Abstract
The sulfate-reducing bacteriumDesulfobulbus propionicus oxidized sulfide, elemental sulfur, and sulfite to sulfate with oxygen as electron acceptor. Thiosulfate was reduced and disproportionated exclusively under anoxic conditions. When small pulses of oxygen were added to washed cells in sulfide-containing assays, up to 3 sulfide molecules per O2 disappeared transiently. After complete oxygen consumption, part of the sulfide reappeared. The intermediate formed was identified as elemental sulfur by chemical analysis and turbidity measurements. When excess sulfide was present, sulfur dissolved as polysulfide. This process was faster in the presence of cells than in their absence. The formation of sulfide after complete oxygen consumption was due to a disproportionation of elemental sulfur (or polysulfide) to sulfide and sulfate. The uncoupler tetrachlorosalicylanilide (TCS) and the electron transport inhibitor myxothiazol inhibited sulfide oxidation to sulfate and caused accumulation of sulfur. In the presence of the electron transport inhibitor 2-n-heptyl-4-hydroxyquinoline-N-oxide (HQNO), sulfite and thiosulfate were formed. During sulfur oxidation at low oxygen concentrations, intermediary formation of sulfide was observed, indicating disproportionation of sulfur also under these conditions. It is concluded that sulfide oxidation inD. propionicus proceeds via oxidation to elemental sulfur, followed by sulfur disproportionation to sulfide and sulfate.
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Abbreviations
- HQNO :
-
2-n-Heptyl-4-hydroxyquinoline-N-oxide
- NEM :
-
N-Ethylmaleimide
- TCS :
-
Tetrachlorosalicylanilide
References
Bacon M, Ingledew WJ (1989) The reductive reactions ofThiobacillus ferrooxidans on sulphur and selenium. FEMS Microbiol Lett 58:189–194
Bak F, Cypionka H (1987) A novel type of energy conservation involving fermentation of inorganic sulphur compounds. Nature 326:891–892
Beffa T, Berzcy M, Aragno M (1991) Chemolithotrophic growth on elemental sulfur (So) and respiratory oxidation of So byThiobacillus versutus and another sulfur-oxidizing bacterium. FEMS Microbiol Lett 84:285–290
Beffa T, Berzcy M, Aragno M (1992) Inhibition of respiratory oxidation of elemental sulfur (So) and thiosulfate inThiobacillus versutus and another sulfur-oxidizing bacterium. FEMS Microbiol Lett 90:123–127
Beffa T, Fischer C, Aragno M (1993) Inhibition of respiratory oxidation of sulfur compounds by intact cells ofThiobacillus denitrificans (strain RT) grown on thiosulfate. Experentia 49:910–914
Canfield DE, Thamdrup B (1994) The production of34S-depleted sulfide during bacterial disproportionation of elemental sulfur. Science 266:1973–1975
Chan CW, Suzuki I (1993) Quantitative extraction and determination of elemental sulfur an stoichiometric oxidation of sulfide to elemental sulfur byThiobacillus thiooxidans. Can J Microbiol 39:1166–1168
Chen K Y, Gupta K (1973) Formation of polysulfides in aqueous solution. Environ Lett 4:187–200
Cypionka H (1994) Sulfate transport. Methods Enzymol 243:3–14
Cypionka H, Pfennig N (1986) Growth yields ofDesulfotomaculum orientis with hydrogen in chemostat culture. Arch Microbiol 143:366–369
Dannenberg S, Kroder M, Dilling W, Cypionka H (1992) Oxidation of H2, organic compounds and inorganic sulfur compounds coupled to reduction of O2 or nitrate by sulfate-reducing bacteria. Arch Microbiol 158:93–99
Dilling W, Cypionka H (1990) Aerobic respiration in sulfate-reducing bacteria. FEMS Microbiol Lett 71:123–128
Fossing H, Jørgensen BB (1990) Isotope exchange reactions with radiolabeled sulfur compounds in anoxic seawater. Biogeochemistry 9:223–245
Hazeu W, Batenburg-Van der Vegte WH, Bos P, Van der Pas RK, Kuenen JG (1988) The production and utilization of intermediary elemental sulfur during the oxidation of reduced sulfur compounds byThiobacillus ferrooxidans. Arch Microbiol 150:574–579
Kelly DP (1989) Physiology and biochemistry of unicellular sulfur bacteria. In: Schlegel HG, Bowien B (eds) Biology of autotrophic bacteria. Science Tech, Madison, pp 193–217
Kletzin A (1994) Sulfur oxidation and reduction in Archaea: sulfur oxygenase/-reductase and hydrogenases from the extremely thermophilic and facultative anaerobic archaeonDesulfurolobus ambivalens. System Appl Microbiol 16:534–543
Krämer M, Cypionka H (1989) Sulfate formation via ATP sulfurylase in thiosulfate- and sulfite-disproportionating bacteria. Arch Microbiol 151:232–237
Lovley DR, Phillips EJP (1994) Novel processes for anaerobic sulfate production from elemental sulfur by sulfate-reducing bacteria. Appl Environ Microbiol 60:2394–2399
Pronk JT, Meulenberg R, Hazeu W, Bos P, Kuenen JG (1990) Oxidation of reduced inorganic sulfur compounds by acidophilic thiobacilli. FEMS Microbiol Rev 75:293–306
Schauder R, Müller E (1993) Polysulfide as a possible substrate for sulfur-reducing bacteria. Arch Microbiol 160:377–382
Suzuki I, Chan CW, Vilar R, Takeuchi TL (1993) Sulfur and sulfide oxidation byThiobacillus thiooxidans. In: Thorma AE, Wey JE, Lakshmanan VL (eds) Biohydrometallurgical technologies. Minerals, Metals and Materials Society, pp 109–116
Thamdrup B, Finster K, Würgler Hansen J, Bak F (1993) Bacterial disproportionation of elemental sulfur coupled to chemical reduction of iron or manganese. Appl Environ Microbiol 59:101–108
Thierbach G, Reichenbach H (1981) Myxothiazol, a new inhibitor of the cytochromeb-c 1 segment of the respiratory chain. Biochim Biophys Acta 638:282–289
Widdel F, Pfennig N (1982) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. 2. Incomplete oxidation of propionate byDesulfobulbus propionicus gen. nov., sp. nov. Arch Microbiol 131:360–365
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Dedicated to Prof. Dr. Dr. h.c. Norbert Pfennig on the occasion of his 70th birthday
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Fuseler, K., Cypionka, H. Elemental sulfur as an intermediate of sulfide oxidation with oxygen byDesulfobulbus propionicus . Arch. Microbiol. 164, 104–109 (1995). https://doi.org/10.1007/BF02525315
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DOI: https://doi.org/10.1007/BF02525315