Abstract
Cell viability during chronological aging and after apoptotic stimuli in some yeast mutants with altered mitochondrial morphology was followed; a function for the corresponding genes in the apoptotic process was assessed.MDM30 andDNM1, the genes encoding an F-box protein and the dynamin-related GTPase, respectively, are involved in triggering aging and apoptosis. In contrast,YME1, encoding a subunit of the mitochondrial inner membrane i-AAA proteinase complex, has a protective role in these processes.FIS1, the mitochondrial fission gene, might play a protective role after an apoptotic insult while it seems to promote cell death in aging cells.
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Abbreviations
- GFP:
-
green fluorescent protein(s)
- PNPase:
-
polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)
- MT:
-
mitochondrial
References
Arnold I., Wagner-Ecker M., Ansorge W., Langer T.: Evidence for a novel mitochondria-to-nucleus signaling pathway in respiring cells lacking i-AAA protease and the ABC-transporter Md11.Gene 367, 74–88 (2006).
Breckenridge D.G., Stojanovic M., Marcellus R.C., Shore G.C.: Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochromec release to the cytosol.J.Cell Biol. 160, 1115–1127 (2003).
Chan D.C.: Mitochondria: dynamic organelles in disease, aging, and development.Cell 125, 1241–1252 (2006).
Chen H.W., Rainey R.N., Balatoni C.E., Dawson D.W., Troke J.J., Wasiak S., Hong J.S., Mcbride H.M., Koehler C.M., Teitell M.A., French S.W.: Mammalian polynucleotide phosphorylase is an intermembrane space RNase that maintains mitochondrial homeostasis.Mol.Cell.Biol. 26, 8475–8487 (2006).
Durr M., Escobar-Henriques M., Merz S., Geimer S., Langer T., Westermann B.: Nonredundant roles of mitochondria-associated F-box proteins Mfb1 and Mdm30 in maintenance of mitochondrial morphology in yeast.Mol.Biol.Cell 17, 3745–3755 (2006).
Escobar-Henriques M., Westermann B., Langer T.: Regulation of mitochondrial fusion by the F-box protein Mdm30 involves proteasome-independent turnover of Fzol.J.Cell Biol. 173, 645–650 (2006).
Fannjiang Y., Cheng W.C., Lee S.J., Qi B., Pevsner J., Mccaffery J.M., Hill R.B., Basanez G., Hardwick J.M.: Mitochondrial fission proteins regulate programmed cell death in yeast.Genes Dev. 18, 2785–2797 (2004).
Frank S., Gaume B., Bergmann-Leitner E.S., Leitner W.W., Robert E.G., Catez F., Smith C.L., Youle R.J.: The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis.Dev.Cell 1, 515–525 (2001).
Fritz S., Weinbach N., Westermann B.: Mdm30 is an F-box protein required for maintenance of fusion-competent mitochondria in yeast.Mol.Biol.Cell 14, 2303–2313 (2003).
Hales K.G., Fuller M.T.: Developmentally regulated mitochondrial fusion mediated by a conserved, novel, predicted GTPase.Cell 90, 121–129 (1997).
Heath-Engel H.M., Shore G.C.: Mitochondrial membrane dynamics, cristae remodeling and apoptosis.Biochim.Biophys.Acta 1763, 549–560 (2006).
Jagasia R., Grote P., Westermann B., Conradt B.: DRP-1-mediated mitochondrial fragmentation during EGL-1-induced cell death inC. elegans.Nature 433, 754–760 (2005).
James D.I., Parone P.A., Mattenberger Y., Martinou J.C.: hFis1, a novel component of the mammalian mitochondrial fission machinery.J.Biol.Chem. 278, 36373–36379 (2003).
Juhola M.K., Shah Z.H., Grivell L.A., Jacobs H.T.: The mitochondrial inner membrane AAA metalloprotease family in metazoans.FEBS Lett. 481, 91–95 (2000).
