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Ascorbate interacts with sodium selenite to increase glutathione peroxidase activity in selenium-deficient chick duodena cultured in vitro

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Abstract

Duodena from Selenium (Se)/vitamin E-depleted 19 d chick embryos were cultured in vitro for 0–30 h. The addition of sodium selenite to the culture medium was associated with increased selenium-dependent glutathione peroxidase (SeGSHpx) activity after 24 h of incubation. In the absence of Se or in the presence of sodium ascorbate supplementation alone, SeGSHpx activity showed a gradual decline over the same time period. When ascorbate was added, along with sodium selenite, SeGSHpx activity was increased earlier and to a greater extent than in the presence of Se alone. These observations show that ascorbate can influence the metabolism of sodium selenite, resulting in increased SeGSHpx activity.

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Cupp, M.S., Combs, G.F. & Corradino, R.A. Ascorbate interacts with sodium selenite to increase glutathione peroxidase activity in selenium-deficient chick duodena cultured in vitro. Biol Trace Elem Res 20, 87–94 (1989). https://doi.org/10.1007/BF02919101

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  • DOI: https://doi.org/10.1007/BF02919101

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