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Human autoantibodies: probes for nucleolus structure and function

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Virchows Archiv B

Conclusions

Four distinct nucleolar proteins or RNA-protein complexes have recently been identified as targets of human autoimmune antibodies. These autoantigens areRNA polymer-ase I, PM-Scl (a particle possibly related to preribosomes), 7-2 RNP andfibrillarin (a U3-RNP associated protein). The four different nucleolar autoantigens could be assigned to distinct nucleolar subcompartments by light and electron microscopic immunocytochemistry. RNA polymerase I was located in the fibrillar centers, PM-Scl antigen and 7-2 RNP in the granular component and fibrillarin in the dense fibrillar component. Experimental evidence suggests that these naturally occurring antibodies could be helpful tools in further studying nucleolus structure and functions as well as molecular mechanisms involved in ribosome biogenesis.

From a clinical viewpoint, we believe that it is important to identify the nature of reactive autoantigens in systemic autoimmune diseases in order to answer questions concerning the mechanisms which render conserved ubiquitous cellular proteins immunogenic. Revealing such mechanisms in return could give clues with regard to the etiology of certain systemic rheumatic diseases. Ribosome biogenesis, a highly dynamic process with its many well-defined intermediate biological steps related to specific nuclear structures could be amenable for such studies.

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Authors and Affiliations

  1. Department of Dermatology, University of Erlangen-Nürnberg, D-8520, Erlangen, Federal Republic of Germany

    Georg Reimer

  2. Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague, CSSR

    Ivan Raška

  3. W.M. Keck Foundation Autoimmune Disease Center, Scripps Clinic and Research Foundation, 92037, La Jolla, California, USA

    Eng M. Tan

  4. Institute of Zoology I, University of Würzburg, D-8700, Würzburg, Federal Republic of Germany

    Ulrich Scheer

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  1. Georg Reimer
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  2. Ivan Raška
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  4. Ulrich Scheer
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Reimer, G., Raška, I., Tan, E.M. et al. Human autoantibodies: probes for nucleolus structure and function. Virchows Archiv B Cell Pathol 54, 131–143 (1987). https://doi.org/10.1007/BF02899205

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