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Culturing satellite cells from living single muscle fiber explants

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Summary

Conventional methods for isolating myogenic (satellite) cells are inadequate when only small quantities of muscle, the tissue in which satellite cells reside, are available. We have developed a tissue culture system that reliably permits isolation of intact, living, single muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and differentiation of satellite cells from each fiber; and removal of the fiber from culture for analysis.

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Authors and Affiliations

  1. Muscle Cell Biology Group, MRC Clinical Research Building, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, England

    J. David Rosenblatt & Terence A. Partridge

  2. Department of Physiology, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, Ontario, Canada

    Alison I. Lunt & David J. Parry

Authors
  1. J. David Rosenblatt
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  2. Alison I. Lunt
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  3. David J. Parry
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  4. Terence A. Partridge
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Rosenblatt, J.D., Lunt, A.I., Parry, D.J. et al. Culturing satellite cells from living single muscle fiber explants. In Vitro Cell Dev Biol - Animal 31, 773–779 (1995). https://doi.org/10.1007/BF02634119

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  • DOI: https://doi.org/10.1007/BF02634119

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