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Conservation of a 31-bp bovine subrepeat in centromeric satellite DNA monomers ofCervus elaphus and other cervid species

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Abstract

A centromeric satellite DNA clone was isolated from the genome of the European red deer (Cervus elaphus hippelaphus) and designated Ce-Pst1. This clone was localized to the centromeric region of all red deer chromosomes with the exception of a single pair of metacentric autosomes and the Y chromosome. DNA sequence analysis of the 806-bp Ce-Pst1 clone showed 73.0–78.9% sequence homology to four previously isolated cervid centromeric satellite DNA clones, suggesting that the Ce-Pst1 clone is yet another member of the major cervid centromeric satellite DNA family. Using a DNA sequence comparison system, internal 31-bp tandem subrepeats were found in the Ce-Pst1 clone as well as in the other previously reported cervid centromeric satellite DNA monomer sequences. A 31-bp consensus sequence was constructed for each cervid monomer clone and shown to be highly homologous to the 31-bp subrepeat consensus sequence found in bovine 1.715 centromeric satellite DNA. The identification of internal subrepeats in the satellite monomers studied could suggest that amplification of an ancestral 31-bp DNA sequence may have contributed to the genesis of major cervid centromeric satellite DNA. The homology between the 31-bp subrepeats found in cervid and bovid centromeric satellite DNAs substantiates the theory that amplification of this 31-bp DNA sequence may have occurred before the evolutionary separation of these two families 20–25 million years ago.

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References

  • Blackburn EH (1991) Structures and function of telomeres.Nature 350: 569–573.

    Article  PubMed  Google Scholar 

  • Bogenberger JM, Neumaier PS, Fittler F (1985) The Muntjak satellite 1A sequence is composed of 31-base-pair internal repeats that are highly homologous to the 31-base-pair subrepeats of the bovine satellite 1.715.Eur J Biochem 148: 55–59.

    Article  PubMed  Google Scholar 

  • Bogenberger JM, Neitzel H, Fittler F (1987) A highly repetitive DNA component common to all Cervidae: its organization and chromosomal distribution during evolution.Chromosoma 95: 154–161.

    Article  PubMed  Google Scholar 

  • Catasti P, Gupta G, Garcia AE et al. (1994) Unusual structures of the tandem repetitive DNA sequences located at human centromeres.Biochemistry 33: 3819–3831.

    Article  PubMed  Google Scholar 

  • Ferrer N, Azorin F, Villasante A, Gutierrez C, Abad JP (1995). Centromeric dodeca-satellite DNA sequences form foldback structures.J Mol Biol 245: 8–21.

    PubMed  Google Scholar 

  • Gaillard C, Doly J, Cortadas J, Bernardi G (1981) The primary structure of bovine satellite 1.715.Nucleic Acids Res 9: 6069–6082.

    PubMed  Google Scholar 

  • Grady DL, Ratliff RL, Robinson DL et al. (1992) Highly conserved repetitive DNA sequences are present at human centromeres.Proc Natl Acad Sci USA 89: 1695–1699.

    PubMed  Google Scholar 

  • Gustavsson I, Sundt CO (1968) Karyotypes in five species of deer (Alces alces L.,Capreolus capreolus L.Cervus elaphus L.Cervus nippon temm. andDama dama L.).Hereditas 60: 233–247.

    PubMed  Google Scholar 

  • Horz W, Altenburger W (1981) Nucleotide sequence of mouse satellite DNA.Nucleic Acids Res 9: 683–696.

    PubMed  Google Scholar 

  • Hsu TC, Pathak S, Chen TR (1975) The possibility of latent centromeres and a proposed nomenclature system for total and whole arm translocations.Cytogenet Cell Genet 15: 41–49.

    PubMed  Google Scholar 

  • Huff V, Jaffe N, Saunders GF et al. (1995). WT1 exon I deletion/insertion mutations in Wilms' tumor patients, associated with di- and trinucleotide repeats and deletion hotspot consensus sequences.Am J Hum Genet 56: 84–90.

    PubMed  Google Scholar 

  • Irwin DM, Wilson AC (1990) Concerted evolution of ruminant stomach lysozymes.J Biol Chem 265: 4944–4952.

    PubMed  Google Scholar 

  • Jeffreys AJ, Wilson V, Thein S (1985) Hypervariable minisatellite regions in human DNA.Nature 314: 67–73.

    Article  PubMed  Google Scholar 

  • Jobse C, Buntjer JB, Haagsma N et al. (1995) Evolution and recombination of bovine DNA repeats.J Mol Evol 41: 277–283.

    Article  PubMed  Google Scholar 

  • Krowczynska AM, Rudders RA, Krontiris TG (1990) The human minisatellite consensus at brakpoints of oncogene translocations.Nucleic Acids Res 18: 1121–1127.

    PubMed  Google Scholar 

  • Lee C, Ritchie DBC, Lin CC (1994) A tandemly repetitive, centromeric DNA sequence from the Canadian woodland caribou (Rangifer tarandus caribou): its conservation and evolution in several deer species.Chrom Res 2: 293–306.

