Abstract
The controversy regarding the identity of a major cellulosomal component type from two different strains ofClostridium thermocellum has been resolved. The principal cellobiohydrolase, subunit S8, from the cellulosome of strain YS has been demonstrated to be synonymous with cellulase component Ss (CelS) from the cellulosome of ATCC strain 27405. This component is not related to any other cellulosomal subunit or cloned endoglucanase in this organism.
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Lamed, R., Setter, E., Kenig, R., and Bayer, E. A. (1983),Biotechnol. Bioeng. Symp. 13, 163–181.
Lamed, R. and Bayer, E. A. (1988),Advan. Appl. Microbiol. 33, 1–46.
Kohring, S., Weigel, J., and Mayer, F. (1990),Appl. Environ. Microbiol. 56, 3798–3804.
Wu, J. H. D. and Demain, A. L. (1988), inBiochemistry and Genetics of Cellulose Degradation, Aubert, J.-P., P. Beguin and J. Millet, Eds. pp. 117–131. Academic Press, London.
Lamed, E., Naimark, J., Morgenstern, E., and Bayer, E. A. (1987),J. Bacteriol.,169, 3792–3800.
Lamed, R., Morag (Morgenstern), E., Mor-Yosef, O., and Bayer, E. A. (1991),Curr. Microbiol. 22, 27–33.
Bond, K. and Stutzenberger, F. (1990),Lett. Appl. Microbiol. 67, 605–610.
Gilbert, H. J. and Hazlewood, G. P. (1993),J. Gen. Microbiol. 139, 187–194.
Hazlewood, G. P. and Gilbert, H. J., inThe Clostridia and Biotechnology, Woods, D. R., eds, Butterworths, Stoneham, MA, in press.
Morag, E., Halevy, I., Bayer, E. A., and Lamed, R. (1991),J. Bacteriol. 173, 4155–4162.
Fauth, U., Romaniec, M. P. M., Kobayashi, T., and Demain, A. L. (1991),Biochem. J. 279, 67–73.
Wang, W. K., Kruus, K., and Wu, J. H. D. (1993),J. Bacteriol. 175, 1293–1302.
Worag (Morgenstern), E., Bayer, E. A., and Lamed, R. (1992),Enzyme Microb. Technol. 14, 289–292.
Matsudaira, P. (1987),J. Biol. Chem. 262, 10,035–10,038.
Cleveland, D. W., Fischer, S. G., Kirschner, M. W., and Laemmli, U. K. (1977),J. Biol. Chem. 252, 1102–1106.
Bayer, E. A., Setter, E., and Lamed, R. (1985),J. Bacteriol. 163, 552–559.
Hazlewood, G. P., Romaniec, M. P. M., Davidson, K., Grépinet, O., Béguin, P., Millet, J., Raynaud, O., and Aubert, J.-P. (1988),FEMS Microbiol. Lett. 51, 231–236.
Tokatlidis K., Salamitou, S., Béguin, P., Dhurjati, P., and Aubert, J.-P., (1991),FEBS Lett. 291, 185–188.
Fujino, T., Béguin, P., and Aubert, J.-P. (1992),FEMS Microbiol. Lett. 94, 165–170.
Poole, D. M., Morag, E., Lamed, R., Bayer, E. A., Hazlewood, G. P., and Gilbert, H. J. (1992),FEMS Microbiol. Lett. 99, 181–186.
Bayer, E. A., Kenig, R., and Lamed, R. (1983),J. Bacteriol. 156, 818–827.
Lamed, R., Setter, E., and Bayer, E. A. (1983),J. Bacteriol. 156, 828–836.
Wu, J. H. D., Orme-Johnson, W. H., and Demain, A. L. (1988),Biochemistry 27, 1703–1709.
Salamitou, S., Tokatlidis, K., Béguin, P., and Aubert, J.-P. (1992),FEBS Lett. 304, 89–92.
Gerngross, U. T., Romaniec, M. P. M., Kobayashi, T., Huskisson, N. S., and Demain, A. L. (1993),Mol. Microbiol. 8, 325–334.
Kobayashi, T., Romaniec, M. P. M., Fauth, U., Barker, P. J., and Demain, A. L. (1992),Enzyme Microb. Technol. 14, 447–453.
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Morag, E., Bayer, E.A., Hazlewood, G.P. et al. Cellulase Ss (CelS) is synonymous with the major cellobiohydrolase (subunit S8) from the cellulosome ofClostridium thermocellum . Appl Biochem Biotechnol 43, 147–151 (1993). https://doi.org/10.1007/BF02916439
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DOI: https://doi.org/10.1007/BF02916439