Abstract
Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertility-restoring gene(Rf i) of upland cotton. A total of 425 arbitrary 10-mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC3 segregating population of (0-613-2R X Simian No. 3). Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of (Zhong 12A-1 × 0-613-2R)F2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV-15300 was linked with the fertility-restoring gene (Rf1) with a recombination value of 13.0±2.57%.
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References
Jia, Z. C., Development of 104-7A CMS lines in cotton,Chinese Cotton; (in Chinese), 1990, 17(6): 1–4.
Wang, X. D., Zhang, T. Z., Pan, J. J., Genetic, basis of fertility restoration to cytoplasmic male sterile lines available in upland cotton. I. Restorer genes and their effects,Scientia Agricultura Sinica (in Chinese), 1996, 29(5): 32.
Justus, N., Leinweber, C. L., A heritable partially male sterile character in cotton,J. Hered., 1960(51): 191.
Michelmore, R. W., Paran, I. K., Bulked segregant analysis: A rapid nethod to detect markers in specific genomic regions by using segregating population,Proc. Natl. Acad. Sci. USA, 1991(88): 9828.
Guo, W. Z., Zhang, T. Z., Pan, J. J., Analysis of RAPD fingerprin ing on main cotton cultivars in China,Journal of Agricultural Biotechnology (in Chinese), 1996, 4(2): 129.
Song, T. M., Li, J. X.,Plant Cytogenetics, Beijing: Science Press, 1994, 112–120.
Reinisch, A. J., Dong, J. M., Brubaker, C. L.et al., A detailed RFLP map of cotton,Gossypium hirsutum ×Gossypium barbadense: Chromosome organization and evolution in a disomic polyploid genome,Genetics, 1994(138): 829.
Ji, J., Wang, G., Beihassen, E.et al., Studies on CMS restores geneRf 1 probe inHelianthus annus L. using bulked DNA-RAPD method,Science in China Ser. C (in Chinese), 1996, 26(4): 377.
Shi, Y. G., Zheng, L., Li, J. S.et al., Mapping CMS-S restores gereRf 3 with RFLPs and RAPDs,Acta Agronomica Sinica (in Chinese), 1997, 23(1): 1.
Delourme, R., Bouchereau, A., Hubert, N.et al., Identification of RAPD markers linked to a fertility restorer gene for theOgura radish cytoplasmic male sterility of rapeseed (Brassica napus L.).Theor. Appl. Genet., 1994(88): 741.
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Guo, W., Zhang, T., Pan, J. et al. Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton. Chin.Sci.Bull. 43, 52–54 (1998). https://doi.org/10.1007/BF02885512
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DOI: https://doi.org/10.1007/BF02885512