    Article  PubMed  Google Scholar 

  • Lee C, Li X, Jabs EW, Court D, Lin CC (1995) Human gamma X satellite DNA: an X chromosome specific centromeric DNA sequence.Chromosoma 104: 103–112.

    PubMed  Google Scholar 

  • Lima-de-Faria A, Arnason U, Widegren B et al. (1984) Conservation of repetitive DNA sequences in deer species studied by southern blot transfer.J Mol Evol 20: 17–24.

    Article  PubMed  Google Scholar 

  • Lima-de-Faria A, Arnason U, Widegren B et al. (1986) DNA cloning and hybridization in deer species supporting the chromosome field theory.Biosystems 19: 185–212.

    Article  PubMed  Google Scholar 

  • Lin CC, Sasi R, Fan Y-S, Chen Z-Q (1991) New evidence for tandem chromosome fusions in the karyotypic evolution of Asian muntjacs.Chromosoma 101: 19–24.

    Article  PubMed  Google Scholar 

  • Lin CC, Sasi R, Lee C, Fan YS, Court D (1993) Isolation and identification of a novel tandemly repeated DNA sequence in the centromeric region of human chromosome 8.Chromosoma 102: 333–339.

    Article  PubMed  Google Scholar 

  • Modi WS (1993) Rapid, localized amplification of a unique satellite DNA family in the rodentMicrotus chrotorrhinus.Chromosoma 102: 484–490.

    Article  PubMed  Google Scholar 

  • Nowak R (1994) Mining treasures from ‘junk DNA’.Science 263: 608–610.

    PubMed  Google Scholar 

  • Pech M, Streeck RE, Zachau HG (1979) Patchwork structure of a bovine satellite DNA.Cell 18: 883–893.

    Article  PubMed  Google Scholar 

  • Plucienniczak A, Skowronski J, Jaworski J (1982) Nucleotide sequence of bovine 1.715 satellite DNA and its relation to other bovine satellite sequences.J Mol Biol 158: 293–304.

    Article  PubMed  Google Scholar 

  • Scherthan H (1990) The localization of the repetitive telomeric sequence (TTAGGG)n in two Muntjac species and implications for their karyotypic evolution.Cytogenet Cell Genet 53: 115–117.

    PubMed  Google Scholar 

  • Scherthan H (1991) Characterization of a tandem repetitive sequence cloned from the deerCapreolus capreolus and its chromosomal localisation in two muntjac species.Hereditas 115: 43–49.

    PubMed  Google Scholar 

  • Scherthan H (1995) Chromosome evolution in muntjac revealed by centromere, telomere and whole chromosome paint probes. In: Brandham PE, Bennett MD eds.Kew Chromosome Conference IV. Kew: Royal Botanic Gardens, pp. 267–280.

    Google Scholar 

  • Scott KM, Janis CM (1987) Phylogenetic relationships of the Cervidae, and the case for a superfamily ‘Cervoide’. In: Wemmer CM ed.Biology and Management of the Cervidae. Washington, DC: Smithsonian Institute Press, pp. 3–20.

    Google Scholar 

  • Simpson CD (1984) Artiodactyls. In: Anderson, S., Jones JK, eds.Orders and Families of Recent Mammals of the World. New York: John Wiley & Sons, pp. 563–587.

    Google Scholar 

  • Singer MF (1982) Highly repeated sequences in mammalian genomes.Int. Rev Cytol 76: 67–112.

    PubMed  Google Scholar 

  • Steinmetz M, Stephan D, Lindahl FK (1986) Gene organization and recombinational hotspots in the murine major histocompatibility complex.Cell 44: 895–904.

    Article  PubMed  Google Scholar 

  • Sundquist WI (1991) The structures of telomeric DNA. In: Eckstein F, Lilley DMJ, eds.Nucleic Acids and Molecular Biology, Vol. 5. Berlin: Springer, pp. 1–24.

    Google Scholar 

  • Taparowsky EJ, Gerbi SA (1982) Sequence analysis of bovine satellite I DNA (1.715 gm/cm3).Nucleic Acids Res 10: 1271–1281.

    PubMed  Google Scholar 

  • Tinoco Jr I, Borer PN, Dengler B et al. (1973)Nature New Biology 264: 40–41.

    Google Scholar 

  • Yu LC, Lowensteiner D, Wong EFK, Sawada I, Mazrimas J, Schmid C (1986) Localization and characterization of recombinant DNA clones derived from the highly repetitive DNA sequences in the Indian muntjac cells: Their presence in the Chinese muntjac.Chromosoma 93: 521–528.

    Article  PubMed  Google Scholar 

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Correspondence to C. C. Lin.

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accepted for publication by H. C. Macgregor

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Lee, C., Lin, C.C. Conservation of a 31-bp bovine subrepeat in centromeric satellite DNA monomers ofCervus elaphus and other cervid species. Chromosome Res 4, 427–435 (1996). https://doi.org/10.1007/BF02265049

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  • DOI: https://doi.org/10.1007/BF02265049

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