Karbowski M., Lee Y.J., Gaume B., Jeong S.Y., Frank S., Nechushtan A., Santel A., Fuller M., Smith C.L., Youle R.J.: Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis.J.Cell Biol. 159, 931–938 (2002).
Labrousse A.M., Shurland D.L., Van Der Bliek A.M.: Contribution of the GTPase domain to the subcellular localization of dynamin in the nematodeCaenorhabditis elegans.Mol.Biol.Cell 9, 3227–3239 (1998).
Lee Y.J., Jeong S.Y., Karbowski M., Smith C.L., Youle R.J.: Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis.Mol.Biol.Cell 15, 5001–5011 (2004).
Leonhard K., Herrmann J.M., Stuart R.A., Mannhaupt G., Neupert W., Langer T.: AAA proteases with catalytic sites on opposite membrane surfaces comprise a proteolytic system for the ATP-dependent degradation of inner membrane proteins in mitochondria.EMBO J. 15, 4218–4229 (1996).
Leonhard K., Stiegler A., Neupert W., Langer T.: Chaperone-like activity of the AAA domain of the yeast Ymel AAA protease.Nature 398, 348–351 (1999).
Leszczyniecka M., Desalle R., Kang D.C., Fisher P.B.: The origin of polynucleotide phosphorylase domains.Mol.Phylogenet.Evol. 31, 123–130 (2004).
Longo V.D., Mitteldorf J., Skulachev V.P.: Programmed and altruistic ageing.Nature Rev.Genet. 6, 866–872 (2005).
Ludovico P., Madeo F., Silva M.: Yeast programmed cell death: an intricate puzzle.IUBMB Life 57, 129–135 (2005).
Manon S., Priault M., Camougrand N.: Mitochondrial AAA-type protease Yme1p is involved in Bax effects on cytochrome-c oxidase.Biochem.Biophys.Res.Commun. 289, 1314–1319 (2001).
Mazzoni C., Herker E., Palermo V., Jungwirth H., Eisenberg T., Madeo F., Falcone C.: Yeast caspase 1 links messenger RNA stability to apoptosis in yeast.EMBO Rep. 6, 1076–1081 (2005).
Mozdy A.D., Mccaffery J.M., Shaw J.M.: Dnm1p GTPase-mediated mitochondrial fission is a multi-step process requiring the novel integral membrane component Fis1p.J.Cell Biol. 151, 367–380 (2000).
Muratani M., Kung C., Shokat K.M., Tansey W.P.: The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing.Cell 120, 887–899 (2005).
Rainey R.N., Glavin J.D., Chen H.W., French S.W., Teitell M.A., Koehler C.M.: A new function in translocation for the mitochondrial i-AAA protease Yme1: import of polynucleotide phosphorylase into the intermembrane space.Mol.Cell Biol. 26, 8488–8497 (2006).
Shaw J.M., Nunnari J.: Mitochondrial dynamics and division in budding yeast.Trends Cell Biol. 12, 178–184 (2002).
Sugioka R., Shimizu S., Tsujimoto Y.: Fzo1, a protein involved in mitochondrial fusion, inhibits apoptosis.J.Biol.Chem. 279, 52726–52734 (2004).
Weber E.R., Hanekamp T., Thorsness P.E.: Biochemical and functional analysis of theYME1 gene product, an ATP and zinc-dependent mitochondrial protease fromS. cerevisiae.Mol.Biol.Cell 7, 307–317 (1996).
Westermann B., Neupert W.: Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis inSaccharomyces cerevisiae.Yeast 16, 1421–1427 (2000).
Yaffe M.P.: The cutting edge of mitochondrial fusion.Nature Cell Biol. 5, 497–499 (2003).
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Palermo, V., Falcone, C. & Mazzoni, C. Apoptosis and aging in mitochondrial morphology mutants ofS. cerevisiae . Folia Microbiol 52, 479–483 (2007). https://doi.org/10.1007/BF02932107
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DOI: https://doi.org/10.1007/BF02932107