Abstract
Like every redox-active compound vitamin E may exert pro-oxidative and anti-oxidative effects depending on the reaction partners present. In this work we evaluated the intensity of oxidative stress produced by a physical exercise through swimming as well as of protecting action of antioxidant vitamins E and C. Antioxidant systems include antioxidant enzymes: superoxide-dismutase (SOD), catalase (CAT), glutathion peroxidase (GSH-Px), as well as of components with an antioxidant action of the reduced glutathion type (GSH) and vitamins E and C. We determine the activities of these enzymes in the erythrocytes and heart homogenate. Our results points out a protective effect against oxidative stress produced by swimming in animals treated with vitamins E and C, which are expressed through the diminution of the malondialdehyde (MDA) quantity both in erythrocytes and in the heart, and through the conservation of GSH content in both products. CAT and GSH-Px activities decrease while that of SOD increases on both tissues, but with different intensities in accordance with the variation of protection degree performed by the vitamin couple on these tissues. The obtained data underline the necessity of intensifying the means of endogenous antiradical defence with exogenous antioxidant vitamins C and E. This study highlights the need of a proper vitamin supplement in organism under stress.
Resumen
Como todos los compuestos redox activos, la vitamina E puede ejercer efectos prooxidativos y antioxidativos, en función de los coparticipantes presentes en la reacción. En este trabajo se evalúa la intensidad del estrés oxidativo producido por el ejercicio físico de la natación, así como la acción protectora de las vitaminas antioxidantes E y C. Los sistemas antioxidantes incluyen las enzimas antioxidantes superóxido-dismutasa (SOD), catalasa (CAT), glutatión-peroxidasa (GSH-Px), así como los compuestos con acción antioxidante tipo glutatión reducido (GSH) y las vitaminas E y C. Se ha determinado la actividad enzimática en eritrocitos y el homogenado cardíaco. Nuestros resultados indican un efecto protector respecto del esfuerzo oxidativo por el ejercicio en animales tratados con vitaminas E y C, que se manifiesta por la disminución de la cantidad de malondialdehido (MDA) sin alteración del contenido de GSH tanto en eritrocitos como en corazón. La actividad CAT y GSH-Px disminuye, mientras que la de SOD aumenta en ambos preparados, aunque con distintas intensidades. Los datos obtenidos remarcan la importancia de intensificar los métodos de defensa endógena antioxidante mediante suplemento de vitaminas exógenas antioxidantes C y E en respuesta a las necesidades del esfuerzo físico.
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Ciocoiu, M., Badescu, M. & Paduraru, I. Protecting antioxidative effects of vitamins E and C in experimental physical stress. J. Physiol. Biochem. 63, 187–194 (2007). https://doi.org/10.1007/BF03165781
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DOI: https://doi.org/10.1007/BF03165781