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  • Food Microbiology
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Production and partial characterization of extracellular peroxidase produced byStreptomyces sp. F6616 isolated in Turkey

Annals of Microbiology volume 59, pages 323–334 (2009)Cite this article

Abstract

Streptomyces sp. F6616 was found to produce higher levels of extracellular peroxidase activity (0.535 U/mL) without any inducers than other actinobacteria which are previously reported. Maximum specific peroxidase activity (6.21 U/mg of protein) was obtained after 72 h of incubation at 30°C in a minimal salt medium (pH 8.0) containing (in wt/v) 0.6% yeast extract and 0.8% ball-milled wheat straw corresponding to a C:N ratio of 4.6:1. Characterization of the peroxidase revealed that the optimal temperature for the enzyme activity, using the standard 2,4-dichlorophenol (2,4-DCP) assay was 50°C, when the enzyme reaction was performed at pH 8.0. A study of the effect of temperature on the stability of peroxidase over time, showed that the enzyme was stable at 50°C, with a half-life of 145 min, while at higher temperature the stability and activity was reduced such that at 60°C the half-life of the enzyme was 30 min. The optimum pH for the activity of the enzyme occurred between pH 9.0 and 10.0. The apparentK m andV max values for the peroxidase preparations were determined to be 1.52 mmol/L and 1.84 U/mg protein, respectively using 2,4-DCP as a substrate. Characterization of the peroxidase activity revealed activity against 2,4-DCP, L-3,4-dihydroxyphenylalanine (L-DOPA), 2,4,5-trichlorophenol and other chlorophenols in the presence of hydrogen peroxide. However, inhibition of peroxidase activity with the addition of potassium cyanide and sodium azide, suggested the presence of heme component in the tertiary structure of the enzyme.

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Authors and Affiliations

  1. Faculty of Arts and Science, Department of Biology, University of Mersin, Çiftlikköy, 33342, Mersin, Turkey

    Münir Tuncer, Ali Kuru & Melahat Isikli

  2. Faculty of Arts and Science, Department of Biology, 19 Mayis University, Kurupelit, 55139, Samsun, Turkey

    Nevzat Sahin & Kamil Isik

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  1. Münir Tuncer
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  2. Ali Kuru
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  3. Nevzat Sahin
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Correspondence to Münir Tuncer.

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Tuncer, M., Kuru, A., Sahin, N. et al. Production and partial characterization of extracellular peroxidase produced byStreptomyces sp. F6616 isolated in Turkey. Ann. Microbiol. 59, 323–334 (2009). https://doi.org/10.1007/BF03178335

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Annals of Microbiology

ISSN: 1869-2044

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