Abstract
Total and polysome-bound ribosomes and the uptake and incorporation of3H-uridine and14C-leucine were examined in dividing microspores and in pollen grains isolated from anthers of 6 different developmental stages. Direct evidence was obtained that the formation of cytoplasm of the vegetative cell following microspore division is related to a rapid activation of RNA and protein synthesis and of ribosomes in differentiating pollen. Total ribosomes associated with gametophytic programme rose about 10times and the process of differentiation was accompanied by a rapid increase in uptake capacity of pollen grains for both uridine and leucine. Pollen development after cytoplasm synthesis and starch deposition continued by pollen maturation, which was characterized by a decline in RNA synthesis, dissociation of polysomes and by a further rise of transport activity of pollen grain wall for exogenous substrates, indicating probable pollen adaptation for utilization of metabolites from the degenerating tapetal cytoplasm.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhojwani, S. S., Dunwkll, J. M., Sunderland, N.: Nucleic-acid and protein contents of embryogénic tobacco pollen. - J. exp. Bot.24: 863–871, 1973.
Čarková-Balatková, V., Hrabětová, E., Tupý, J.: Effect of some mineral ions on pollen tube growth and release of proteins in culture. - Biol. Plant.22: 294–302, 1980.
Čapková, V., Hrabětová, E., Tupý, J., Říhová, L.: Amino acid uptake and protein synthesis in cultured tobacco pollen. - Biochem. Physiol. Pflanzen178 : in press.
Martin, T. E.: A simple general method to determine the proportion of active ribosomes in eucaryotic cells. - Exp. Cell Res.80: 496–498, 1973.
Mascarenhas, J. P.: The biochemistry of angiosperm pollen development. - Bot. Rev.41: 259–314, 1975.
Mascarenhas, J. P., Bell, E.: RNA synthesis during development of the male gametophyte ofTradescantia. -Dev. Biol.21: 476–490, 1970.
Stanley, R. G., Linskens, H. F.: Pollen. Biology, Biochemistry, Management. - Springer Yerlag, Berlin-Heidelberg-New York 1974.
Steffensen, D. M.: Synthesis of ribosomal RNA during growth and division inLilium. - Exp. Cell Res.44: 1–12, 1966.
Sunderland, N., Dunwell, J. M.: Anther and pollen culture. - In:Street, H. E. (ed.): Plant Tissue and Cell Culture. Pp. 223–586. Blackwell Scientific Publications, Oxford 1978.
Süss, J., Tupý, J.: Kinetics of uridine uptake and incorporation into RNA in tobacco pollen culture.- Biol. Plant.24: 72–79, 1982.
Tupý, J.: RNA synthesis and polysome formation in pollen tubes. - Biol. Plant.19: 300–308, 1977.
Tupý, J.: Alterations in polyadenylated RNA during pollen maturation and germination. - Biol. Plant.24: 331–340, 1982.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tupý, J., Süss, J., Hrabětová, E. et al. Developmental changes in gene expression during pollen differentiation and maturation inNicotiana tabacum L . Biol Plant 25, 231–237 (1983). https://doi.org/10.1007/BF02902110
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02902110