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Aqueous two-phase systems in biochemical recovery

Systematic analysis, design, and implementation of practical processes for the recovery of protein

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Abstract

The design and application of aqueous two-phase systems for recovery of proteins is reviewed. Reference is made to the properties of polymers and salt forming the systems and their influence on phase separation and partition. The properties of systems important for the design of purification strategies are discussed in relation to the surface properties of proteins. Strategies for the modification of systems for bioaffinity partition are considered including the choice of carrier in terms of molecular type and properties, or by particulate addition. Finally, the scaleup of partition is considered, and the reasons for currently limited adoption at larger scales are elucidated.

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  1. Biochemical Recovery Group, School of Chemical Engineering, University of Birmingham, B15 2TT, Edgbaston, Birmingham, UK

    Jon G. Huddleston & Andrew Lyddiatt

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Huddleston, J.G., Lyddiatt, A. Aqueous two-phase systems in biochemical recovery. Appl Biochem Biotechnol 26, 249–279 (1990). https://doi.org/10.1007/BF02921506

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