Abstract
Purpose: This study was undertaken to correlate the follicular levels of interleukin (IL)-1α, IL-2, tumor necrosis factor-α (TNF-α), and leukotriene (LT) B4 with oocyte maturity, fertilization, and achievement of pregnancy.
Methods: The material was obtained from 22 women undergoing IVF, 8 of whom became pregnant and 14 of whom did not.
Results: All of the studied cytokines and LT B4 were found in follicular fluids, but there were no significant differences according to oocyte maturity, fertilization, embryo quality, and achievement of pregnancy. On the other hand, a significant positive correlation was found between IL-1α and TNF-α, IL-1α, and LT B4 as well as between TNF-α and LT B4 in follicular fluids with subsequently fertilized oocytes.
Conclusions: It seems that IL-1α, TNF-α and LT B4 may take part in the process of follicle wall degradation, and their follicular correlations may suggest more optimal follicular and oocyte development and maturation.
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Oppenheim JJ, Ruscetti FW, Faltynek C: Cytokines.In Basic and Clinical Immunology, DP Stites, AI Terr (eds). East Norwalk, CT, Appleton and Lange, 1991, 7th ed, p 78
Simon C, Polan ML: Cytokines and reproduction. West J Med 1994;160:425–429
Zolti M, Ben-Rafael Z, Meirom R, Shemesh M, Bider D, Mashiach S, Apte RN: Cytokine involvement in oocytes and early embryos. Fertil Steril 1991;56:256–272
Tabibzadeh S: Cytokines and the hypothalamic-pituitary-ovarian-endometrial axis. Hum Reprod 1994;9:947–967
Feldman E, Haberman S, Abisogun AO, Reich R, Levran D, Maschiach S, Zuckermann H, Rudak E, Dor J, Tsafriri A: Arachidonic acid metabolism in human granulosa cells: Evidence for cyclooxygenase and lipoxygenase activity in vitro. Hum Reprod 1986;1:353–356
Watanabe H, Nagai K, Yamaguchi M, Ikenoue T, Mori N: Concentration of interleukin-1β correlates with prostaglandin E2 and F2α in human preovulatory follicular fluid. Hum Reprod 1994;9:9–12
Wang LJ, Brännström M, Robertson SA, Norman RJ: Tumor necrosis factor α in the human ovary: Presence in follicular fluid and effects on cell proliferation and prostaglandin production. Fertil Steril 1992;58:934–940
Lipner H: Mechanisms of mammalian ovulation. In The Physiology of Reproduction, E Knobil, J Neil (eds). New York, Raven Press, 1988, p 447
Tarlatzis B, Pados G, Bontis J, Lagos S, Grimbizis G, Spanos E, Mantalenakis S: Ovarian stimulation with buserelin/HMG/HCG: Prospective randomized study of short versus long protocol. Hum Reprod 1993;8:807–812
Tarlatzis BC, DeCherney AH: Semen preparation for IVF.In Foundations of In Vitro Fertilization, CM Fredericks, J Paulson, AH DeCherney (eds). Washington, DC, Hemisphere, 1987, p 60
Pados G, Tarlatzis BC, Bontis J, Lagos S, Papadimas J, Spanos S, Mantalenakis S: Evaluation of different ovarian stimulation protocols for in vitro fertilization. Gynecol Endocrinol 1995;9:103–112
Wang LJ, Norman RJ: Concentrations of immunoreactive interleukin-1 and interleukin-2 in human preovulatory follicular fluid. Hum Reprod 1992;7:147–150
Godfrey RW, Johnson WJ, Hoffstein ST: Recombinant tumor necrosis factor and interleukin-1 both stimulate human synovial cell arachidonic acid release and phospholipid metabolism. Biochem Biophys Res Commun 1987;142:235–241
Kokia E, Hurwitz A, Ricciarelli E, Tedeschi C, Resnick CE, Mitchell MD, Adashi EY: Interleukin-1 stimulates ovarian prostaglandin bio-synthesis: Evidence for heterologous contact independent cell-cell interaction. Endocrinology 1992;130:3095–3097
Wang LJ, Robertson S, Seamark RF, Norman RJ: Lymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro. J Clin Endocrinol Metab 1991;72:824–831
Zolti M, Bider D, Seidman DS, Maschiach S, Ben-Rafael Z: Cytokine levels in follicular fluid of polycystic ovaries in patients treated with dexamethasone. Fertil Steril 1992;57:501–504
Priddy AR, Killick SR, Elstein M, Morris J, Sullivan M, Patel L, Elder MG: Ovarian follicular fluid eicosanoid concentrations during the pre-ovulatory period in humans. Prostaglandins 1989;38:197–202
Knapp HR: Reduced allergen-induced nasal congestion and leukotriene synthesis with an orally active 5-lipoxygenase inhibitor. N Engl J Med 1990;323:1745–1748
Rabe T, Przylipiak A, Grunwald K, Parta S, Przylipiak M, Runnebaum B: Action of leukotriene B4 in human granulosa cells in vitro. Mol Hum Reprod 1995;1:1881–1884
Loukides JA, Loy RA, Edwards R, Honig J, Visintin I, Polan ML: Human follicular fluids contain tissue macrophages. J Clin Endocrinol Metab 1990;71:1363–1367
Barak V, Mordel N, Horzer H, Zajicek G, Treves AJ, Laufer N: The correlation of interleukin-1 and tumour necrosis factor to oestradiol, progesterone and testosterone levels in periovulatory follicular fluid of in vitro fertilization patients. Hum Reprod 1992;7:462–464
Huyser C, Fourie FLR, Bosmans E, Levay PF: Interleukin-1β, interleukin-6, and growth hormone levels in human follicular fluid. J Assist Reprod Genet 1994;11:193–202
DeCherney AH, Tarlatzis BC, Laufer N: Follicular development: Lessons learned from human in vitro fertilization. Am J Obstet Gynecol 1985;153:911–923
Atkins RC: Interleukin-1 in crescentic glomerulonephritis. Kidney Int 1995;48:576–586
Oppenheim JJ, Ruscetti FW, Faltynek C: Cytokines.In Basic and Clinical Immunology, DP Stites, AI Terr (eds). East Norwalk, CT, Appleton and Lange, 1991, 7th ed, p 78
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Bili, H., Tarlatzis, B.C., Daniilidis, M. et al. Cytokines in the human ovary: Presence in follicular fluid and correlation with leukotriene B4 . J Assist Reprod Genet 15, 93–98 (1998). https://doi.org/10.1007/BF02766833
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DOI: https://doi.org/10.1007/BF02766833