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Accuracy of pregnancy/non pregnancy diagnosis in zebu and crossbred cattle and Murrah buffaloes by milk progesterone determination post insemination

Fiabilite Du Diagnostic De Gestion Ou Non Chez Des Zebus, Des Metis Et Des Bufflesses Murrah Par Determination De La Progesterone Du Lait Apres Insemination

Precision En El Diagnostico De Prenez/No Prenez En Vacas Cebu, Sus Cruces Y Vacas Bufalo De La Raza Murrah

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Summary

Milk samples collected on days 0, 6, 20, 22 and 24 post insemination from Sahiwal and Tharparkar (zebu) cows, Karan Swiss (Sahiwal × Brown Swiss) and Karan Fries (Tharparkar × Holstein Friesian) crossbred cows and Murrah buffaloes were analysed for progesterone by enzyme-linked immunosorbent assay to determine the accuracy of pregnancy and non pregnancy diagnosis in each of the 3 groups of animals. The accuracy of pregnancy diagnosis was markedly improved in cows when the milk progesterone values on days 20, 22 and 24 were considered together, and a further improvement when the levels on days 0, 6, 20, 22 and 24 were considered together particularly in crossbred cows. In contrast, buffaloes exhibited the highest degree of accuracy from samples collected on day 24, and improvement in accuracy was minimal when values were considered for all 5 days, viz. 0, 6, 20, 22 and 24 post insemination. Overall, the accuracy of positive pregnancy diagnosis was greatest in zebu cows (90·9%) followed by crossbred cows (86·6%) and Murrah buffaloes (76·5%) when milk progesterone values on all 5 days were considered. The accuracy for negative pregnancy diagnosis was 100% on all days. The results suggest that there is a greater variability in oestrous cycle length in cattle than in buffaloes, and embryonic mortality is greater in buffaloes than in cattle.

Résumé

La progestérone a été recherchée dans des échantillons de lait prélevés avant et 6, 20, 22 et 24 jours après insémination de vaches (zébu) Tharparkar et Sahiwal, de métis Suisse Karan (Sahiwal × Brown Suisse), Karan Fries (Tharparkar × Holstein Frison) et de bufflesses Murrah, en utilisant ELISA pour déterminer la fiabilité du diagnostic de gestation ou non dans chacun des 3 groupes d'animaux. La fiabilité du diagnostic de gestation a été nettement améliorée chez les vaches lorsque les valeurs de progestérone du lait aux 20e 22e et 24e jours ont été regroupées, et encore améliorée lorsque les valeurs avant insémination et aux 6e, 203, 22e et 24e jours ont été examinées ensemble, particulièrement chez les métis. En revanche les échantillons provenant des bufflesses ont montré le degré le plus élevé de fiabilité lorsqu'ils ont été prélevés le 24e jour et l'amélioration a été minimale quand les valeurs ont été considérées sur les 5 jours, c'est-à-dire avant et aux 6e, 20e, 22e et 24e jours après insémination. En général, la fiabilité du diagnostic positif de gestation était plus importante chez les vaches zébus (90,9 p.100), suivies par les métis (86,6 p.100) et les bufflesses Murrah (76,5 p.100) lorsque les valeurs de progestérone du lait étaient examinées sur les 5 jours. La fiabilité pour le diagnostic de non gestation était de 100 p.100 sur tous les jours. Les résultats font penser que la longueur du cycle oestral varie plus chez les bovins que chez les bufflesses et que la mortalité des embryons est plus élevée chez les buffles que chez les bovins.

Resumen

Por medio de la prueba ELISA, se examinaron muestras para detectar progesterona en leche colectada los dias 0, 6, 20, 22 y 24 después de la inseminación, de 4 grupos de animales: cebú Sahiwal, cebú Tharparkar, Karan Suizo (Sahiwal × Pardo Suizo), Karan Fries (Tharparkar × Holstein Friesian) y vacas búfalo de la raza Murrah. La precisión en el diagnóstico de preñez se mejoró marcadamente cuando los valores de progesterona en la leche tomados en los días 20, 22 y 24 se consideraron juntos, mejorándose aún más cuando los niveles de progesterona tomados los días 0, 6, 20, 22 y 24 se consideraron juntos, particularmente en vacas cruzadas. En contraste, las vacas búfalo presentaron la precisión más alta cuando las muestras tomadas el día 24 fueron examinadas, siendo la precisión encontrada mínima cuando los valores fueron considerados a los cinco días pos-inseminación. La precisión en el diagnóstico fue más alta en vacas cebú (90·9%), seguida de las vacas cruzadas (86·6%) y por los búfalos (76·5%), cuando se consideraron los valores tomardo los 5 días. La precisión para el diagnóstico de preñez negativa fue 100% todos los días. Los resultados sugieren que existe una differencia significativa entre la duración del estro en ganado vs búfalos, presentándose una mayor mortalidad embrionaria en búfalos que en ganado bovino.

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Kaul, V., Prakash, B.S. Accuracy of pregnancy/non pregnancy diagnosis in zebu and crossbred cattle and Murrah buffaloes by milk progesterone determination post insemination. Trop Anim Health Prod 26, 187–192 (1994). https://doi.org/10.1007/BF02241083

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