technology, Pasadena, Calif.

    Google Scholar 

  • Eisenberg, M., Hall, J. E., Mead, C. A. 1973. The nature of the voltage-dependent conductance induced by alamethicin in black lipid membranes.J. Membrane Biol. 14:143

    Google Scholar 

  • Finkelstein, A., Holz, R. 1973. Aqueous pores created in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B.In: Membranes, A Series of Advances. G. Eisenman, editor. Vol. II, p. 377. Marcel Dekker, Inc., New York

    Google Scholar 

  • Gingell, D. 1973. Membrane permeability change by aggregation of mobile glycoprotein units.J. Theoret. Biol. 38:677

    Google Scholar 

  • Gordon, L. G. M., Haydon, D. A. 1972. The unit conductance channel of alamethicin.Biochim. Biophys. Acta 255:1014

    PubMed  Google Scholar 

  • Hladky, S. B., Haydon, D. A. 1970. Discreteness of conductance changes in biomolecular lipid membranes in the presence of certain antibiotics.Nature 225:451

    PubMed  Google Scholar 

  • Melling, J., McMullen, A. I. 1974. Abstracts of the IAMS Meeting, Tokyo, September 1974. p. 2421

  • Mueller, P., Rudin, D. O. 1968. Action potentials induced in bimolecular lipid membranes.Nature 217:713

    PubMed  Google Scholar 

  • Payne, J. W., Jakes, R., Hartley, B. S. 1970. The primary structure of alamethicin.Biochem. J. 117:757

    PubMed  Google Scholar 

  • Urry, D. W. 1971. The gramicidin A transmembrane channel: A proposedπ (L, D) helix.Proc. Nat. Acad. Sci. 68:672

    PubMed  Google Scholar 

  • Urry, D. W. 1972. Protein conformation in biomembranes: Optical rotation and absorption of membrane suspensions.Biochim. Bophys. Acta 265:115

    Google Scholar 

  • Urry, D. W., Goodall, M. C., Glickson, J. D., Mayers, D. F. 1971. The gramicidin A transmembrane channel: Characteristics of head-to-head dimerizedπ (L, D) helices.Proc. Nat. Acad. Sci. 68:1907

    PubMed  Google Scholar 

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Boheim, G. Statistical analysis of alamethicin channels in black lipid membranes. J. Membrain Biol. 19, 277–303 (1974). https://doi.org/10.1007/BF01869983

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  • DOI: https://doi.org/10.1007/BF01869983

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