Abstract
A linkage and association analysis was made on 14 Italian families with recurrent migraine. We analyzed five chromosomal regions surrounding the candidate genes 5HT1D (1p36.3–34.3), 5HT1B (6q13), 5HT2A (13q14–21), 5HT transporter (17q11.2–12), CACNLB1 (17q11.2–22) and FHM (19p13), using 29 DNA polymorphic markers. All two-point lod scores were negative, and the x2 sib-pair analyses were not significant, thus indicating the probable exclusion of these regions as sites of migraine genes in our population.
Sommario
È stata condotta un'analisi di linkage ed associazione su 14 famiglie italiane con ricorrenza di emicrania. Abbiamo analizzato cinque regioni cromosomiche attorno ai geni candidati 5HT1D (1p36.3-34.3), 5HT1B (6q13), 5HT2A (13q14-21), 5HT transporter (17q11.2-12), CACNLB1 (17q11.2-22) e FHM (19p13), usando 29 marcatori polimorfici del DNA.
Tutti i valori dei lod-score a due punti erano negatitivi, i x2 della sib-pair analysis non erano significativi, escludendo così la presenza di geni per l'emicrania nella popolazione in esame nelle regioni analizzate.
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References
Buchwalder A, Welch SK, Peroutka SJ.Exclusion of 5HT 2A and 5HT 2C receptor genes as candidate genes for migraine. Headache, 1996; 36: 254–258.
Gardner K, Ptacek LJ, Hoffman EP.A new locus for hemiplegic migraine is on Chromosome 1q31. 8th Congress of the International Headache Society, 1997 (oral presentation).
Gyapay G, Morisette J, Vignal A, et al.The 1993–94 Genethon human genetic linkage map. Nat Genet, 1994; 7: 246–339.
Headache Classification Committee of the Headache Society.Classification and diagnostic criteria for headache disorders, cranial neuralgias and facial pain. Cephalalgia, 1988; 8(suppl. 7): 1–96.
Hovatta I, Kallela M, Farkkila M, Peltonen L.Familial migraine: exclusion of the susceptibility gene from the reported locus of Familial Hemiplegic Migraine on 19p. Genomics, 1994; 23: 707–709.
Iles DE, Segers B, Sengers RCA, et al.Genetic mapping of the b1- and g- subunits of the human skeletal muscle L-type voltage-dependent calcium channel on chromosome 17q and exclusion as candidate genes for malignant hyperthermia susceptibility. Hum Mol Genet, 1993, 2: 863–868.
Joutel A, Bousser MG, Biousse V, et al.A gene for Familial Hemiplegic Migraine maps to chromosome 19. Nat Genet, 1993; 5: 40–45.
Joutel A, Ducros A, Vahedi K, et al.Genetic heterogeneity of Familial Hemiplegic Migraine. Am J Hum Genet, 1994; 15: 200–204.
Lance JW.Migraine: clinical aspects. In: Lance J.W. ed. “Mechanism and Management of Headache”, Butterworth-Heinemann Ltd University Press, Cambridge, 1993.
May A, Ophoff RA, Terwindt GM, et al.Familial Hemiplegic Migraine locus on 19p13 is involved in the common forms of migraine with and without aura. Hum Genet, 1995; 96: 604–608.
Miller SA, Dykess SS, Polesky HF.A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res, 1988; 6: 1215.
Mochi M, Sangiorgi S, Cortelli P, et al.Testing models for genetic determination in migraine. Cephalalgia, 1993; 13: 389–394.
Mochi M, Valentino ML, Montagna P.A null allele for the afm175xg3 marker at locus d17s795 caused by a primer binding failure. Int J Legal Med, 1996; 109: 104–106.
Montagna P, Valentino ML, Cortelli P, et al.Genetic mapping in familial migraine. It J Neurol Sci, 1995; 7(suppl): 52.
Nyholt DR, Curtain RP, Gaffney PT, et al.Migraine association and linkage analysis of the human 5-hydroxytryptamine (5HT 2A)receptor gene. Cephalalgia, 1996; 16: 463–467.
Ophoff RA, Van Eijk R, Sandkuijl LA, et al.Genetic heterogeneity of Familial Hemiplegic Migraine. Genomics, 1994; 22: 21–26.
Ophoff RA, Terwindt GM, Vergouwe MN, et al.Familial Hemiplegic Migraine and episodic ataxia type 2 are caused by mutations in the Ca 2+ channel gene CACNL1A4. Cell, 1996; 87: 543–552.
Peroutka SJ.Serotonin receptor subtypes. Their evolution and clinical relevance. CNS Drugs, 1995; 4(suppl.1): 18–28.
Powers PA, Liu S, Hogan K, Gregg RG.Skeletal muscle and brain isoforms of a beta-subunit of human voltage-dependent calcium channels are encoded by a single gene. J Biol Chem, 1992; 267: 22967–22972.
Sandkuijl LA.Analysis of affected sib-pairs using information from extended families. In: Elston R.C., Spence M.M., Hodge S.E., et al eds. Multipoint Mapping and Linkage based upon affected pedigree members: genetic analysis workshop 6. New York, Alan R. Liss, 1989.
Weber JL, May PE.Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet, 1989; 44: 388–396.
Williamson R, Bowcock A, Kidd K, et al.Report of the DNA committee and catalogues of cloned and mapped genes, markers formatted for PCR and DNA polymorphisms. HGM11. Cytogenet Cell Genet, 1991; 58/3–4: 1190–1198.
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This work was supported by MURST (40%) and CNR grant 2785 e 96.03122.CT04
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Monari, L., Mochi, M., Valentino, M.L. et al. Searching for migraine genes: exclusion of 290 cM out of the whole human genome. Ital J Neuro Sci 18, 277–282 (1997). https://doi.org/10.1007/BF02083304
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DOI: https://doi.org/10.1007/BF02083304