Abstract
Callus tissue of different grapevines (Vitis spp.) was inoculated withPlasmopara viticola. Short, highly-branched hyphae with necrosis, and long hyphae with heavy sporulation were observed on resistant and susceptible callus respectively. Thin-layer chromatography and spectrophotometric analysis showed that resistant callus contained greater quantities of gallocatechin derivatives than susceptible callus. Regression analysis between the field disease rating of each variety and its gallocatechin derivatives content indicated 92.2% correlation. Histochemical studies showed that, after infection withP. viticola, flavonoids appeared in the superficial cell walls of the callus, to a lesser degree on susceptible callus than on resistant callus. At a late stage of infection, the superficial cells of resistant callus were suberized, which did not occur in susceptible callus. This study showed that the preformed gallocatechin derivatives, the induced flavonoids and suberized superficial cells might play a role in the resistance of grapevine callus tissue to this fungus.
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Abbreviations
- CallusI :
-
Callus ofV. riparia var. Gloire de Montpellier
- CallusV :
-
Callus ofV. vinifera var. Grenache
- TLC:
-
Thin Layer Chromatography
- var.:
-
variety
- GAD:
-
Gallic acid derivatives
- GD:
-
gallocatechin derivatives
- RC:
-
resistant callus
- SC:
-
susceptible callus
References
Aist JR (1983) Structure responses as resistance mechanisms. In: Bailey JA, Deverall BJ (eds) The dynamics of host defense (pp 33–70). Academic Press, Australia
Andary C (1975) Contribution à l'étude botanique, chimique et pharmacodynamique d'Orobanche rapum-genistœ Thuill (Orobanchacées). Ph.D. Thesis, Université de Montpellier I, France
Beckman CH and Talboy PW (1981) Anatomy of resistance. In: Wace ME, Bell AA, Beckman CH (eds) Fungal wilt disease of plants (pp 487–521). Academic Press, New York
Boubals D (1959) Contribution à l'étude des causes de la résistance des vitacées au mildiou de la vigne (Plasmopara viticola (B. et C.) Berl. et T.) et de leur mode de transmission héréditaire. Ann Amel Plantes 1:5–233
Brammall RA and Higgins VJ (1988) A histological comparison of fungal colonization in tomato seedlings susceptible or resistant toFusarium crown and root rot disease. Can J Bot 66:915–925
Cho EK and Goodman RM (1979) Strains of soybean mosaic virus: classifcation based on virulence in resistant soybean cultivars. Phytopathology 69:467–470
Dai GH, Andary C, Mondolot-Cosson L and Boubals D (1995a) Histochemical responses of leaves ofin vitro plantlets ofVitis spp. to infection withPlasmopara viticola. Phytopathology 85:149–154
Dai, GH, Andary C, Mondolot-Cosson L and Boubals D (1994) Polyphenols and resistance of grapevines (Vitis spp) to downy mildew (Plasmopara viticola). Acta Horticulturae 381:763–766
Dai GH, Andary C, Mondolot-Cosson L and Boubals D (1995b) Histochemical studies on the interaction between the grapevines (Vitis spp.) and downy mildew (Plasmopara viticola). Physiol Mol Plant Pathol 46:177–188
Friend J (1981) Plant phenolics, lignification and plant disease. In: Reinhold L, Harborne JB, Swain T (eds) Progress in Phytochemistry, Vol 7 (pp 197–261). Pergamon Press, Oxford
Galzy R (1962) Confirmation de la nature virale du court-noué de la vigne et essais de thermothérapie sur des culturesin vitro. CR Acad Sci 253:706–708
Hariri B, Sallé G and Andary C (1991) Involvement of flavonoids in the resistance of two poplar cultivars to mistletoe (Viscum album L.). Protoplasma 162:20–26
Ingram DS (1977) Application in plant pathology. In: Street HE (ed.) Plant Tissue and Cell Culture (pp 463–500). University of California Press, Berkeley
Jensen WA (1962) Botanical Histochemistry. Freeman, San Francisco, California, pp 264
McMurrouch I and McDowell J (1978) Chromathographic separation and automated analysis of flavanols. Analyst Biochem 91: 92–100
Morel G (1944) Le développement du mildiou sur des tissus de vigne cultivésin vitro. CR Acad Sci 218:50–52
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15:473–497
Neu R (1956) A new reagent for differentiating and determining flavones on paper chromatograms. Naturwissenschaften 43:82
Ride JP (1983) Cell walls and other structural barriers in defense. In: Callow JA, Wiley J (eds) Biochemical plant pathology (pp 215–236). Ltd, New York
Sarkar SK and Howarth RE (1976) Specificity of the vanillin test for flavanols. Agric Food Chem 24:317–320
Sequeira L, Gaard G and Dezoeten GA (1977) Interaction of bacteria and host cell walls: its relation to mechanisms of induced resistance. Physiol Plant Pathol 10:43–50
Tripathi SC and Dixit SN (1977) Fungitoxic properties ofRosa chinensis Jacq. Experientia 33(2):207–209
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Dai, G.H., Andary, C., Mondolot-Cosson, L. et al. Involvement of phenolic compounds in the resistance of grapevine callus to downy mildew (Plasmopara viticola). Eur J Plant Pathol 101, 541–547 (1995). https://doi.org/10.1007/BF01874479
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DOI: https://doi.org/10.1007/BF01874479