Abstract
Platelets play important role in precipitating ischaemic myocardial syndromes in many ways. One of the consequences of ischaemic diseases is excessive generation of oxygen derived free radicals that have numerous pathophysiological consequences. Platelet pro-oxidant enzyme, xanthine oxidase is one of the sources of generation of free radicals. In the present paper, we report the effect of administration of vitamin E along with aspirin on the levels of platelet xanthine oxidase and extent of free radical mediated damage in the patients reperfused after myocardial infarction.
Our findings show that administration of 400 mg. vitamin E for six days along with 80 mg. aspirin has an excellent anti-oxidant effect as evidenced by reduced platelet xanthine oxidase activity and lowering of malondialdehdye (MDA) levels which is an index of the extent of free radical mediated damage.
Similar content being viewed by others
References
Bolli R, Patel BS, Jeroudi M.O, Lai EK, Mc.Cay PB (1988) Demonstration of free radical generation in “stunned” myocardium of intact dogs with the use of spin trap alpha phenyl N-tert.-butyl nitrone. J. Clin. Invest.; 82: 476–485.
Zweier JL. (1988) Measurement of super oxide derived free radicals in the reperfused heart: Evidence for a free radical mechanism of reperfusion injury. J. Biol. Chem.; 263: 1353–1357.
Loun E, Factor SM, Van Hoeven KH, Weu WH, Zhao M, Dawood F, Lin P. (1994) Effect of oxygen free radicals and scavengers on the cardiac extra cellular collagen matrix during ischaemia-reperfusion. Canad. J. Cardiol.; 10: 203–213.
Alexander RW. (1995) Hypertension and pathogenesis of atherosclerosis. Oxidative stress and the mediation of arterial inflammatory response: a new perspective. Hypertension.; 25: 155–161.
Pandey NR, Kaur G, Chandra M, Sanwal GG, Misra MK. (2000) Enzymatic oxidant and anti-oxidants of human blood platelets in unstable angina and myocardial infarction. Int. J. Cardiol.; 76:33–38.
Yokoyama Y, Parks DA. (1988) Circulating xanthine oxidase: release of xanthine oxidase from isolated rat liver. Gasteroenterology.; 94: 607–611.
Muenzer J, Weisbach EC, Wolfe SM. (1975) Oxygen consumption of human blood platelets. I. Effect of thrombin. Biochim. Biophys. Acta.; 376:237–242.
Roussos GG. (1967). Xanthine oxidase from bovine small intestine. In Grossman L. and Moldave K. Editors. Methods in Enzymology. XII A: 5–16. Academic Press;
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem.; 193:265–275.
Ohkawa H, Ohishi N, Yagi K. (1979) Assay of lipid peroxidation in animal tissues by thiobarbituric acid raction. Anal. Biochem.; 95:351–358.
Esterbauer H, Cheeseman KH. (1990) Determination of aldehydic lipid peroxidation products: Malondialdehyde and 4-hydoxynonenal. Methods in Enzymol.; 186: 407–421.
Draper HH, Handley M. (1990) Malondialdehyde determination, an index of lipid per-oxidation. Methods in Enzymology.; 186: 421–431.
Armstrong D, Browne R. (1994) The analysis of free radicals, lipid peroxidases, antioxidant enzymes and compounds related to oxidative stress as applied to the clinical chemistry laboratory. Free Rad. Diagnos. Med.; 366: 46.
Bressler NM, Brockman MJ, Marcus AJ. (1979) Concurrent studies of oxygen consumption and aggregation in stimulated human platelets. Blood.; 53: 167–178.
Fitzgerald DJ, Roy L, Catella F, Fitzgerald GA. (1986) Platelet activation in unstable coronary disease. New. Eng. J. Med.; 315:983–989.
ISIS-2 (Second International Study of Infarct Survival) Collaborative group. Randomised trial of intravenous streptokinase, oral aspirin, both, or neither among 17187 cases of suspected acute myocardial infarction 1988: ISIS-2. Lancet; 2: 349–360.
Burton GW, Ingold KU. (1981) Auto oxidation of biological molecules. 1. The anti oxidant activity of vitamin E and related chain breaking phenolic anti oxidants in vitro. J. Am. Chem. Soc.; 103: 6472–6477.
Sandhya P, Thomas GA, Donald DH. Vitamin E supplementation in the prevention of coronary heart disease. Mayo. Clin. Proc. 2001; 76: 1–7.
Kleijnen J, Knipschild P, Riet GT. (1989) Vitamin E and Cardiovascular disease. Eur. J. Clin. Pharmacol.; 37: 541–544.
American Family Physician. Low dose aspirin vs. vitamin E in cardiovascular disease. 2001.
Steiner M, Glantz M, Lekos A. (1995) Vitamin E plus aspirin compared with aspirin alone in patients with transient attacks. Am. J. Clin. Nutr.; 62: 6–7.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Raghuvanshi, R., Chandra, M., Misra, P.C. et al. Effect of vitamin E on the platelet xanthine oxidase and lipid peroxidation in the patients of myocardial infarction. Indian J Clin Biochem 20, 26–29 (2005). https://doi.org/10.1007/BF02893037
Issue Date:
DOI: https://doi.org/10.1007/BF02893037